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Protein

Phosphoethanolamine transferase OpgE

Gene

opgE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the addition of a phosphoethanolamine moiety to the osmoregulated periplasmic glucan (OPG) backbone.1 Publication

Pathwayi: osmoregulated periplasmic glucan (OPG) biosynthesis

This protein is involved in the pathway osmoregulated periplasmic glucan (OPG) biosynthesis, which is part of Glycan metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway osmoregulated periplasmic glucan (OPG) biosynthesis and in Glycan metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciEcoCyc:G6418-MONOMER.
ECOL316407:JW0800-MONOMER.
MetaCyc:G6418-MONOMER.
UniPathwayiUPA00637.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoethanolamine transferase OpgE (EC:2.7.-.-)
Gene namesi
Name:opgE
Synonyms:ybiP
Ordered Locus Names:b0815, JW0800
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13321. opgE.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333PeriplasmicSequence analysisAdd
BLAST
Transmembranei34 – 5421HelicalSequence analysisAdd
BLAST
Topological domaini55 – 628CytoplasmicSequence analysis
Transmembranei63 – 8321HelicalSequence analysisAdd
BLAST
Topological domaini84 – 10623PeriplasmicSequence analysisAdd
BLAST
Transmembranei107 – 12721HelicalSequence analysisAdd
BLAST
Topological domaini128 – 14619CytoplasmicSequence analysisAdd
BLAST
Transmembranei147 – 16721HelicalSequence analysisAdd
BLAST
Topological domaini168 – 527360PeriplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527Phosphoethanolamine transferase OpgEPRO_0000209145Add
BLAST

Proteomic databases

PaxDbiP75785.
PRIDEiP75785.

Interactioni

Protein-protein interaction databases

BioGridi4259975. 3 interactions.
IntActiP75785. 3 interactions.
STRINGi511145.b0815.

Structurei

3D structure databases

ProteinModelPortaliP75785.
SMRiP75785. Positions 220-502.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108I6E. Bacteria.
COG2194. LUCA.
HOGENOMiHOG000118161.
OMAiRWGKMET.
OrthoDBiEOG6H1PW4.
PhylomeDBiP75785.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.

Sequencei

Sequence statusi: Complete.

P75785-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLTLKESLV TRSRVFSPWT AFYFLQSLLI NLGLGYPFSL LYTAAFTAIL
60 70 80 90 100
LLLWRTLPRV QKVLVGVSSL VAACYFPFAQ AYGAPNFNTL LALHSTNMEE
110 120 130 140 150
STEILTIFPW YSYLVGLFIF ALGVIAIRRK KENEKARWNT FDSLCLVFSV
160 170 180 190 200
ATFFVAPVQN LAWGGVFKLK DTGYPVFRFA KDVIVNNNEV IEEQERMAKL
210 220 230 240 250
SGMKDTWTVT AVKPKYQTYV VVIGESARRD ALGAFGGHWD NTPFASSVNG
260 270 280 290 300
LIFADYIAAS GSTQKSLGLT LNRVVDGKPQ FQDNFVTLAN RAGFQTWWFS
310 320 330 340 350
NQGQIGEYDT AIASIAKRAD EVYFLKEGNF EADKNTKDEA LLDMTAQVLA
360 370 380 390 400
QEHSQPQLIV LHLMGSHPQA CDRTQGKYET FVQSKETSCY LYTMTQTDDL
410 420 430 440 450
LRKLYDQLRN SGSSFSLVYF SDHGLAFKER GKDVQYLAHD DKYQQNFQVP
460 470 480 490 500
FMVISSDDKA HRVIKARRSA NDFLGFFSQW TGIKAKEINI KYPFISEKKA
510 520
GPIYITNFQL QKVDYNHLGT DIFDPKP
Length:527
Mass (Da):59,706
Last modified:February 1, 1997 - v1
Checksum:i7069D6C1A5503A2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73902.1.
AP009048 Genomic DNA. Translation: BAA35487.1.
PIRiG64818.
RefSeqiNP_415336.1. NC_000913.3.
WP_001054678.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73902; AAC73902; b0815.
BAA35487; BAA35487; BAA35487.
GeneIDi945360.
KEGGiecj:JW0800.
eco:b0815.
PATRICi32116833. VBIEscCol129921_0842.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73902.1.
AP009048 Genomic DNA. Translation: BAA35487.1.
PIRiG64818.
RefSeqiNP_415336.1. NC_000913.3.
WP_001054678.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP75785.
SMRiP75785. Positions 220-502.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259975. 3 interactions.
IntActiP75785. 3 interactions.
STRINGi511145.b0815.

Proteomic databases

PaxDbiP75785.
PRIDEiP75785.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73902; AAC73902; b0815.
BAA35487; BAA35487; BAA35487.
GeneIDi945360.
KEGGiecj:JW0800.
eco:b0815.
PATRICi32116833. VBIEscCol129921_0842.

Organism-specific databases

EchoBASEiEB3105.
EcoGeneiEG13321. opgE.

Phylogenomic databases

eggNOGiENOG4108I6E. Bacteria.
COG2194. LUCA.
HOGENOMiHOG000118161.
OMAiRWGKMET.
OrthoDBiEOG6H1PW4.
PhylomeDBiP75785.

Enzyme and pathway databases

UniPathwayiUPA00637.
BioCyciEcoCyc:G6418-MONOMER.
ECOL316407:JW0800-MONOMER.
MetaCyc:G6418-MONOMER.

Miscellaneous databases

PROiP75785.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR000917. Sulfatase_N.
[Graphical view]
PfamiPF00884. Sulfatase. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Biosynthesis of osmoregulated periplasmic glucans in Escherichia coli: the phosphoethanolamine transferase is encoded by opgE."
    Bontemps-Gallo S., Cogez V., Robbe-Masselot C., Quintard K., Dondeyne J., Madec E., Lacroix J.M.
    Biomed. Res. Int. 2013:371429-371429(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PATHWAY, GENE NAME.
    Strain: K12 / ATCC 35607 / JM83.

Entry informationi

Entry nameiOPGE_ECOLI
AccessioniPrimary (citable) accession number: P75785
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 8, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.