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Protein

Chitoporin

Gene

chiP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the uptake of chitosugars.2 Publications

GO - Molecular functioni

GO - Biological processi

  • diacetylchitobiose metabolic process Source: EcoCyc
  • ion transport Source: UniProtKB-KW
  • polysaccharide transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Porin

Keywords - Biological processi

Ion transport, Polysaccharide transport, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G6370-MONOMER.
ECOL316407:JW0667-MONOMER.
MetaCyc:G6370-MONOMER.

Protein family/group databases

TCDBi1.B.25.1.13. the outer membrane porin (opr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitoporin
Alternative name(s):
ChiP-III
Gene namesi
Name:chiP
Synonyms:ybfM
Ordered Locus Names:b0681, JW0667
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13659. chiP.

Subcellular locationi

  • Cell outer membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence analysisAdd
BLAST
Chaini33 – 468436ChitoporinPRO_0000168697Add
BLAST

Proteomic databases

PaxDbiP75733.

Expressioni

Inductioni

In the absence of chitobiose, expression of chiPQ is silenced by the MicM small regulatory RNA (sRNA), which sequesters the ribosome binding site of the chiPQ mRNA by an antisense mechanism. In the presence of chitosugars, the chbBCARFG chitobiose operon is induced and acts as an RNA trap to degrade the constitutively expressed MicM, leading to the translation of chiPQ.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4261649. 315 interactions.
STRINGi511145.b0681.

Structurei

3D structure databases

ProteinModelPortaliP75733.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

HOGENOMiHOG000273273.
InParanoidiP75733.
OMAiTWVKAEG.
OrthoDBiEOG6CP3RN.
PhylomeDBiP75733.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR005318. OM_porin_bac.
IPR023614. Porin_dom.
[Graphical view]
PfamiPF03573. OprD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P75733-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTFSGKRST LALAIAGVTA MSGFMAMPEA RAEGFIDDST LTGGIYYWQR
60 70 80 90 100
ERDRKDVTDG DKYKTNLSHS TWNANLDFQS GYAADMFGLD IAAFTAIEMA
110 120 130 140 150
ENGDSSHPNE IAFSKSNKAY DEDWSGDKSG ISLYKAAAKF KYGPVWARAG
160 170 180 190 200
YIQPTGQTLL APHWSFMPGT YQGAEAGANF DYGDAGALSF SYMWTNEYKA
210 220 230 240 250
PWHLEMDEFY QNDKTTKVDY LHSFGAKYDF KNNFVLEAAF GQAEGYIDQY
260 270 280 290 300
FAKASYKFDI AGSPLTTSYQ FYGTRDKVDD RSVNDLYDGT AWLQALTFGY
310 320 330 340 350
RAADVVDLRL EGTWVKADGQ QGYFLQRMTP TYASSNGRLD IWWDNRSDFN
360 370 380 390 400
ANGEKAVFFG AMYDLKNWNL PGFAIGASYV YAWDAKPATW QSNPDAYYDK
410 420 430 440 450
NRTIEESAYS LDAVYTIQDG RAKGTMFKLH FTEYDNHSDI PSWGGGYGNI
460
FQDERDVKFM VIAPFTIF
Length:468
Mass (Da):52,780
Last modified:February 1, 1997 - v1
Checksum:i954B5A778AE1C2E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73775.1.
AP009048 Genomic DNA. Translation: BAA35329.2.
PIRiH64802.
RefSeqiNP_415207.1. NC_000913.3.
WP_001258819.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73775; AAC73775; b0681.
BAA35329; BAA35329; BAA35329.
GeneIDi945296.
KEGGiecj:JW0667.
eco:b0681.
PATRICi32116555. VBIEscCol129921_0710.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73775.1.
AP009048 Genomic DNA. Translation: BAA35329.2.
PIRiH64802.
RefSeqiNP_415207.1. NC_000913.3.
WP_001258819.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP75733.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261649. 315 interactions.
STRINGi511145.b0681.

Protein family/group databases

TCDBi1.B.25.1.13. the outer membrane porin (opr) family.

Proteomic databases

PaxDbiP75733.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73775; AAC73775; b0681.
BAA35329; BAA35329; BAA35329.
GeneIDi945296.
KEGGiecj:JW0667.
eco:b0681.
PATRICi32116555. VBIEscCol129921_0710.

Organism-specific databases

EchoBASEiEB3423.
EcoGeneiEG13659. chiP.

Phylogenomic databases

HOGENOMiHOG000273273.
InParanoidiP75733.
OMAiTWVKAEG.
OrthoDBiEOG6CP3RN.
PhylomeDBiP75733.

Enzyme and pathway databases

BioCyciEcoCyc:G6370-MONOMER.
ECOL316407:JW0667-MONOMER.
MetaCyc:G6370-MONOMER.

Miscellaneous databases

PROiP75733.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR005318. OM_porin_bac.
IPR023614. Porin_dom.
[Graphical view]
PfamiPF03573. OprD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "The beta-barrel finder (BBF) program, allowing identification of outer membrane beta-barrel proteins encoded within prokaryotic genomes."
    Zhai Y., Saier M.H. Jr.
    Protein Sci. 11:2196-2207(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, SIMILARITY.
  5. "Comparative genomics and experimental characterization of N-acetylglucosamine utilization pathway of Shewanella oneidensis."
    Yang C., Rodionov D.A., Li X., Laikova O.N., Gelfand M.S., Zagnitko O.P., Romine M.F., Obraztsova A.Y., Nealson K.H., Osterman A.L.
    J. Biol. Chem. 281:29872-29885(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, GENE NAME.
  6. "A conserved small RNA promotes silencing of the outer membrane protein YbfM."
    Rasmussen A.A., Johansen J., Nielsen J.S., Overgaard M., Kallipolitis B., Valentin-Hansen P.
    Mol. Microbiol. 72:566-577(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12.
  7. Cited for: FUNCTION IN CHITOBIOSE UTILIZATION, INDUCTION.
    Strain: K12.

Entry informationi

Entry nameiCHIP_ECOLI
AccessioniPrimary (citable) accession number: P75733
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: April 13, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.