P75780 (FIU_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catecholate siderophore receptor Fiu

Alternative name(s):
Ferric iron uptake protein
TonB-dependent receptor Fiu

Gene names

Name:	fiu
Synonyms:	ybiL
Ordered Locus Names:	b0805, JW0790

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	760 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the active transport across the outer membrane of iron complexed with catecholate siderophores such as dihydroxybenzoylserine and dihydroxybenzoate. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB. Can also transport catechol-substitued cephalosporins. Receptor for microcins M, H47 and E492. Ref.4 Ref.5 Ref.6 Ref.9 Ref.10 Ref.11
Subcellular location	Cell outer membrane.
Induction	Repressed by iron. Ref.4 Ref.7 Ref.8
Sequence similarities	Belongs to the TonB-dependent receptor family.

Ontologies

Keywords
Biological process	Ion transport Iron transport Transport
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Signal TonB box Transmembrane Transmembrane beta strand
Ligand	Iron
Molecular function	Receptor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	microcin transport Inferred from mutant phenotype PubMed 15980406. Source: EcoliWiki siderophore transmembrane transport Inferred from electronic annotation. Source: GOC
Cellular_component	cell outer membrane Inferred from direct assay Ref.9. Source: EcoCyc integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	iron ion binding Inferred from electronic annotation. Source: InterPro receptor activity Inferred from electronic annotation. Source: InterPro siderophore transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 760	729	Catecholate siderophore receptor Fiu		PRO_0000034790
Regions
	Motif	743 – 760	18	TonB C-terminal box

