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P75534 (GATA_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A

Short name=Glu-ADT subunit A
EC=6.3.5.7
Gene names
Name:gatA
Ordered Locus Names:MPN_237
ORF Names:MP594
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00120

Catalytic activity

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00120

Subunit structure

Heterotrimer of A, B and C subunits By similarity. HAMAP-Rule MF_00120

Sequence similarities

Belongs to the amidase family. GatA subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtranslation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 478478Glutamyl-tRNA(Gln) amidotransferase subunit A HAMAP-Rule MF_00120
PRO_0000105179

Sites

Active site681Charge relay system By similarity
Active site1431Charge relay system By similarity
Active site1671Acyl-ester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
P75534 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: F228E9AE4F641071

FASTA47853,230
        10         20         30         40         50         60 
MKSQILKLQQ TLTKKPASIN PLLQQIDGAI NEHWSSNFLL KNTVEWAQAQ APKNRSKSPL 

        70         80         90        100        110        120 
NNIPFVLKDN IATKGIVTTG GSRFLEDYIP PFSATVFELL NNSGALLVGK ANLDEFGLGG 

       130        140        150        160        170        180 
TGLHSGFGFV HHPWNETLIP GGSSSGSAYA VARGIVPFSI GTDTGDSVRR PASICNIVGF 

       190        200        210        220        230        240 
KPTYGLISRN GVYPYAPSLD HVGIFARYVY DVALVSDEII KHDKADFSAQ KSPDAGKFTR 

       250        260        270        280        290        300 
SLKESFNKQI KIGYLKPLEE WFDIELSKKW NSLKERITLE GCELIPFHFP LELLEVIDPV 

       310        320        330        340        350        360 
YKLISYSEAV SCYSNLTGIV FGQKLFEPNQ ASDFSKTITA NRDRFFGEQL KRRFIIGAFG 

       370        380        390        400        410        420 
TDKNNFTKYF EKAQKIRRVM VDAYLNLFKE ADFIVSPSAS GFTKTIAAVQ KGESFTNLVD 

       430        440        450        460        470 
DFLQLANFAG NPSITIPWLV KQKDQTIGLS VNANCFHDKQ LLQVAAWLEE LFQIEHDD 

« Hide

References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB96242.1.
PIRS73920.
RefSeqNP_109925.1. NC_000912.1.

3D structure databases

ProteinModelPortalP75534.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272634.MPN237.

Proteomic databases

PaxDbP75534.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB96242; AAB96242; MPN_237.
GeneID877201.
KEGGmpn:MPN237.
PATRIC20021799. VBIMycPne110_0256.

Phylogenomic databases

eggNOGCOG0154.
KOK02433.
OMAYDILLAY.
OrthoDBEOG61P6R9.

Enzyme and pathway databases

BioCycMPNE272634:GJ6Z-244-MONOMER.

Family and domain databases

Gene3D3.90.1300.10. 1 hit.
HAMAPMF_00120. GatA.
InterProIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERPTHR11895. PTHR11895. 1 hit.
PfamPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMSSF75304. SSF75304. 1 hit.
TIGRFAMsTIGR00132. gatA. 1 hit.
PROSITEPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGATA_MYCPN
AccessionPrimary (citable) accession number: P75534
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 14, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names