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P75794 (PFLE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative pyruvate formate-lyase 3-activating enzyme

EC=1.97.1.4
Alternative name(s):
Formate-C-acetyltransferase-activating enzyme 3
PFL-activating enzyme 3
Gene names
Name:ybiY
Ordered Locus Names:b0824, JW0808
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length299 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine By similarity.

Catalytic activity

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the organic radical-activating enzymes family.

Sequence caution

The sequence BAA35512.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 299299Putative pyruvate formate-lyase 3-activating enzyme
PRO_0000200528

Sites

Metal binding251Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding291Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding321Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
P75794 [UniParc].

Last modified November 24, 2009. Version 2.
Checksum: CA8AFB4C956E732C

FASTA29933,038
        10         20         30         40         50         60 
MIFNIQRYST HDGPGIRTVV FLKGCSLGCR WCQNPESRAR TQDLLYDARL CLEGCELCAK 

        70         80         90        100        110        120 
AAPEVIERAL NGLLIHREKL TPEHLTALTD CCPTQALTVC GEVKSVEEIM TTVLRDKPFY 

       130        140        150        160        170        180 
DRSGGGLTLS GGEPFMQPEM AMALLQASHE AGIHTAVETC LHVPWKYIAP SLPYIDLFLA 

       190        200        210        220        230        240 
DLKHVADAPF KQWTDGNAAR VLDNLKKLAA AGKKIIIRVP LIQGFNADET SVKAITDFAA 

       250        260        270        280        290 
DELHVGEIHF LPYHTLGINK YHLLNLPYDA PEKPLDAPEL LDFAQQYACQ KGLTATLRG 

« Hide

References

[1]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC73911.2.
AP009048 Genomic DNA. Translation: BAA35512.1. Different initiation.
PIRH64819.
RefSeqNP_415345.2. NC_000913.3.
YP_489097.1. NC_007779.1.

3D structure databases

ProteinModelPortalP75794.
SMRP75794. Positions 2-299.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-11444N.
IntActP75794. 5 interactions.
MINTMINT-1313356.
STRING511145.b0824.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73911; AAC73911; b0824.
BAA35512; BAA35512; BAA35512.
GeneID12933841.
945445.
KEGGecj:Y75_p0797.
eco:b0824.
PATRIC32116851. VBIEscCol129921_0851.

Organism-specific databases

EchoBASEEB3243.
EcoGeneEG13470. ybiY.

Phylogenomic databases

eggNOGCOG1180.
HOGENOMHOG000011459.
KOK04069.
OrthoDBEOG63VBWZ.
PhylomeDBP75794.
ProtClustDBCLSK881195.

Enzyme and pathway databases

BioCycEcoCyc:G6427-MONOMER.
ECOL316407:JW0808-MONOMER.

Gene expression databases

GenevestigatorP75794.

Family and domain databases

InterProIPR012839. Glycyl_radical_activase.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF000371. PFL_act_enz. 1 hit.
TIGRFAMsTIGR02494. PFLE_PFLC. 1 hit.
PROSITEPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP75794.

Entry information

Entry namePFLE_ECOLI
AccessionPrimary (citable) accession number: P75794
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 24, 2009
Last modified: April 16, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene