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P75793 (PFLF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative formate acetyltransferase 3

EC=2.3.1.54
Alternative name(s):
Pyruvate formate-lyase 3
Gene names
Name:ybiW
Ordered Locus Names:b0823, JW0807
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length810 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Acetyl-CoA + formate = CoA + pyruvate.

Pathway

Fermentation; pyruvate fermentation; formate from pyruvate: step 1/1.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Contains 1 glycine radical domain.

Contains 1 PFL domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionAcyltransferase
Transferase
   PTMOrganic radical
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionformate C-acetyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 810810Putative formate acetyltransferase 3
PRO_0000166690

Regions

Domain11 – 682672PFL
Domain689 – 810122Glycine radical

Amino acid modifications

Modified residue7861Glycine radical By similarity

Sequences

Sequence LengthMass (Da)Tools
P75793 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 8578C59C67F95DE1

FASTA81090,125
        10         20         30         40         50         60 
MTTLKLDTLS DRIKAHKNAL VHIVKPPVCT ERAQHYTEMY QQHLDKPIPV RRALALAHHL 

        70         80         90        100        110        120 
ANRTIWIKHD ELIIGNQASE VRAAPIFPEY TVSWIEKEID DLADRPGAGF AVSEENKRVL 

       130        140        150        160        170        180 
HEVCPWWRGQ TVQDRCYGMF TDEQKGLLAT GIIKAEGNMT SGDAHLAVNF PLLLEKGLDG 

       190        200        210        220        230        240 
LREEVAERRS RINLTVLEDL HGEQFLKAID IVLVAVSEHI ERFAALAREM AATETRESRR 

       250        260        270        280        290        300 
DELLAMAENC DLIAHQPPQT FWQALQLCYF IQLILQIESN GHSVSFGRMD QYLYPYYRRD 

       310        320        330        340        350        360 
VELNQTLDRE HAIEMLHSCW LKLLEVNKIR SGSHSKASAG SPLYQNVTIG GQNLVDGQPM 

       370        380        390        400        410        420 
DAVNPLSYAI LESCGRLRST QPNLSVRYHA GMSNDFLDAC VQVIRCGFGM PAFNNDEIVI 

       430        440        450        460        470        480 
PEFIKLGIEP QDAYDYAAIG CIETAVGGKW GYRCTGMSFI NFARVMLAAL EGGHDATSGK 

       490        500        510        520        530        540 
VFLPQEKALS AGNFNNFDEV MDAWDTQIRY YTRKSIEIEY VVDTMLEENV HDILCSALVD 

       550        560        570        580        590        600 
DCIERAKSIK QGGAKYDWVS GLQVGIANLG NSLAAVKKLV FEQGAIGQQQ LAAALADDFD 

       610        620        630        640        650        660 
GLTHEQLRQR LINGAPKYGN DDDTVDTLLA RAYQTYIDEL KQYHNPRYGR GPVGGNYYAG 

       670        680        690        700        710        720 
TSSISANVPF GAQTMATPDG RKAHTPLAEG ASPASGTDHL GPTAVIGSVG KLPTAAILGG 

       730        740        750        760        770        780 
VLLNQKLNPA TLENESDKQK LMILLRTFFE VHKGWHIQYN IVSRETLLDA KKHPDQYRDL 

       790        800        810 
VVRVAGYSAF FTALSPDAQD DIIARTEHML 

« Hide

References

[1]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC73910.1.
AP009048 Genomic DNA. Translation: BAA35504.1.
PIRG64819.
RefSeqNP_415344.1. NC_000913.3.
YP_489096.1. NC_007779.1.

3D structure databases

ProteinModelPortalP75793.
SMRP75793. Positions 6-810.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP75793. 6 interactions.
STRING511145.b0823.

Proteomic databases

PaxDbP75793.
PRIDEP75793.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73910; AAC73910; b0823.
BAA35504; BAA35504; BAA35504.
GeneID12930984.
945444.
KEGGecj:Y75_p0796.
eco:b0823.
PATRIC32116849. VBIEscCol129921_0850.

Organism-specific databases

EchoBASEEB3112.
EcoGeneEG13328. ybiW.

Phylogenomic databases

eggNOGCOG1882.
HOGENOMHOG000274400.
KOK00656.
OMAENCDIIA.
OrthoDBEOG65TRNB.
PhylomeDBP75793.

Enzyme and pathway databases

BioCycEcoCyc:G6426-MONOMER.
ECOL316407:JW0807-MONOMER.
UniPathwayUPA00920; UER00891.

Gene expression databases

GenevestigatorP75793.

Family and domain databases

InterProIPR019777. Form_AcTrfase_GR_CS.
IPR001150. Gly_radical.
IPR010098. PFL2/GDeHydtase_fam.
IPR004184. PFL_dom.
[Graphical view]
PfamPF01228. Gly_radical. 1 hit.
PF02901. PFL. 1 hit.
[Graphical view]
TIGRFAMsTIGR01774. PFL2-3. 1 hit.
PROSITEPS00850. GLY_RADICAL_1. 1 hit.
PS51149. GLY_RADICAL_2. 1 hit.
PS51554. PFL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP75793.

Entry information

Entry namePFLF_ECOLI
AccessionPrimary (citable) accession number: P75793
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: May 14, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene