Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribosomal RNA large subunit methyltransferase F

Gene

rlmF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically methylates the adenine in position 1618 of 23S rRNA.1 Publication

Miscellaneous

Recombinant protein is able to methylate partially deproteinized 50S ribosomal subunit, but neither the completely assembled 50S subunits nor completely deproteinized 23S rRNA.

Catalytic activityi

S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N6-methyladenine(1618) in 23S rRNA.

GO - Molecular functioni

  • 23S rRNA (adenine(1618)-N(6))-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • rRNA base methylation Source: EcoCyc

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G6416-MONOMER
MetaCyc:G6416-MONOMER
BRENDAi2.1.1.181 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA large subunit methyltransferase F (EC:2.1.1.181)
Alternative name(s):
23S rRNA mA1618 methyltransferase
rRNA adenine N-6-methyltransferase
Gene namesi
Name:rlmF
Synonyms:ybiN
Ordered Locus Names:b0807, JW5107
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13319 rlmF

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002180201 – 308Ribosomal RNA large subunit methyltransferase FAdd BLAST308

Proteomic databases

PaxDbiP75782
PRIDEiP75782

Interactioni

Protein-protein interaction databases

BioGridi4259969, 41 interactors
DIPiDIP-11434N
IntActiP75782, 1 interactor
STRINGi316385.ECDH10B_0875

Structurei

3D structure databases

ProteinModelPortaliP75782
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105EBA Bacteria
COG3129 LUCA
HOGENOMiHOG000218605
InParanoidiP75782
KOiK06970
OMAiTEFHQGH
PhylomeDBiP75782

Family and domain databases

HAMAPiMF_01848 23SrRNA_methyltr_F, 1 hit
InterProiView protein in InterPro
IPR016909 rRNA_lsu_MeTfrase_F
IPR010286 rRNA_lsu_MeTfrase_F-like
IPR029063 SAM-dependent_MTases
PANTHERiPTHR13393 PTHR13393, 1 hit
PfamiView protein in Pfam
PF05971 Methyltransf_10, 1 hit
PIRSFiPIRSF029038 Mtase_YbiN_prd, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

P75782-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAQKPGLHP RNRHHSRYDL ATLCQVNPEL RQFLTLTPAG EQSVDFANPL
60 70 80 90 100
AVKALNKALL AHFYAVANWD IPDGFLCPPV PGRADYIHHL ADLLAEASGT
110 120 130 140 150
IPANASILDI GVGANCIYPL IGVHEYGWRF TGSETSSQAL SSAQAIISSN
160 170 180 190 200
PGLNRAIRLR RQKESGAIFN GIIHKNEQYD ATLCNPPFHD SAAAARAGSE
210 220 230 240 250
RKRRNLGLNK DDALNFGGQQ QELWCEGGEV TFIKKMIEES KGFAKQVMWF
260 270 280 290 300
TSLVSRGENL PPLYRALTDV GAVKVVKKEM AQGQKQSRFI AWTFMNDEQR

RRFVNRQR
Length:308
Mass (Da):34,226
Last modified:December 15, 1998 - v2
Checksum:iE096A6624F37D546
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC73894.2
AP009048 Genomic DNA Translation: BAA35473.2
PIRiG64817
RefSeqiNP_415328.4, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAC73894; AAC73894; b0807
BAA35473; BAA35473; BAA35473
GeneIDi944938
KEGGiecj:JW5107
eco:b0807
PATRICifig|1411691.4.peg.1471

Similar proteinsi

Entry informationi

Entry nameiRLMF_ECOLI
AccessioniPrimary (citable) accession number: P75782
Secondary accession number(s): Q9R3Q9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 15, 1998
Last modified: March 28, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health