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Protein

Catecholate siderophore receptor Fiu

Gene

fiu

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the active transport across the outer membrane of iron complexed with catecholate siderophores such as dihydroxybenzoylserine and dihydroxybenzoate. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB. Can also transport catechol-substituted cephalosporins. Receptor for microcins M, H47 and E492.5 Publications

GO - Molecular functioni

GO - Biological processi

  • iron ion homeostasis Source: UniProtKB-KW
  • microcin transport Source: EcoliWiki
  • siderophore transport Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciEcoCyc:G6414-MONOMER.
ECOL316407:JW0790-MONOMER.

Protein family/group databases

TCDBi1.B.14.1.9. the outer membrane receptor (omr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Catecholate siderophore receptor Fiu
Alternative name(s):
Ferric iron uptake protein
TonB-dependent receptor Fiu
Gene namesi
Name:fiu
Synonyms:ybiL
Ordered Locus Names:b0805, JW0790
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13317. fiu.

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence analysisAdd
BLAST
Chaini32 – 760729Catecholate siderophore receptor FiuPRO_0000034790Add
BLAST

Proteomic databases

PaxDbiP75780.
PRIDEiP75780.

Expressioni

Inductioni

Repressed by iron.3 Publications

Interactioni

Protein-protein interaction databases

BioGridi4259972. 121 interactions.
DIPiDIP-11432N.
IntActiP75780. 3 interactions.
STRINGi511145.b0805.

Structurei

3D structure databases

ProteinModelPortaliP75780.
SMRiP75780. Positions 42-760.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi743 – 76018TonB C-terminal boxAdd
BLAST

Sequence similaritiesi

Belongs to the TonB-dependent receptor family.Curated

Keywords - Domaini

Signal, TonB box, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4108IU3. Bacteria.
COG4774. LUCA.
HOGENOMiHOG000130432.
InParanoidiP75780.
KOiK16090.
OMAiQQVIKDT.
OrthoDBiEOG6JQGXR.
PhylomeDBiP75780.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProiIPR030148. Fiu.
IPR012910. Plug_dom.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010105. TonB_sidphr_rcpt.
[Graphical view]
PANTHERiPTHR32552:SF8. PTHR32552:SF8. 2 hits.
PfamiPF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01783. TonB-siderophor. 1 hit.
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P75780-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENNRNFPAR QFHSLTFFAG LCIGITPVAQ ALAAEGQTNA DDTLVVEAST
60 70 80 90 100
PSLYAPQQSA DPKFSRPVAD TTRTMTVISE QVIKDQGATN LTDALKNVPG
110 120 130 140 150
VGAFFAGENG NSTTGDAIYM RGADTSNSIY IDGIRDIGSV SRDTFNTEQV
160 170 180 190 200
EVIKGPSGTD YGRSAPTGSI NMISKQPRND SGIDASASIG SAWFRRGTLD
210 220 230 240 250
VNQVIGDTTA VRLNVMGEKT HDAGRDKVKN ERYGVAPSVA FGLGTANRLY
260 270 280 290 300
LNYLHVTQHN TPDGGIPTIG LPGYSAPSAG TAALNHSGKV DTHNFYGTDS
310 320 330 340 350
DYDDSTTDTA TMRFEHDIND NTTIRNTTRW SRVKQDYLMT AIMGGASNIT
360 370 380 390 400
QPTSDVNSWT WSRTANTKDV SNKILTNQTN LTSTFYTGSI GHDVSTGVEF
410 420 430 440 450
TRETQTNYGV NPVTLPAVNI YHPDSSIHPG GLTRNGANAN GQTDTFAIYA
460 470 480 490 500
FDTLQITRDF ELNGGIRLDN YHTEYDSATA CGGSGRGAIT CPTGVAKGSP
510 520 530 540 550
VTTVDTAKSG NLMNWKAGAL YHLTENGNVY INYAVSQQPP GGNNFALAQS
560 570 580 590 600
GSGNSANRTD FKPQKANTSE IGTKWQVLDK RLLLTAALFR TDIENEVEQN
610 620 630 640 650
DDGTYSQYGK KRVEGYEISV AGNITPAWQV IGGYTQQKAT IKNGKDVAQD
660 670 680 690 700
GSSSLPYTPE HAFTLWSQYQ ATDDISVGAG ARYIGSMHKG SDGAVGTPAF
710 720 730 740 750
TEGYWVADAK LGYRVNRNLD FQLNVYNLFD TDYVASINKS GYRYHPGEPR
760
TFLLTANMHF
Length:760
Mass (Da):81,960
Last modified:February 1, 1997 - v1
Checksum:i1B66B9C2EF68C7CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73892.1.
AP009048 Genomic DNA. Translation: BAA35471.1.
PIRiE64817.
RefSeqiNP_415326.1. NC_000913.3.
WP_000430057.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73892; AAC73892; b0805.
BAA35471; BAA35471; BAA35471.
GeneIDi946246.
KEGGiecj:JW0790.
eco:b0805.
PATRICi32116813. VBIEscCol129921_0832.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73892.1.
AP009048 Genomic DNA. Translation: BAA35471.1.
PIRiE64817.
RefSeqiNP_415326.1. NC_000913.3.
WP_000430057.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP75780.
SMRiP75780. Positions 42-760.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259972. 121 interactions.
DIPiDIP-11432N.
IntActiP75780. 3 interactions.
STRINGi511145.b0805.

