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P75794

- PFLE_ECOLI

UniProt

P75794 - PFLE_ECOLI

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Protein
Putative pyruvate formate-lyase 3-activating enzyme
Gene
ybiY, b0824, JW0808
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine By similarity.

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi25 – 251Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi29 – 291Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi32 – 321Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. glucose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G6427-MONOMER.
ECOL316407:JW0808-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative pyruvate formate-lyase 3-activating enzyme (EC:1.97.1.4)
Alternative name(s):
Formate-C-acetyltransferase-activating enzyme 3
PFL-activating enzyme 3
Gene namesi
Name:ybiY
Ordered Locus Names:b0824, JW0808
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13470. ybiY.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299Putative pyruvate formate-lyase 3-activating enzyme
PRO_0000200528Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP75794.

Interactioni

Protein-protein interaction databases

DIPiDIP-11444N.
IntActiP75794. 5 interactions.
MINTiMINT-1313356.
STRINGi511145.b0824.

Structurei

3D structure databases

ProteinModelPortaliP75794.
SMRiP75794. Positions 44-97, 124-254.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1180.
HOGENOMiHOG000011459.
KOiK04069.
OrthoDBiEOG63VBWZ.
PhylomeDBiP75794.

Family and domain databases

InterProiIPR012839. Glycyl_radical_activase.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF000371. PFL_act_enz. 1 hit.
TIGRFAMsiTIGR02494. PFLE_PFLC. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P75794-1 [UniParc]FASTAAdd to Basket

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MIFNIQRYST HDGPGIRTVV FLKGCSLGCR WCQNPESRAR TQDLLYDARL    50
CLEGCELCAK AAPEVIERAL NGLLIHREKL TPEHLTALTD CCPTQALTVC 100
GEVKSVEEIM TTVLRDKPFY DRSGGGLTLS GGEPFMQPEM AMALLQASHE 150
AGIHTAVETC LHVPWKYIAP SLPYIDLFLA DLKHVADAPF KQWTDGNAAR 200
VLDNLKKLAA AGKKIIIRVP LIQGFNADET SVKAITDFAA DELHVGEIHF 250
LPYHTLGINK YHLLNLPYDA PEKPLDAPEL LDFAQQYACQ KGLTATLRG 299
Length:299
Mass (Da):33,038
Last modified:November 24, 2009 - v2
Checksum:iCA8AFB4C956E732C
GO

Sequence cautioni

The sequence BAA35512.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC73911.2.
AP009048 Genomic DNA. Translation: BAA35512.1. Different initiation.
PIRiH64819.
RefSeqiNP_415345.2. NC_000913.3.
YP_489097.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73911; AAC73911; b0824.
BAA35512; BAA35512; BAA35512.
GeneIDi12933841.
945445.
KEGGiecj:Y75_p0797.
eco:b0824.
PATRICi32116851. VBIEscCol129921_0851.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC73911.2 .
AP009048 Genomic DNA. Translation: BAA35512.1 . Different initiation.
PIRi H64819.
RefSeqi NP_415345.2. NC_000913.3.
YP_489097.1. NC_007779.1.

3D structure databases

ProteinModelPortali P75794.
SMRi P75794. Positions 44-97, 124-254.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-11444N.
IntActi P75794. 5 interactions.
MINTi MINT-1313356.
STRINGi 511145.b0824.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73911 ; AAC73911 ; b0824 .
BAA35512 ; BAA35512 ; BAA35512 .
GeneIDi 12933841.
945445.
KEGGi ecj:Y75_p0797.
eco:b0824.
PATRICi 32116851. VBIEscCol129921_0851.

Organism-specific databases

EchoBASEi EB3243.
EcoGenei EG13470. ybiY.

Phylogenomic databases

eggNOGi COG1180.
HOGENOMi HOG000011459.
KOi K04069.
OrthoDBi EOG63VBWZ.
PhylomeDBi P75794.

Enzyme and pathway databases

BioCyci EcoCyc:G6427-MONOMER.
ECOL316407:JW0808-MONOMER.

Miscellaneous databases

PROi P75794.

Gene expression databases

Genevestigatori P75794.

Family and domain databases

InterProi IPR012839. Glycyl_radical_activase.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view ]
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF000371. PFL_act_enz. 1 hit.
TIGRFAMsi TIGR02494. PFLE_PFLC. 1 hit.
PROSITEi PS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiPFLE_ECOLI
AccessioniPrimary (citable) accession number: P75794
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 24, 2009
Last modified: June 11, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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