Sequences

	Sequence		Length	Mass (Da)
	P75780 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 1B66B9C2EF68C7CA	FASTA	760	81,960
	10 20 30 40 50 60 MENNRNFPAR QFHSLTFFAG LCIGITPVAQ ALAAEGQTNA DDTLVVEAST PSLYAPQQSA 70 80 90 100 110 120 DPKFSRPVAD TTRTMTVISE QVIKDQGATN LTDALKNVPG VGAFFAGENG NSTTGDAIYM 130 140 150 160 170 180 RGADTSNSIY IDGIRDIGSV SRDTFNTEQV EVIKGPSGTD YGRSAPTGSI NMISKQPRND 190 200 210 220 230 240 SGIDASASIG SAWFRRGTLD VNQVIGDTTA VRLNVMGEKT HDAGRDKVKN ERYGVAPSVA 250 260 270 280 290 300 FGLGTANRLY LNYLHVTQHN TPDGGIPTIG LPGYSAPSAG TAALNHSGKV DTHNFYGTDS 310 320 330 340 350 360 DYDDSTTDTA TMRFEHDIND NTTIRNTTRW SRVKQDYLMT AIMGGASNIT QPTSDVNSWT 370 380 390 400 410 420 WSRTANTKDV SNKILTNQTN LTSTFYTGSI GHDVSTGVEF TRETQTNYGV NPVTLPAVNI 430 440 450 460 470 480 YHPDSSIHPG GLTRNGANAN GQTDTFAIYA FDTLQITRDF ELNGGIRLDN YHTEYDSATA 490 500 510 520 530 540 CGGSGRGAIT CPTGVAKGSP VTTVDTAKSG NLMNWKAGAL YHLTENGNVY INYAVSQQPP 550 560 570 580 590 600 GGNNFALAQS GSGNSANRTD FKPQKANTSE IGTKWQVLDK RLLLTAALFR TDIENEVEQN 610 620 630 640 650 660 DDGTYSQYGK KRVEGYEISV AGNITPAWQV IGGYTQQKAT IKNGKDVAQD GSSSLPYTPE 670 680 690 700 710 720 HAFTLWSQYQ ATDDISVGAG ARYIGSMHKG SDGAVGTPAF TEGYWVADAK LGYRVNRNLD 730 740 750 760 FQLNVYNLFD TDYVASINKS GYRYHPGEPR TFLLTANMHF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Iron-regulated outer membrane proteins of Escherichia coli K-12 and mechanism of action of catechol-substituted cephalosporins." Curtis N.A.C., Eisenstadt R.L., East S.J., Cornford R.J., Walker L.A., White A.J. Antimicrob. Agents Chemother. 32:1879-1886(1988) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN TRANSPORT OF CATECHOL-SUBSTITUED CEPHALOSPORINS, INDUCTION. Strain: K12.
[5]	"Dihydroxybenzoylserine -- a siderophore for E. coli." Hantke K. FEMS Microbiol. Lett. 55:5-8(1990) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: K12.
[6]	"Cir and Fiu proteins in the outer membrane of Escherichia coli catalyze transport of monomeric catechols: study with beta-lactam antibiotics containing catechol and analogous groups." Nikaido H., Rosenberg E.Y. J. Bacteriol. 172:1361-1367(1990) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN TRANSPORT OF CATECHOL-CONTAINING ANTIBIOTICS. Strain: K12.
[7]	"Iron content and FNR-dependent gene regulation in Escherichia coli." Niehaus F., Hantke K., Unden G. FEMS Microbiol. Lett. 68:319-323(1991) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[8]	"Differential fiu-lacZ fusion regulation linked to Escherichia coli colony development." Newman D.L., Shapiro J.A. Mol. Microbiol. 33:18-32(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION. Strain: K12.
[9]	"Proteomic analysis of the Escherichia coli outer membrane." Molloy M.P., Herbert B.R., Slade M.B., Rabilloud T., Nouwens A.S., Williams K.L., Gooley A.A. Eur. J. Biochem. 267:2871-2881(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, PUTATIVE FUNCTION.
[10]	"The colicin G, H and X determinants encode microcins M and H47, which might utilize the catecholate siderophore receptors FepA, Cir, Fiu and IroN." Patzer S.I., Baquero M.R., Bravo D., Moreno F., Hantke K. Microbiology 149:2557-2570(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[11]	"Parasitism of iron-siderophore receptors of Escherichia coli by the siderophore-peptide microcin E492m and its unmodified counterpart." Destoumieux-Garzon D., Peduzzi J., Thomas X., Djediat C., Rebuffat S. BioMetals 19:181-191(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC73892.1. AP009048 Genomic DNA. Translation: BAA35471.1.
PIR	E64817.
RefSeq	NP_415326.1. NC_000913.2. YP_489078.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P75780.
SMR	P75780. Positions 42-760.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11432N.
IntAct	P75780. 3 interactions.
STRING	511145.b0805.
Protein family/group databases
TCDB	1.B.14.1.11. outer membrane receptor (OMR) family.
Proteomic databases
PaxDb	P75780.
PRIDE	P75780.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73892; AAC73892; b0805. BAA35471; BAA35471; BAA35471.
GeneID	12930978. 946246.
KEGG	ecj:Y75_p0778. eco:b0805.
PATRIC	32116813. VBIEscCol129921_0832.
Organism-specific databases
EchoBASE	EB3101.
EcoGene	EG13317. fiu.
Phylogenomic databases
eggNOG	COG4774.
HOGENOM	HOG000130432.
KO	K16090.
OMA	YTGAIGH.
ProtClustDB	PRK09840.
Enzyme and pathway databases
BioCyc	EcoCyc:G6414-MONOMER. ECOL316407:JW0790-MONOMER.
Gene expression databases
Genevestigator	P75780.
Family and domain databases
Gene3D	2.170.130.10. 1 hit. 2.40.170.20. 1 hit.
InterPro	IPR012910. Plug. IPR000531. TonB-dep_rcpt_b-brl. IPR010916. TonB_box_CS. IPR010105. TonB_sidphr_rcpt. [Graphical view]
Pfam	PF07715. Plug. 1 hit. PF00593. TonB_dep_Rec. 1 hit. [Graphical view]
TIGRFAMs	TIGR01783. TonB-siderophor. 1 hit.
PROSITE	PS00430. TONB_DEPENDENT_REC_1. 1 hit. PS01156. TONB_DEPENDENT_REC_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FIU_ECOLI

Accession

Primary (citable) accession number: P75780

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program