P75569 (PTG3C_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
PTS system glucose-specific EIICBA component

Alternative name(s):
EII-Glc/EIII-Glc
EIICBA-Glc

Including the following 3 domains:

1. Glucose permease IIC component
   Alternative name(s):
   PTS system glucose-specific EIIC component
2. Glucose-specific phosphotransferase enzyme IIB component
   EC=2.7.1.69
   Alternative name(s):
   PTS system glucose-specific EIIB component
3. Glucose-specific phosphotransferase enzyme IIA component
   EC=2.7.1.-
   Alternative name(s):
   PTS system glucose-specific EIIA component

Gene names

Name:	ptsG
Ordered Locus Names:	MPN_207
ORF Names:	MP624

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129)

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	940 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport By similarity.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine. Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Sequence similarities	Contains 1 PTS EIIA type-1 domain. Contains 1 PTS EIIB type-1 domain. Contains 1 PTS EIIC type-1 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 940	940	PTS system glucose-specific EIICBA component		PRO_0000186560
Regions
	Transmembrane	43 – 63	21	Helical; Potential
	Transmembrane	83 – 103	21	Helical; Potential
	Transmembrane	112 – 132	21	Helical; Potential
	Transmembrane	175 – 195	21	Helical; Potential
	Transmembrane	209 – 229	21	Helical; Potential
	Transmembrane	487 – 507	21	Helical; Potential
	Transmembrane	515 – 535	21	Helical; Potential
	Transmembrane	537 – 557	21	Helical; Potential
	Transmembrane	564 – 584	21	Helical; Potential
	Transmembrane	598 – 618	21	Helical; Potential
	Domain	1 – 284	284	PTS EIIC type-1; first part
	Domain	479 – 630	152	PTS EIIC type-1; second part
	Domain	661 – 743	83	PTS EIIB type-1
	Domain	794 – 907	114	PTS EIIA type-1
	Region	285 – 478	194	Unknown
Sites
	Active site	683	1	Phosphocysteine intermediate; for EIIB activity By similarity
	Active site	847	1	Tele-phosphohistidine intermediate; for EIIA activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P75569 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 44B836307FDA36EF	FASTA	940	101,619
	10 20 30 40 50 60 MQIKAQDTGQ QKKSCLLSNI RNKWKNRNRG SFRQWVGKLS NGLMIPIAVL PIAGIFLGVG 70 80 90 100 110 120 DAIAGNAGDL TGLRYFGLFI KNGGDVVFAN LPILFAIAIA ITFSQDAGVA GFSAFVFWAA 130 140 150 160 170 180 MNGFMSSLIL PFDKAGKIIT DTSTPIAGFK VLYNKSVPVH AIATTLGLRT LSTSVFGGII 190 200 210 220 230 240 VGALTSVLYK KFYAIRLPDV IGFFSGTRFV PIICFVVAIP VALILLMIWP AVSIGLNAIG 250 260 270 280 290 300 TGLGFLGGKG YGANSLIFGY IERSLIPFGV HHAFYAPLWY TSAGGSLQEI VNQQVWIRPD 310 320 330 340 350 360 FHLSDNYVAR VIGWVDPNNS SMYIIPGALN GQNGSSTGNT MSKDLNGALS AYMSKESTAF 370 380 390 400 410 420 LTWKDLVDGL TFKGNFDKMA ENGLLDGSNK IWLGLNGSGI LGKKLLLSDG NVYTITFKTF 430 440 450 460 470 480 ANTTPIAWSK GAQAVLPLNA SSTIVNNPTA LAAATQSNNN TNNIKLYPVN SFRVAVESLN 490 500 510 520 530 540 PAQYSQGKFP FMLFGIPAAG VAMILAAPKD RRKEAASIVG SAAFTSFLTG ITEPFEFTFL 550 560 570 580 590 600 FLAPWLFYGV HAVLAAVSFW LMNILGANVG QTFSGSFIDF ILYGALPDGR RWLANSYLVP 610 620 630 640 650 660 IIGLFLAAIY FPTFYFLIKH FNLATPGRGG KLITKKEYLA SKAAAKAEGV SGVAENFTQT 670 680 690 700 710 720 QIEAGILLQA YGGKENIVEL GACITKLRVT VKNPELVKEE PIKELGAAGV MRTTPTFFVA 730 740 750 760 770 780 VFGTRAAVYK SAMQDIIQGK VNWEALQKVI NTDQLAVEPK ETTPPKEVMP VVQDEIVILS 790 800 810 820 830 840 PVNGTLKSLN QVPDETFKQK LVGEGVAIVP SDGHFKAPGE AGVKTELAFP GGHAYIFDID 850 860 870 880 890 900 GIKVMLHIGI DTVQINAKKQ PGEPLEVFDI KTKQGEYTKE KSESVVEVDL KKLSKKYNPI 910 920 930 940 TPFVVMKESL ENFKLVPIRQ RGEIKVGQPI FKLVYKKSQA

References

[1]	"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB96272.1.
PIR	S73950.
RefSeq	NP_109895.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P75569.
ModBase	Search...
Protein-protein interaction databases
IntAct	P75569. 4 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	876972.
GenomeReviews	Gene locus MPN_207 in contig U00089_GR.
KEGG	mpn:MPN207.
PATRIC	20021739. VBIMycPne110_0226.
Phylogenomic databases
HOGENOM	HBG571563.
OMA	IFGYIER.
PhylomeDB	P75569.
ProtClustDB	CLSK542132.
Enzyme and pathway databases
BioCyc	MPNE272634:MPN207-MONOMER.
Family and domain databases
InterPro	IPR011055. Dup_hybrid_motif. IPR018113. PTrfase_EIIB/Cys_phosph_CS. IPR001127. PTS_EIIA_1_perm. IPR001996. PTS_EIIB_1. IPR003352. PTS_EIIC. IPR013013. PTS_EIIC_1. IPR011535. PTS_Glc-like_IIB_component. [Graphical view]
Gene3D	G3DSA:3.30.1360.60. PTS_EIIB. 1 hit.
KO	K02777. K02778. K02779.
Pfam	PF00358. PTS_EIIA_1. 1 hit. PF00367. PTS_EIIB. 1 hit. PF02378. PTS_EIIC. 2 hits. [Graphical view]
SUPFAM	SSF51261. Dup_hybrid_motif. 1 hit. SSF55604. PTS_EIIB. 1 hit.
TIGRFAMs	TIGR00826. EIIB_glc. 1 hit. TIGR00830. PTBA. 1 hit.
PROSITE	PS51093. PTS_EIIA_TYPE_1. 1 hit. PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit. PS51098. PTS_EIIB_TYPE_1. 1 hit. PS51103. PTS_EIIC_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTG3C_MYCPN

Accession

Primary (citable) accession number: P75569

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program