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Protein

Esterase YbfF

Gene

ybfF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891By similarity
Active sitei234 – 2341By similarity

GO - Molecular functioni

  • carboxylic ester hydrolase activity Source: EcoCyc
  • thiolester hydrolase activity Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Enzyme and pathway databases

BioCyciEcoCyc:EG11776-MONOMER.
ECOL316407:JW0673-MONOMER.
BRENDAi3.1.2.2. 2026.

Protein family/group databases

ESTHERiecoli-ybff. 6_AlphaBeta_hydrolase.

Names & Taxonomyi

Protein namesi
Recommended name:
Esterase YbfF (EC:3.1.-.-)
Gene namesi
Name:ybfF
Ordered Locus Names:b0686, JW0673
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11776. ybfF.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 254254Esterase YbfFPRO_0000207081Add
BLAST

Proteomic databases

EPDiP75736.
PaxDbiP75736.
PRIDEiP75736.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
groLP0A6F51EBI-1119842,EBI-543750

Protein-protein interaction databases

BioGridi4261906. 3 interactions.
IntActiP75736. 4 interactions.
STRINGi511145.b0686.

Structurei

Secondary structure

1
254
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 84Combined sources
Beta strandi19 – 224Combined sources
Turni29 – 324Combined sources
Helixi33 – 397Combined sources
Turni40 – 423Combined sources
Beta strandi45 – 484Combined sources
Helixi64 – 7815Combined sources
Beta strandi83 – 886Combined sources
Helixi90 – 10112Combined sources
Helixi103 – 1053Combined sources
Beta strandi106 – 1138Combined sources
Helixi125 – 13612Combined sources
Helixi142 – 1498Combined sources
Turni150 – 1523Combined sources
Helixi156 – 1638Combined sources
Beta strandi170 – 1734Combined sources
Helixi175 – 1806Combined sources
Helixi182 – 1865Combined sources
Beta strandi198 – 2014Combined sources
Helixi211 – 2133Combined sources
Helixi214 – 2207Combined sources
Beta strandi224 – 2263Combined sources
Helixi236 – 2394Combined sources
Helixi241 – 25313Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3BF7X-ray1.10A/B1-254[»]
3BF8X-ray1.68A/B1-254[»]
ProteinModelPortaliP75736.
SMRiP75736. Positions 1-254.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP75736.

Family & Domainsi

Sequence similaritiesi

Belongs to the DmpD/TodF/XylF esterase family.Curated

Phylogenomic databases

eggNOGiENOG4105EV9. Bacteria.
COG0596. LUCA.
HOGENOMiHOG000028067.
InParanoidiP75736.
KOiK01175.
OMAiASHWIHA.
OrthoDBiEOG6PZXDZ.
PhylomeDBiP75736.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

P75736-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLNIRAQTA QNQHNNSPIV LVHGLFGSLD NLGVLARDLV NDHNIIQVDM
60 70 80 90 100
RNHGLSPRDP VMNYPAMAQD LVDTLDAQQI DKATFIGHSM GGKAVMALTA
110 120 130 140 150
LASDRIDKLV AIDIAPVDYH VRRHDEIFAA INAVSESDAQ TRQQAAAIMR
160 170 180 190 200
QHLNEEGVIQ FLLKSFVDGE WRFNVPVLWD QYPHIVGWEK IPAWDHPALF
210 220 230 240 250
IPGGNSPYVS EQYRDDLLAQ FPQARAHVIA GAGHWVHAEK PDAVLRAIRR

YLND
Length:254
Mass (Da):28,437
Last modified:February 1, 1997 - v1
Checksum:i8B2F6E28713D9B8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73780.1.
AP009048 Genomic DNA. Translation: BAA35335.1.
PIRiE64803.
RefSeqiNP_415212.1. NC_000913.3.
WP_000773288.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73780; AAC73780; b0686.
BAA35335; BAA35335; BAA35335.
GeneIDi945288.
KEGGiecj:JW0673.
eco:b0686.
PATRICi32116565. VBIEscCol129921_0715.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73780.1.
AP009048 Genomic DNA. Translation: BAA35335.1.
PIRiE64803.
RefSeqiNP_415212.1. NC_000913.3.
WP_000773288.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3BF7X-ray1.10A/B1-254[»]
3BF8X-ray1.68A/B1-254[»]
ProteinModelPortaliP75736.
SMRiP75736. Positions 1-254.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261906. 3 interactions.
IntActiP75736. 4 interactions.
STRINGi511145.b0686.

Protein family/group databases

ESTHERiecoli-ybff. 6_AlphaBeta_hydrolase.

Proteomic databases

EPDiP75736.
PaxDbiP75736.
PRIDEiP75736.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73780; AAC73780; b0686.
BAA35335; BAA35335; BAA35335.
GeneIDi945288.
KEGGiecj:JW0673.
eco:b0686.
PATRICi32116565. VBIEscCol129921_0715.

Organism-specific databases

EchoBASEiEB1725.
EcoGeneiEG11776. ybfF.

Phylogenomic databases

eggNOGiENOG4105EV9. Bacteria.
COG0596. LUCA.
HOGENOMiHOG000028067.
InParanoidiP75736.
KOiK01175.
OMAiASHWIHA.
OrthoDBiEOG6PZXDZ.
PhylomeDBiP75736.

Enzyme and pathway databases

BioCyciEcoCyc:EG11776-MONOMER.
ECOL316407:JW0673-MONOMER.
BRENDAi3.1.2.2. 2026.

Miscellaneous databases

EvolutionaryTraceiP75736.
PROiP75736.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
[Graphical view]
PfamiPF12697. Abhydrolase_6. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography."
    Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B.
    Electrophoresis 20:2181-2195(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: B / BL21.
  5. "Enzyme genomics: application of general enzymatic screens to discover new enzymes."
    Kuznetsova E., Proudfoot M., Sanders S.A., Reinking J., Savchenko A., Arrowsmith C.H., Edwards A.M., Yakunin A.F.
    FEMS Microbiol. Rev. 29:263-279(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiYBFF_ECOLI
AccessioniPrimary (citable) accession number: P75736
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 16, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.