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Protein

(S)-ureidoglycine aminohydrolase

Gene

allE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the catabolism of purine nucleotides. Catalyzes the second, stereospecific hydrolysis reaction of the allantoin degradation pathway, producing S-ureidoglycolate and ammonia from S-ureidoglycine.2 Publications

Catalytic activityi

(S)-2-ureidoglycine + H2O = (S)-ureidoglycolate + NH3.1 Publication

Cofactori

Mn2+1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi196ManganeseBy similarity1
Binding sitei196SubstrateBy similarity1
Metal bindingi198ManganeseBy similarity1
Metal bindingi202ManganeseBy similarity1
Metal bindingi236ManganeseBy similarity1
Binding sitei236SubstrateBy similarity1
Binding sitei249SubstrateBy similarity1
Binding sitei253SubstrateBy similarity1

GO - Molecular functioni

  • manganese ion binding Source: EcoCyc
  • ureidoglycine aminohydrolase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Purine metabolism

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6284-MONOMER.
ECOL316407:JW0503-MONOMER.
MetaCyc:G6284-MONOMER.
BRENDAi3.5.3.26. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
(S)-ureidoglycine aminohydrolase (EC:3.5.3.26)
Short name:
UGHY
Short name:
UGlyAH
Gene namesi
Name:allE
Synonyms:glxB6, ylbA
Ordered Locus Names:b0515, JW0503
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13622. ylbA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001686441 – 261(S)-ureidoglycine aminohydrolaseAdd BLAST261

Proteomic databases

PaxDbiP75713.
PRIDEiP75713.

Expressioni

Inductioni

By glyoxylate and allantoin under anaerobic conditions.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262016. 7 interactors.
DIPiDIP-12689N.
IntActiP75713. 7 interactors.
STRINGi511145.b0515.

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi11 – 14Combined sources4
Beta strandi25 – 29Combined sources5
Helixi31 – 33Combined sources3
Beta strandi42 – 49Combined sources8
Helixi53 – 55Combined sources3
Beta strandi58 – 74Combined sources17
Beta strandi81 – 96Combined sources16
Beta strandi99 – 104Combined sources6
Beta strandi107 – 111Combined sources5
Beta strandi118 – 121Combined sources4
Beta strandi123 – 125Combined sources3
Beta strandi127 – 135Combined sources9
Beta strandi147 – 150Combined sources4
Helixi151 – 153Combined sources3
Beta strandi166 – 169Combined sources4
Beta strandi178 – 186Combined sources9
Beta strandi194 – 200Combined sources7
Beta strandi202 – 217Combined sources16
Beta strandi219 – 223Combined sources5
Beta strandi227 – 230Combined sources4
Beta strandi232 – 240Combined sources9
Beta strandi247 – 254Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RC6X-ray2.60A/B1-261[»]
ProteinModelPortaliP75713.
SMRiP75713.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP75713.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi98 – 101Poly-Ser4
Compositional biasi204 – 207Poly-Leu4

Sequence similaritiesi

Belongs to the UGHY family.Curated

Phylogenomic databases

eggNOGiENOG4105S0E. Bacteria.
COG3257. LUCA.
HOGENOMiHOG000219622.
InParanoidiP75713.
KOiK14977.
OMAiLNRDWVE.
PhylomeDBiP75713.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR017627. CHP03214_cupin.
IPR013096. Cupin_2.
IPR008579. DUF861_Cupin_3.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF07883. Cupin_2. 1 hit.
PF05899. Cupin_3. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR03214. ura-cupin. 1 hit.

Sequencei

Sequence statusi: Complete.

P75713-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGYLNNVTGY REDLLANRAI VKHGNFALLT PDGLVKNIIP GFENCDATIL
60 70 80 90 100
STPKLGASFV DYLVTLHQNG GNQQGFGGEG IETFLYVISG NITAKAEGKT
110 120 130 140 150
FALSEGGYLY CPPGSLMTFV NAQAEDSQIF LYKRRYVPVE GYAPWLVSGN
160 170 180 190 200
ASELERIHYE GMDDVILLDF LPKELGFDMN MHILSFAPGA SHGYIETHVQ
210 220 230 240 250
EHGAYILSGQ GVYNLDNNWI PVKKGDYIFM GAYSLQAGYG VGRGEAFSYI
260
YSKDCNRDVE I
Length:261
Mass (Da):28,730
Last modified:February 1, 1997 - v1
Checksum:iB424C7DBD34508D6
GO

Sequence cautioni

The sequence AAB93856 differs from that shown. Reason: Frameshift at position 222.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti204A → T in AAB93856 (PubMed:10601204).Curated1
Sequence conflicti204A → T in AAB40267 (Ref. 2) Curated1
Sequence conflicti225 – 261GDYIF…RDVEI → RRLHLYGRLFFTGWLWCRAW in AAB40267 (Ref. 2) CuratedAdd BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89279 Genomic DNA. Translation: AAB93856.1. Frameshift.
U82664 Genomic DNA. Translation: AAB40267.1.
U00096 Genomic DNA. Translation: AAC73617.1.
AP009048 Genomic DNA. Translation: BAE76293.1.
PIRiB64783.
RefSeqiNP_415048.1. NC_000913.3.
WP_000540997.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73617; AAC73617; b0515.
BAE76293; BAE76293; BAE76293.
GeneIDi945149.
KEGGiecj:JW0503.
eco:b0515.
PATRICi32116187. VBIEscCol129921_0535.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89279 Genomic DNA. Translation: AAB93856.1. Frameshift.
U82664 Genomic DNA. Translation: AAB40267.1.
U00096 Genomic DNA. Translation: AAC73617.1.
AP009048 Genomic DNA. Translation: BAE76293.1.
PIRiB64783.
RefSeqiNP_415048.1. NC_000913.3.
WP_000540997.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RC6X-ray2.60A/B1-261[»]
ProteinModelPortaliP75713.
SMRiP75713.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262016. 7 interactors.
DIPiDIP-12689N.
IntActiP75713. 7 interactors.
STRINGi511145.b0515.

Proteomic databases

PaxDbiP75713.
PRIDEiP75713.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73617; AAC73617; b0515.
BAE76293; BAE76293; BAE76293.
GeneIDi945149.
KEGGiecj:JW0503.
eco:b0515.
PATRICi32116187. VBIEscCol129921_0535.

Organism-specific databases

EchoBASEiEB3387.
EcoGeneiEG13622. ylbA.

Phylogenomic databases

eggNOGiENOG4105S0E. Bacteria.
COG3257. LUCA.
HOGENOMiHOG000219622.
InParanoidiP75713.
KOiK14977.
OMAiLNRDWVE.
PhylomeDBiP75713.

Enzyme and pathway databases

BioCyciEcoCyc:G6284-MONOMER.
ECOL316407:JW0503-MONOMER.
MetaCyc:G6284-MONOMER.
BRENDAi3.5.3.26. 2026.

Miscellaneous databases

EvolutionaryTraceiP75713.
PROiP75713.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR017627. CHP03214_cupin.
IPR013096. Cupin_2.
IPR008579. DUF861_Cupin_3.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF07883. Cupin_2. 1 hit.
PF05899. Cupin_3. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR03214. ura-cupin. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiALLE_ECOLI
AccessioniPrimary (citable) accession number: P75713
Secondary accession number(s): O54410, P77128, Q2MBR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.