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Protein

2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase

Gene

ubiF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinol.

Cofactori

FADCurated

Pathwayi: ubiquinone biosynthesis

This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • secondary metabolite biosynthetic process Source: GO_Central
  • ubiquinone biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Ubiquinone biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciEcoCyc:OCTAPRENYL-METHYL-METHOXY-BENZOQ-OH-MON.
ECOL316407:JW0659-MONOMER.
MetaCyc:OCTAPRENYL-METHYL-METHOXY-BENZOQ-OH-MON.
BRENDAi1.14.99.B4. 2026.
UniPathwayiUPA00232.

Names & Taxonomyi

Protein namesi
Recommended name:
2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (EC:1.14.13.-)
Gene namesi
Name:ubiF
Synonyms:yleB
Ordered Locus Names:b0662, JW0659
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13658. ubiF.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3913912-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylasePRO_0000207587Add
BLAST

Proteomic databases

PaxDbiP75728.
PRIDEiP75728.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
insO1P756803EBI-562588,EBI-9152908

Protein-protein interaction databases

BioGridi4261231. 299 interactions.
DIPiDIP-11069N.
IntActiP75728. 12 interactions.
STRINGi511145.b0662.

Structurei

3D structure databases

ProteinModelPortaliP75728.
SMRiP75728. Positions 6-39, 156-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UbiH/COQ6 family.Curated

Phylogenomic databases

eggNOGiENOG4105EAK. Bacteria.
COG0654. LUCA.
HOGENOMiHOG000255771.
InParanoidiP75728.
KOiK03184.
OMAiTWEIEGF.
PhylomeDBiP75728.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR002938. FAD-bd.
IPR023753. FAD/NAD-binding_dom.
IPR018168. Ubi_Hdrlase_CS.
IPR010971. UbiH/COQ6.
[Graphical view]
PfamiPF01494. FAD_binding_3. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR01988. Ubi-OHases. 1 hit.
PROSITEiPS01304. UBIH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P75728-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNQPTEIAI VGGGMVGGAL ALGLAQHGFA VTVIEHAEPA PFVADSQPDV
60 70 80 90 100
RISAISAASV SLLKGLGVWD AVQAMRCHPY RRLETWEWET AHVVFDAAEL
110 120 130 140 150
KLPLLGYMVE NTVLQQALWQ ALEAHPKVTL RVPGSLIALH RHDDLQELEL
160 170 180 190 200
KGGEVIRAKL VIGADGANSQ VRQMAGIGVH AWQYAQSCML ISVQCENDPG
210 220 230 240 250
DSTWQQFTPD GPRAFLPLFD NWASLVWYDS PARIRQLQNM NMAQLQAEIA
260 270 280 290 300
KHFPSRLGYV TPLAAGAFPL TRRHALQYVQ PGLALVGDAA HTIHPLAGQG
310 320 330 340 350
VNLGYRDVDA LIDVLVNARS YGEAWASYPV LKRYQMRRMA DNFIMQSGMD
360 370 380 390
LFYAGFSNNL PPLRFMRNLG LMAAERAGVL KRQALKYALG L
Length:391
Mass (Da):42,953
Last modified:February 1, 1997 - v1
Checksum:i05325684AAB6D30A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73763.1.
AP009048 Genomic DNA. Translation: BAA35316.1.
U82598 Genomic DNA. Translation: AAB40864.1.
PIRiD64801.
RefSeqiNP_415195.1. NC_000913.3.
WP_000184541.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73763; AAC73763; b0662.
BAA35316; BAA35316; BAA35316.
GeneIDi945261.
KEGGiecj:JW0659.
eco:b0662.
PATRICi32116513. VBIEscCol129921_0696.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73763.1.
AP009048 Genomic DNA. Translation: BAA35316.1.
U82598 Genomic DNA. Translation: AAB40864.1.
PIRiD64801.
RefSeqiNP_415195.1. NC_000913.3.
WP_000184541.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP75728.
SMRiP75728. Positions 6-39, 156-338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261231. 299 interactions.
DIPiDIP-11069N.
IntActiP75728. 12 interactions.
STRINGi511145.b0662.

Proteomic databases

PaxDbiP75728.
PRIDEiP75728.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73763; AAC73763; b0662.
BAA35316; BAA35316; BAA35316.
GeneIDi945261.
KEGGiecj:JW0659.
eco:b0662.
PATRICi32116513. VBIEscCol129921_0696.

Organism-specific databases

EchoBASEiEB3422.
EcoGeneiEG13658. ubiF.

Phylogenomic databases

eggNOGiENOG4105EAK. Bacteria.
COG0654. LUCA.
HOGENOMiHOG000255771.
InParanoidiP75728.
KOiK03184.
OMAiTWEIEGF.
PhylomeDBiP75728.

Enzyme and pathway databases

UniPathwayiUPA00232.
BioCyciEcoCyc:OCTAPRENYL-METHYL-METHOXY-BENZOQ-OH-MON.
ECOL316407:JW0659-MONOMER.
MetaCyc:OCTAPRENYL-METHYL-METHOXY-BENZOQ-OH-MON.
BRENDAi1.14.99.B4. 2026.

Miscellaneous databases

PROiP75728.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR002938. FAD-bd.
IPR023753. FAD/NAD-binding_dom.
IPR018168. Ubi_Hdrlase_CS.
IPR010971. UbiH/COQ6.
[Graphical view]
PfamiPF01494. FAD_binding_3. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR01988. Ubi-OHases. 1 hit.
PROSITEiPS01304. UBIH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBIF_ECOLI
AccessioniPrimary (citable) accession number: P75728
Secondary accession number(s): P77111
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 1, 1997
Last modified: September 7, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.