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P75527

- DEF_MYCPN

UniProt

P75527 - DEF_MYCPN

Protein

Peptide deformylase

Gene

def

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity.By similarity

    Catalytic activityi

    Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.

    Cofactori

    Binds 1 Fe2+ ion.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi134 – 1341IronBy similarity
    Metal bindingi178 – 1781IronBy similarity
    Active sitei179 – 1791By similarity
    Metal bindingi182 – 1821IronBy similarity

    GO - Molecular functioni

    1. iron ion binding Source: InterPro
    2. peptide deformylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. translation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciMPNE272634:GJ6Z-252-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peptide deformylase (EC:3.5.1.88)
    Short name:
    PDF
    Alternative name(s):
    Polypeptide deformylase
    Gene namesi
    Name:def
    Ordered Locus Names:MPN_245
    ORF Names:MP587
    OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
    Taxonomic identifieri272634 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000808: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 216216Peptide deformylasePRO_0000082803Add
    BLAST

    Proteomic databases

    PaxDbiP75527.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself1EBI-2259869,EBI-2259869

    Protein-protein interaction databases

    IntActiP75527. 1 interaction.
    STRINGi272634.MPN245.

    Structurei

    3D structure databases

    ProteinModelPortaliP75527.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the polypeptide deformylase family.Curated

    Phylogenomic databases

    eggNOGiCOG0242.
    KOiK01462.
    OMAiTISIFRI.
    OrthoDBiEOG6PZXGQ.

    Family and domain databases

    Gene3Di3.90.45.10. 1 hit.
    HAMAPiMF_00163. Pep_deformylase.
    InterProiIPR000181. Fmet_deformylase.
    IPR023635. Peptide_deformylase.
    [Graphical view]
    PANTHERiPTHR10458. PTHR10458. 1 hit.
    PfamiPF01327. Pep_deformylase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004749. Pep_def. 1 hit.
    PRINTSiPR01576. PDEFORMYLASE.
    SUPFAMiSSF56420. SSF56420. 1 hit.
    TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P75527-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKILPVSTI SIFRIILILP QINMELLPTK AWLVLDDVKE INEPTKPVQF    50
    PLDQASLDCI AKMMAYVDAS YNGDAEKYGI IPGIGIAANQ IGYWKQMFYI 100
    HLMDGGVEHK CLLINPKIIN LSANKSFLKS GEGCLSVPKM HQGYVIRHEW 150
    ITITGFDWLQ QKEITITATG LFGMCLQHEF DHLQGRFYYH RINPLNPLFT 200
    NKEWKVINPA LPSDSE 216
    Length:216
    Mass (Da):24,592
    Last modified:February 1, 1997 - v1
    Checksum:i361F434048B505DF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB96235.1.
    PIRiS73913.
    RefSeqiNP_109933.1. NC_000912.1.

    Genome annotation databases

    EnsemblBacteriaiAAB96235; AAB96235; MPN_245.
    GeneIDi876781.
    KEGGimpn:MPN245.
    PATRICi20021815. VBIMycPne110_0264.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB96235.1 .
    PIRi S73913.
    RefSeqi NP_109933.1. NC_000912.1.

    3D structure databases

    ProteinModelPortali P75527.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P75527. 1 interaction.
    STRINGi 272634.MPN245.

    Proteomic databases

    PaxDbi P75527.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB96235 ; AAB96235 ; MPN_245 .
    GeneIDi 876781.
    KEGGi mpn:MPN245.
    PATRICi 20021815. VBIMycPne110_0264.

    Phylogenomic databases

    eggNOGi COG0242.
    KOi K01462.
    OMAi TISIFRI.
    OrthoDBi EOG6PZXGQ.

    Enzyme and pathway databases

    BioCyci MPNE272634:GJ6Z-252-MONOMER.

    Family and domain databases

    Gene3Di 3.90.45.10. 1 hit.
    HAMAPi MF_00163. Pep_deformylase.
    InterProi IPR000181. Fmet_deformylase.
    IPR023635. Peptide_deformylase.
    [Graphical view ]
    PANTHERi PTHR10458. PTHR10458. 1 hit.
    Pfami PF01327. Pep_deformylase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004749. Pep_def. 1 hit.
    PRINTSi PR01576. PDEFORMYLASE.
    SUPFAMi SSF56420. SSF56420. 1 hit.
    TIGRFAMsi TIGR00079. pept_deformyl. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
      Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
      Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29342 / M129.

    Entry informationi

    Entry nameiDEF_MYCPN
    AccessioniPrimary (citable) accession number: P75527
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Mycoplasma pneumoniae
      Mycoplasma pneumoniae (strain M129): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3