Protein family/group databases

TCDBi1.B.14.1.9. the outer membrane receptor (omr) family.

Proteomic databases

PaxDbiP75780.
PRIDEiP75780.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73892; AAC73892; b0805.
BAA35471; BAA35471; BAA35471.
GeneIDi946246.
KEGGiecj:JW0790.
eco:b0805.
PATRICi32116813. VBIEscCol129921_0832.

Organism-specific databases

EchoBASEiEB3101.
EcoGeneiEG13317. fiu.

Phylogenomic databases

eggNOGiENOG4108IU3. Bacteria.
COG4774. LUCA.
HOGENOMiHOG000130432.
InParanoidiP75780.
KOiK16090.
OMAiQQVIKDT.
OrthoDBiEOG6JQGXR.
PhylomeDBiP75780.

Enzyme and pathway databases

BioCyciEcoCyc:G6414-MONOMER.
ECOL316407:JW0790-MONOMER.

Miscellaneous databases

PROiP75780.

Family and domain databases

Gene3Di2.170.130.10. 1 hit.
2.40.170.20. 1 hit.
InterProiIPR030148. Fiu.
IPR012910. Plug_dom.
IPR000531. TonB-dep_rcpt_b-brl.
IPR010916. TonB_box_CS.
IPR010105. TonB_sidphr_rcpt.
[Graphical view]
PANTHERiPTHR32552:SF8. PTHR32552:SF8. 2 hits.
PfamiPF07715. Plug. 1 hit.
PF00593. TonB_dep_Rec. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01783. TonB-siderophor. 1 hit.
PROSITEiPS00430. TONB_DEPENDENT_REC_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Iron-regulated outer membrane proteins of Escherichia coli K-12 and mechanism of action of catechol-substituted cephalosporins."
    Curtis N.A.C., Eisenstadt R.L., East S.J., Cornford R.J., Walker L.A., White A.J.
    Antimicrob. Agents Chemother. 32:1879-1886(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TRANSPORT OF CATECHOL-SUBSTITUTED CEPHALOSPORINS, INDUCTION.
    Strain: K12.
  5. "Dihydroxybenzoylserine -- a siderophore for E. coli."
    Hantke K.
    FEMS Microbiol. Lett. 55:5-8(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: K12.
  6. "Cir and Fiu proteins in the outer membrane of Escherichia coli catalyze transport of monomeric catechols: study with beta-lactam antibiotics containing catechol and analogous groups."
    Nikaido H., Rosenberg E.Y.
    J. Bacteriol. 172:1361-1367(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TRANSPORT OF CATECHOL-CONTAINING ANTIBIOTICS.
    Strain: K12.
  7. "Iron content and FNR-dependent gene regulation in Escherichia coli."
    Niehaus F., Hantke K., Unden G.
    FEMS Microbiol. Lett. 68:319-323(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  8. "Differential fiu-lacZ fusion regulation linked to Escherichia coli colony development."
    Newman D.L., Shapiro J.A.
    Mol. Microbiol. 33:18-32(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY, PUTATIVE FUNCTION.
  10. "The colicin G, H and X determinants encode microcins M and H47, which might utilize the catecholate siderophore receptors FepA, Cir, Fiu and IroN."
    Patzer S.I., Baquero M.R., Bravo D., Moreno F., Hantke K.
    Microbiology 149:2557-2570(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Parasitism of iron-siderophore receptors of Escherichia coli by the siderophore-peptide microcin E492m and its unmodified counterpart."
    Destoumieux-Garzon D., Peduzzi J., Thomas X., Djediat C., Rebuffat S.
    BioMetals 19:181-191(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiFIU_ECOLI
AccessioniPrimary (citable) accession number: P75780
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 1, 1997
Last modified: July 6, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.