Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P75527 (DEF_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptide deformylase

Short name=PDF
EC=3.5.1.88
Alternative name(s):
Polypeptide deformylase
Gene names
Name:def
Ordered Locus Names:MPN_245
ORF Names:MP587
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length216 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP-Rule MF_00163

Catalytic activity

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP-Rule MF_00163

Cofactor

Binds 1 Fe2+ ion By similarity. HAMAP-Rule MF_00163

Sequence similarities

Belongs to the polypeptide deformylase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandIron
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtranslation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functioniron ion binding

Inferred from electronic annotation. Source: InterPro

peptide deformylase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself1EBI-2259869,EBI-2259869

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 216216Peptide deformylase HAMAP-Rule MF_00163
PRO_0000082803

Sites

Active site1791 By similarity
Metal binding1341Iron By similarity
Metal binding1781Iron By similarity
Metal binding1821Iron By similarity

Sequences

Sequence LengthMass (Da)Tools
P75527 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 361F434048B505DF

FASTA21624,592
        10         20         30         40         50         60 
MTKILPVSTI SIFRIILILP QINMELLPTK AWLVLDDVKE INEPTKPVQF PLDQASLDCI 

        70         80         90        100        110        120 
AKMMAYVDAS YNGDAEKYGI IPGIGIAANQ IGYWKQMFYI HLMDGGVEHK CLLINPKIIN 

       130        140        150        160        170        180 
LSANKSFLKS GEGCLSVPKM HQGYVIRHEW ITITGFDWLQ QKEITITATG LFGMCLQHEF 

       190        200        210 
DHLQGRFYYH RINPLNPLFT NKEWKVINPA LPSDSE 

« Hide

References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB96235.1.
PIRS73913.
RefSeqNP_109933.1. NC_000912.1.

3D structure databases

ProteinModelPortalP75527.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP75527. 1 interaction.
STRING272634.MPN245.

Proteomic databases

PaxDbP75527.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB96235; AAB96235; MPN_245.
GeneID876781.
KEGGmpn:MPN245.
PATRIC20021815. VBIMycPne110_0264.

Phylogenomic databases

eggNOGCOG0242.
KOK01462.
OMATISIFRI.
OrthoDBEOG6PZXGQ.

Enzyme and pathway databases

BioCycMPNE272634:GJ6Z-252-MONOMER.

Family and domain databases

Gene3D3.90.45.10. 1 hit.
HAMAPMF_00163. Pep_deformylase.
InterProIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
PANTHERPTHR10458. PTHR10458. 1 hit.
PfamPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFPIRSF004749. Pep_def. 1 hit.
PRINTSPR01576. PDEFORMYLASE.
SUPFAMSSF56420. SSF56420. 1 hit.
TIGRFAMsTIGR00079. pept_deformyl. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDEF_MYCPN
AccessionPrimary (citable) accession number: P75527
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 14, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names