P75794 (PFLE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative pyruvate formate-lyase 3-activating enzyme
EC=1.97.1.4

Alternative name(s):
Formate-C-acetyltransferase-activating enzyme 3
PFL-activating enzyme 3

Gene names

Name:	ybiY
Ordered Locus Names:	b0824, JW0808

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine By similarity.
Catalytic activity	S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the organic radical-activating enzymes family.
Sequence caution	The sequence BAA35512.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW [formate-C-acetyltransferase]-activating enzyme activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	Putative pyruvate formate-lyase 3-activating enzyme		PRO_0000200528
Sites
	Metal binding	25	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	29	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	32	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P75794 [UniParc]. Last modified November 24, 2009. Version 2. Checksum: CA8AFB4C956E732C	FASTA	299	33,038
	10 20 30 40 50 60 MIFNIQRYST HDGPGIRTVV FLKGCSLGCR WCQNPESRAR TQDLLYDARL CLEGCELCAK 70 80 90 100 110 120 AAPEVIERAL NGLLIHREKL TPEHLTALTD CCPTQALTVC GEVKSVEEIM TTVLRDKPFY 130 140 150 160 170 180 DRSGGGLTLS GGEPFMQPEM AMALLQASHE AGIHTAVETC LHVPWKYIAP SLPYIDLFLA 190 200 210 220 230 240 DLKHVADAPF KQWTDGNAAR VLDNLKKLAA AGKKIIIRVP LIQGFNADET SVKAITDFAA 250 260 270 280 290 DELHVGEIHF LPYHTLGINK YHLLNLPYDA PEKPLDAPEL LDFAQQYACQ KGLTATLRG

References

[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC73911.2. AP009048 Genomic DNA. Translation: BAA35512.1. Different initiation.
PIR	H64819.
RefSeq	NP_415345.2. NC_000913.2.
3D structure databases
ProteinModelPortal	P75794.
SMR	P75794. Positions 2-299.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11444N.
IntAct	P75794. 5 interactions.
MINT	MINT-1313356.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000218; EBESCP00000000218; EBESCG00000000177. EBESCT00000017433; EBESCP00000016724; EBESCG00000016489.
GeneID	945445.
GenomeReviews	Gene locus JW0808 in contig AP009048_GR. Gene locus b0824 in contig U00096_GR.
KEGG	ecj:JW0808. eco:b0824.
PATRIC	32116851. VBIEscCol129921_0851.
Organism-specific databases
EchoBASE	EB3243.
EcoGene	EG13470. ybiY.
Phylogenomic databases
eggNOG	COG1180.
GeneTree	EBGT00050000009897.
HOGENOM	HBG554871.
PhylomeDB	P75794.
ProtClustDB	CLSK881195.
Enzyme and pathway databases
BioCyc	EcoCyc:G6427-MONOMER.
Gene expression databases
Genevestigator	P75794.
Family and domain databases
InterPro	IPR012839. Glycyl_radical_activase. IPR001989. Radical_activat_CS. IPR007197. rSAM. [Graphical view]
KO	K04069.
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF000371. PFL_act_enz. 1 hit.
TIGRFAMs	TIGR02494. PFLE_PFLC. 1 hit.
PROSITE	PS01087. RADICAL_ACTIVATING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PFLE_ECOLI

Accession

Primary (citable) accession number: P75794

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 24, 2009
Last modified:	January 25, 2012
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program