P75726 (CILA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Citrate lyase alpha chain
Short name=Citrase alpha chain
EC=4.1.3.6

Alternative name(s):
Citrate (pro-3S)-lyase alpha chain
Citrate CoA-transferase subunit
EC=2.8.3.10

Gene names

Name:	citF
Synonyms:	ybdV
Ordered Locus Names:	b0615, JW5087

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Represents a citrate:acetyl-ACP transferase By similarity.
Catalytic activity	Citrate = acetate + oxaloacetate. Acetyl-CoA + citrate = acetate + (3S)-citryl-CoA.
Subunit structure	Oligomer with a subunit composition of (alpha,beta, gamma)6 By similarity.
Subcellular location	Cytoplasm.
Sequence caution	The sequence AAB40815.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Lyase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	acetyl-CoA metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	citrate lyase complex Inferred from electronic annotation. Source: InterPro
Molecular function	citrate (pro-3S)-lyase activity Inferred from electronic annotation. Source: EC citrate CoA-transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Citrate lyase alpha chain		PRO_0000089752

Sequences

	Sequence		Length	Mass (Da)
	P75726 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: CA466F1620D8EB92	FASTA	510	55,173
	10 20 30 40 50 60 MTQKIEQSQR QERVAAWNRR AECDLAAFQN SPKQTYQAEK ARDRKLCANL EEAIRRSGLQ 70 80 90 100 110 120 DGMTVSFHHA FRGGDLTVNM VMDVIAKMGF KNLTLASSSL SDCHAPLVEH IRQGVVTRIY 130 140 150 160 170 180 TSGLRGPLAE EISRGLLAEP VQIHSHGGRV HLVQSGELNI DVAFLGVPSC DEFGNANGYT 190 200 210 220 230 240 GKACCGSLGY AIVDADNAKQ VVMLTEELLP YPHNPASIEQ DQVDLIVKVD RVGDAAKIGA 250 260 270 280 290 300 GATRMTTNPR ELLIARSAAD VIVNSGYFKE GFSMQTGTGG ASLAVTRFLE DKMRSRDIRA 310 320 330 340 350 360 DFALGGITAT MVDLHEKGLI RKLLDVQSFD SHAAQSLARN PNHIEISANQ YANWGSKGAS 370 380 390 400 410 420 VDRLDVVVLS ALEIDTQFNV NVLTGSDGVL RGASGGHCDT AIASALSIIV APLVRGRIPT 430 440 450 460 470 480 LVDNVLTCIT PGSSVDILVT DHGIAVNPAR PELAERLQEA GIKVVSIEWL RERARLLTGE 490 500 510 PQPIEFTDRV VAVVRYRDGS VIDVVHQVKE

References

[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U82598 Genomic DNA. Translation: AAB40815.1. Different initiation. U00096 Genomic DNA. Translation: AAC73716.1. AP009048 Genomic DNA. Translation: BAA35251.2.
PIR	E64795.
RefSeq	NP_415148.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P75726.
SMR	P75726. Positions 30-509.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002118; EBESCP00000002118; EBESCG00000001735. EBESCT00000017156; EBESCP00000016447; EBESCG00000016215.
GeneID	945230.
GenomeReviews	Gene locus JW5087 in contig AP009048_GR. Gene locus b0615 in contig U00096_GR.
KEGG	ecj:JW5087. eco:b0615.
PATRIC	32116410. VBIEscCol129921_0645.
Organism-specific databases
EchoBASE	EB3311.
EcoGene	EG13541. citF.
Phylogenomic databases
eggNOG	COG3051.
GeneTree	EBGT00050000009608.
HOGENOM	HBG297040.
OMA	KGSVINQ.
PhylomeDB	P75726.
ProtClustDB	CLSK874113.
Enzyme and pathway databases
BioCyc	EcoCyc:CITTRANS-MONOMER. MetaCyc:CITTRANS-MONOMER.
Gene expression databases
Genevestigator	P75726.
Family and domain databases
InterPro	IPR006472. Citrate_lyase_asu. [Graphical view]
KO	K01643.
Pfam	PF04223. CitF. 1 hit. [Graphical view]
PIRSF	PIRSF009451. Citrt_lyas_alpha. 1 hit.
TIGRFAMs	TIGR01584. CitF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CILA_ECOLI

Accession

Primary (citable) accession number: P75726
Secondary accession number(s): P77102, Q9R7T5, Q9R7T6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program