P75793 (PFLF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative formate acetyltransferase 3
EC=2.3.1.54

Alternative name(s):
Pyruvate formate-lyase 3

Gene names

Name:	ybiW
Ordered Locus Names:	b0823, JW0807

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	810 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + formate = CoA + pyruvate.
Pathway	Fermentation; pyruvate fermentation; formate from pyruvate: step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 glycine radical domain. Contains 1 PFL domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
PTM	Organic radical
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	formate C-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 810	810	Putative formate acetyltransferase 3		PRO_0000166690
Regions
	Domain	11 – 682	672	PFL
	Domain	689 – 810	122	Glycine radical
Amino acid modifications
	Modified residue	786	1	Glycine radical By similarity

Sequences

	Sequence		Length	Mass (Da)
	P75793 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 8578C59C67F95DE1	FASTA	810	90,125
	10 20 30 40 50 60 MTTLKLDTLS DRIKAHKNAL VHIVKPPVCT ERAQHYTEMY QQHLDKPIPV RRALALAHHL 70 80 90 100 110 120 ANRTIWIKHD ELIIGNQASE VRAAPIFPEY TVSWIEKEID DLADRPGAGF AVSEENKRVL 130 140 150 160 170 180 HEVCPWWRGQ TVQDRCYGMF TDEQKGLLAT GIIKAEGNMT SGDAHLAVNF PLLLEKGLDG 190 200 210 220 230 240 LREEVAERRS RINLTVLEDL HGEQFLKAID IVLVAVSEHI ERFAALAREM AATETRESRR 250 260 270 280 290 300 DELLAMAENC DLIAHQPPQT FWQALQLCYF IQLILQIESN GHSVSFGRMD QYLYPYYRRD 310 320 330 340 350 360 VELNQTLDRE HAIEMLHSCW LKLLEVNKIR SGSHSKASAG SPLYQNVTIG GQNLVDGQPM 370 380 390 400 410 420 DAVNPLSYAI LESCGRLRST QPNLSVRYHA GMSNDFLDAC VQVIRCGFGM PAFNNDEIVI 430 440 450 460 470 480 PEFIKLGIEP QDAYDYAAIG CIETAVGGKW GYRCTGMSFI NFARVMLAAL EGGHDATSGK 490 500 510 520 530 540 VFLPQEKALS AGNFNNFDEV MDAWDTQIRY YTRKSIEIEY VVDTMLEENV HDILCSALVD 550 560 570 580 590 600 DCIERAKSIK QGGAKYDWVS GLQVGIANLG NSLAAVKKLV FEQGAIGQQQ LAAALADDFD 610 620 630 640 650 660 GLTHEQLRQR LINGAPKYGN DDDTVDTLLA RAYQTYIDEL KQYHNPRYGR GPVGGNYYAG 670 680 690 700 710 720 TSSISANVPF GAQTMATPDG RKAHTPLAEG ASPASGTDHL GPTAVIGSVG KLPTAAILGG 730 740 750 760 770 780 VLLNQKLNPA TLENESDKQK LMILLRTFFE VHKGWHIQYN IVSRETLLDA KKHPDQYRDL 790 800 810 VVRVAGYSAF FTALSPDAQD DIIARTEHML

References

[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC73910.1. AP009048 Genomic DNA. Translation: BAA35504.1.
PIR	G64819.
RefSeq	NP_415344.1. NC_000913.2. YP_489096.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P75793.
SMR	P75793. Positions 6-810.
ModBase	Search...
Protein-protein interaction databases
IntAct	P75793. 6 interactions.
STRING	511145.b0823.
Proteomic databases
PaxDb	P75793.
PRIDE	P75793.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73910; AAC73910; b0823. BAA35504; BAA35504; BAA35504.
GeneID	12930984. 945444.
KEGG	ecj:Y75_p0796. eco:b0823.
PATRIC	32116849. VBIEscCol129921_0850.
Organism-specific databases
EchoBASE	EB3112.
EcoGene	EG13328. ybiW.
Phylogenomic databases
eggNOG	COG1882.
HOGENOM	HOG000274400.
KO	K00656.
OMA	HVQYNIV.
ProtClustDB	CLSK880920.
Enzyme and pathway databases
BioCyc	EcoCyc:G6426-MONOMER. ECOL316407:JW0807-MONOMER.
UniPathway	UPA00920; UER00891.
Gene expression databases
Genevestigator	P75793.
Family and domain databases
InterPro	IPR019777. Form_AcTrfase_GR_CS. IPR001150. Gly_radical. IPR010098. PFL2/GDeHydtase_fam. IPR004184. PFL_dom. [Graphical view]
Pfam	PF01228. Gly_radical. 1 hit. PF02901. PFL. 1 hit. [Graphical view]
TIGRFAMs	TIGR01774. PFL2-3. 1 hit.
PROSITE	PS00850. GLY_RADICAL_1. 1 hit. PS51149. GLY_RADICAL_2. 1 hit. PS51554. PFL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PFLF_ECOLI

Accession

Primary (citable) accession number: P75793

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program