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P75794

- PFLE_ECOLI

UniProt

P75794 - PFLE_ECOLI

Protein

Putative pyruvate formate-lyase 3-activating enzyme

Gene

ybiY

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 2 (24 Nov 2009)
      Previous versions | rss
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    Functioni

    Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi25 – 251Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi29 – 291Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi32 – 321Iron-sulfur (4Fe-4S-S-AdoMet)By similarity

    GO - Molecular functioni

    1. [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glucose metabolic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciEcoCyc:G6427-MONOMER.
    ECOL316407:JW0808-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative pyruvate formate-lyase 3-activating enzyme (EC:1.97.1.4)
    Alternative name(s):
    Formate-C-acetyltransferase-activating enzyme 3
    PFL-activating enzyme 3
    Gene namesi
    Name:ybiY
    Ordered Locus Names:b0824, JW0808
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13470. ybiY.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 299299Putative pyruvate formate-lyase 3-activating enzymePRO_0000200528Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP75794.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-11444N.
    IntActiP75794. 5 interactions.
    MINTiMINT-1313356.
    STRINGi511145.b0824.

    Structurei

    3D structure databases

    ProteinModelPortaliP75794.
    SMRiP75794. Positions 44-97, 124-254.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1180.
    HOGENOMiHOG000011459.
    KOiK04069.
    OrthoDBiEOG63VBWZ.
    PhylomeDBiP75794.

    Family and domain databases

    InterProiIPR012839. Glycyl_radical_activase.
    IPR001989. Radical_activat_CS.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000371. PFL_act_enz. 1 hit.
    TIGRFAMsiTIGR02494. PFLE_PFLC. 1 hit.
    PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P75794-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIFNIQRYST HDGPGIRTVV FLKGCSLGCR WCQNPESRAR TQDLLYDARL    50
    CLEGCELCAK AAPEVIERAL NGLLIHREKL TPEHLTALTD CCPTQALTVC 100
    GEVKSVEEIM TTVLRDKPFY DRSGGGLTLS GGEPFMQPEM AMALLQASHE 150
    AGIHTAVETC LHVPWKYIAP SLPYIDLFLA DLKHVADAPF KQWTDGNAAR 200
    VLDNLKKLAA AGKKIIIRVP LIQGFNADET SVKAITDFAA DELHVGEIHF 250
    LPYHTLGINK YHLLNLPYDA PEKPLDAPEL LDFAQQYACQ KGLTATLRG 299
    Length:299
    Mass (Da):33,038
    Last modified:November 24, 2009 - v2
    Checksum:iCA8AFB4C956E732C
    GO

    Sequence cautioni

    The sequence BAA35512.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC73911.2.
    AP009048 Genomic DNA. Translation: BAA35512.1. Different initiation.
    PIRiH64819.
    RefSeqiNP_415345.2. NC_000913.3.
    YP_489097.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73911; AAC73911; b0824.
    BAA35512; BAA35512; BAA35512.
    GeneIDi12933841.
    945445.
    KEGGiecj:Y75_p0797.
    eco:b0824.
    PATRICi32116851. VBIEscCol129921_0851.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC73911.2 .
    AP009048 Genomic DNA. Translation: BAA35512.1 . Different initiation.
    PIRi H64819.
    RefSeqi NP_415345.2. NC_000913.3.
    YP_489097.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P75794.
    SMRi P75794. Positions 44-97, 124-254.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-11444N.
    IntActi P75794. 5 interactions.
    MINTi MINT-1313356.
    STRINGi 511145.b0824.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73911 ; AAC73911 ; b0824 .
    BAA35512 ; BAA35512 ; BAA35512 .
    GeneIDi 12933841.
    945445.
    KEGGi ecj:Y75_p0797.
    eco:b0824.
    PATRICi 32116851. VBIEscCol129921_0851.

    Organism-specific databases

    EchoBASEi EB3243.
    EcoGenei EG13470. ybiY.

    Phylogenomic databases

    eggNOGi COG1180.
    HOGENOMi HOG000011459.
    KOi K04069.
    OrthoDBi EOG63VBWZ.
    PhylomeDBi P75794.

    Enzyme and pathway databases

    BioCyci EcoCyc:G6427-MONOMER.
    ECOL316407:JW0808-MONOMER.

    Miscellaneous databases

    PROi P75794.

    Gene expression databases

    Genevestigatori P75794.

    Family and domain databases

    InterProi IPR012839. Glycyl_radical_activase.
    IPR001989. Radical_activat_CS.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000371. PFL_act_enz. 1 hit.
    TIGRFAMsi TIGR02494. PFLE_PFLC. 1 hit.
    PROSITEi PS01087. RADICAL_ACTIVATING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiPFLE_ECOLI
    AccessioniPrimary (citable) accession number: P75794
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 24, 2009
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3