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Protein

Ribosomal RNA large subunit methyltransferase F

Gene

rlmF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the adenine in position 1618 of 23S rRNA.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA.

GO - Molecular functioni

  • 23S rRNA (adenine(1618)-N(6))-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • rRNA base methylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G6416-MONOMER.
ECOL316407:JW5107-MONOMER.
MetaCyc:G6416-MONOMER.
BRENDAi2.1.1.181. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA large subunit methyltransferase F (EC:2.1.1.181)
Alternative name(s):
23S rRNA mA1618 methyltransferase
rRNA adenine N-6-methyltransferase
Gene namesi
Name:rlmF
Synonyms:ybiN
Ordered Locus Names:b0807, JW5107
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13319. rlmF.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 308308Ribosomal RNA large subunit methyltransferase FPRO_0000218020Add
BLAST

Proteomic databases

PaxDbiP75782.
PRIDEiP75782.

Interactioni

Protein-protein interaction databases

BioGridi4259969. 7 interactions.
DIPiDIP-11434N.
IntActiP75782. 1 interaction.
MINTiMINT-1267834.
STRINGi511145.b0807.

Structurei

3D structure databases

ProteinModelPortaliP75782.
SMRiP75782. Positions 43-296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105EBA. Bacteria.
COG3129. LUCA.
HOGENOMiHOG000218605.
InParanoidiP75782.
KOiK06970.
OMAiTEFHQGH.
PhylomeDBiP75782.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01848. 23SrRNA_methyltr_F. 1 hit.
InterProiIPR016909. rRNA_lsu_MeTfrase_F.
IPR010286. rRNA_lsu_MeTfrase_F-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13393. PTHR13393. 1 hit.
PfamiPF05971. Methyltransf_10. 1 hit.
[Graphical view]
PIRSFiPIRSF029038. Mtase_YbiN_prd. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

P75782-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAQKPGLHP RNRHHSRYDL ATLCQVNPEL RQFLTLTPAG EQSVDFANPL
60 70 80 90 100
AVKALNKALL AHFYAVANWD IPDGFLCPPV PGRADYIHHL ADLLAEASGT
110 120 130 140 150
IPANASILDI GVGANCIYPL IGVHEYGWRF TGSETSSQAL SSAQAIISSN
160 170 180 190 200
PGLNRAIRLR RQKESGAIFN GIIHKNEQYD ATLCNPPFHD SAAAARAGSE
210 220 230 240 250
RKRRNLGLNK DDALNFGGQQ QELWCEGGEV TFIKKMIEES KGFAKQVMWF
260 270 280 290 300
TSLVSRGENL PPLYRALTDV GAVKVVKKEM AQGQKQSRFI AWTFMNDEQR

RRFVNRQR
Length:308
Mass (Da):34,226
Last modified:December 15, 1998 - v2
Checksum:iE096A6624F37D546
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73894.2.
AP009048 Genomic DNA. Translation: BAA35473.2.
PIRiG64817.
RefSeqiNP_415328.4. NC_000913.3.
WP_001295299.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73894; AAC73894; b0807.
BAA35473; BAA35473; BAA35473.
GeneIDi944938.
KEGGiecj:JW5107.
eco:b0807.
PATRICi32116817. VBIEscCol129921_0834.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73894.2.
AP009048 Genomic DNA. Translation: BAA35473.2.
PIRiG64817.
RefSeqiNP_415328.4. NC_000913.3.
WP_001295299.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP75782.
SMRiP75782. Positions 43-296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259969. 7 interactions.
DIPiDIP-11434N.
IntActiP75782. 1 interaction.
MINTiMINT-1267834.
STRINGi511145.b0807.

Proteomic databases

PaxDbiP75782.
PRIDEiP75782.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73894; AAC73894; b0807.
BAA35473; BAA35473; BAA35473.
GeneIDi944938.
KEGGiecj:JW5107.
eco:b0807.
PATRICi32116817. VBIEscCol129921_0834.

Organism-specific databases

EchoBASEiEB3103.
EcoGeneiEG13319. rlmF.

Phylogenomic databases

eggNOGiENOG4105EBA. Bacteria.
COG3129. LUCA.
HOGENOMiHOG000218605.
InParanoidiP75782.
KOiK06970.
OMAiTEFHQGH.
PhylomeDBiP75782.

Enzyme and pathway databases

BioCyciEcoCyc:G6416-MONOMER.
ECOL316407:JW5107-MONOMER.
MetaCyc:G6416-MONOMER.
BRENDAi2.1.1.181. 2026.

Miscellaneous databases

PROiP75782.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01848. 23SrRNA_methyltr_F. 1 hit.
InterProiIPR016909. rRNA_lsu_MeTfrase_F.
IPR010286. rRNA_lsu_MeTfrase_F-like.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13393. PTHR13393. 1 hit.
PfamiPF05971. Methyltransf_10. 1 hit.
[Graphical view]
PIRSFiPIRSF029038. Mtase_YbiN_prd. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRLMF_ECOLI
AccessioniPrimary (citable) accession number: P75782
Secondary accession number(s): Q9R3Q9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 15, 1998
Last modified: September 7, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Recombinant protein is able to methylate partially deproteinized 50S ribosomal subunit, but neither the completely assembled 50S subunits nor completely deproteinized 23S rRNA.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.