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P75611 (TKT_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transketolase
Short name=TK
EC=2.2.1.1
Gene names
Name:tkt
Synonyms:tktA
Ordered Locus Names:MPN_082
ORF Names:MP073
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma ›

Protein attributes

Sequence length648 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate By similarity.

Catalytic activity

Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate.

Cofactor

Binds 1 magnesium ion per subunit. Can also utilize other divalent metal cations, such as Ca2+, Mn2+ and Co2+ By similarity.

Binds 1 thiamine pyrophosphate per subunit By similarity.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the transketolase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 648648Transketolase
PRO_0000191862

Regions

Nucleotide binding109 – 1113Thiamine pyrophosphate By similarity

Sites

Active site3971Proton donor By similarity
Metal binding1501Magnesium By similarity
Metal binding1801Magnesium By similarity
Metal binding1821Magnesium; via carbonyl oxygen By similarity
Binding site221Substrate By similarity
Binding site621Thiamine pyrophosphate By similarity
Binding site1511Thiamine pyrophosphate; via amide nitrogen By similarity
Binding site1801Thiamine pyrophosphate By similarity
Binding site2521Substrate By similarity
Binding site2521Thiamine pyrophosphate By similarity
Binding site3451Substrate By similarity
Binding site3721Substrate By similarity
Binding site4231Thiamine pyrophosphate By similarity
Binding site4471Substrate By similarity
Binding site4551Substrate By similarity
Binding site5061Substrate By similarity
Site221Important for catalytic activity By similarity
Site2521Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
P75611 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: EFE8247D7F0837C9

FASTA64872,378
        10         20         30         40         50         60 
MKNLFACQHL ALSAIQHAKG GHVGMALGAS PILYTLWTKH IQFNPNCPKW INRDRLVMSA 

        70         80         90        100        110        120 
GHGSMALYPI LHFAGLITKQ EMLHHKYGQV NTSSHPEYAP NNFIDASTGP LGQGLGMAVG 

       130        140        150        160        170        180 
MALTQRVLAA EFKALSPKLF DHFTYVVVGD GDLQEGVSYE VAHLAGVYQL NKLIVLHDSN 

       190        200        210        220        230        240 
RVQMDSVVRD VSLENLQTRF TNMGWNYLET SDAVADIDAA IKQAKKSDKP TFIEVHTTIA 

       250        260        270        280        290        300 
KNTTLEDQPA GHWFIPTDKD FARFNSNTKT NFTPFEYPQT VYDFFHKQVI ARQAKPVQAY 

       310        320        330        340        350        360 
KELLEKLKDK PLYTKFINWT ENDYQALYLN QLDERKVAQA NAATRNYLKD FLGQINNSNS 

       370        380        390        400        410        420 
NLYCLNADVA RSCNIKLGDD NLHTNPHSRN IQVGIREFGM STIMNGMALH GGVKVMGGTF 

       430        440        450        460        470        480 
LAFADYSKPA IRLGALMNLP TFYVYTHDSY QVGGDGPTHQ PYDQLPMLRA IENVQVWRPC 

       490        500        510        520        530        540 
DEKETAAGVN YGLLSQDQTN VLILTRQALP SLEQSDSVQT LKGGYIISNR KQPDVIVAAS 

       550        560        570        580        590        600 
GSEVQLALQL EQALNEQQLK TRVVSVPNIN MLLSQPQSYL QQLFDPNSVL LTLEASASME 

       610        620        630        640 
WYALAKYVKK HTHLGAFSFG ESNDGQVVYE HKGFNVTNLL KLIKTLKS

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.
[2]"Towards a two-dimensional proteome map of Mycoplasma pneumoniae."
Regula J.T., Ueberle B., Boguth G., Goerg A., Schnoelzer M., Herrmann R., Frank R.
Electrophoresis 21:3765-3780(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U00089 Genomic DNA. Translation: AAB95721.1.
PIRS73399.
RefSeqNP_109770.1. NC_000912.1.

3D structure databases

ProteinModelPortalP75611.
ModBaseSearch...

Protein-protein interaction databases

STRING272634.MPN082.

Proteomic databases

PaxDbP75611.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB95721; AAB95721; MPN_082.
GeneID877050.
KEGGmpn:MPN082.
PATRIC20021447. VBIMycPne110_0084.

Phylogenomic databases

eggNOGCOG0021.
KOK00615.
OMAQPWYKYV.
ProtClustDBPRK05899.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-583.
MPNE272634:GJ6Z-85-MONOMER.

Family and domain databases

Gene3D3.40.50.920. 1 hit.
InterProIPR009014. Transketo_C/Pyr-ferredox_oxred.
IPR015941. Transketolase-like_C.
IPR005475. Transketolase-like_Pyr-bd.
IPR005478. Transketolase_bac-like.
IPR020826. Transketolase_BS.
IPR005474. Transketolase_N.
[Graphical view]
PfamPF02779. Transket_pyr. 1 hit.
PF00456. Transketolase_N. 1 hit.
[Graphical view]
SMARTSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMSSF52922. Transketo_C_like. 1 hit.
TIGRFAMsTIGR00232. tktlase_bact. 1 hit.
PROSITEPS00801. TRANSKETOLASE_1. False negative.
PS00802. TRANSKETOLASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTKT_MYCPN
AccessionPrimary (citable) accession number: P75611
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names

SIMILARITY comments

Index of protein domains and families

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