P75587 (RIBF_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative riboflavin biosynthesis protein RibF

Including the following 2 domains:

1. Riboflavin kinase
   EC=2.7.1.26
   Alternative name(s):
   Flavokinase
2. FMN adenylyltransferase
   EC=2.7.7.2
   Alternative name(s):
   FAD pyrophosphorylase
   FAD synthase

Gene names

Name:	ribF
Ordered Locus Names:	MPN_158
ORF Names:	MP673

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma pneumoniae

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + riboflavin = ADP + FMN. ATP + FMN = diphosphate + FAD.
Pathway	Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1. Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.
Sequence similarities	Belongs to the RibF family.

Ontologies

Keywords
Ligand	ATP-binding FAD FMN Nucleotide-binding
Molecular function	Kinase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	FAD biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway FMN biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW FMN adenylyltransferase activity Inferred from electronic annotation. Source: EC riboflavin kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-2260090,EBI-2260090

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Putative riboflavin biosynthesis protein RibF		PRO_0000194146

Sequences

	Sequence		Length	Mass (Da)
	P75587 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 2E63D7BC7A8FA12D	FASTA	269	30,435
	10 20 30 40 50 60 MQQTLIIGAF DGLHKGHQLL AQAASGPVVA LLIANIPSLQ SDWLYEPKQR QVQLQQHFQS 70 80 90 100 110 120 VVHSYDVIEH NISAQAFFDQ IISPLRCQQL VVGADFCFGK DNQNADFLRR LFPNTTIIPK 130 140 150 160 170 180 DSQTLSSSTI RQWLKQGQIE QANAVLLEPY FREGVVIRGN QQARFLGWPT ANITLKPYMV 190 200 210 220 230 240 PLRCGSYVIT VTYHQTNYPG VGFISYKNDQ LVCETHLIGF SGDLYGKQLR FTFNQFIRPQ 250 260 QKFSGVQALQ KAISGDLKQA QKWFAQSTN

References

[1]

"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB96321.1.
PIR	S73999.
RefSeq	NP_109846.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P75587.
ModBase	Search...
Protein-protein interaction databases
IntAct	P75587. 1 interaction.
STRING	272634.MPN158.
Proteomic databases
PaxDb	P75587.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB96321; AAB96321; MPN_158.
GeneID	877209.
KEGG	mpn:MPN158.
PATRIC	20021639. VBIMycPne110_0176.
Phylogenomic databases
eggNOG	COG0196.
KO	K11753.
OMA	CETHIFN.
ProtClustDB	CLSK335159.
Enzyme and pathway databases
BioCyc	MPNE272634:GJ6Z-165-MONOMER.
UniPathway	UPA00276; UER00406. UPA00277; UER00407.
Family and domain databases
Gene3D	2.40.30.30. 1 hit. 3.40.50.620. 1 hit.
InterPro	IPR015864. FAD_synthase. IPR023468. Riboflavin_kinase. IPR002606. Riboflavin_kinase_bac. IPR015865. Riboflavin_kinase_bac/euk. IPR023465. Riboflavin_kinase_domain. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR22749. PTHR22749. 1 hit.
Pfam	PF06574. FAD_syn. 1 hit. PF01687. Flavokinase. 1 hit. [Graphical view]
PIRSF	PIRSF004491. FAD_Synth. 1 hit.
SMART	SM00904. Flavokinase. 1 hit. [Graphical view]
SUPFAM	SSF82114. Riboflavin_kinase. 1 hit.
TIGRFAMs	TIGR00083. ribF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBF_MYCPN

Accession

Primary (citable) accession number: P75587

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program