P75500 (SYK_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysine--tRNA ligase
EC=6.1.1.6

Alternative name(s):
Lysyl-tRNA synthetase
Short name=LysRS

Gene names

Name:	lysS
Ordered Locus Names:	MPN_277
ORF Names:	MP558

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma pneumoniae

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP-Rule MF_00252
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP lysine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 489	489	Lysine--tRNA ligase HAMAP-Rule MF_00252		PRO_0000152651
Sites
	Metal binding	399	1	Magnesium 1 By similarity
	Metal binding	406	1	Magnesium 1 By similarity
	Metal binding	406	1	Magnesium 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P75500 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: E3D643B73DEDBCA0	FASTA	489	56,081
	10 20 30 40 50 60 MSDRLNDQAQ NRLQKLLNLK QTGNDPYLIT KIENTHSAAS FQKAFANQSD AELKQYQVIL 70 80 90 100 110 120 TGRIIALRQT FIIIQDFSGK MQLYINKKTA PELFEYFNNY IDLGDQIVAT GHPMMTKTGV 130 140 150 160 170 180 LTLNVERLQI VAKCLQTPPE KWHGLTDPEA RARKRYLDLT YNRAQVEVFL KRTQIITAIR 190 200 210 220 230 240 TFLNEAGFLE VETPILQAVL GGANAKPFKT HYNALKGDFY LRIANEIALK KLIVGGLPKV 250 260 270 280 290 300 YEMGRMFRNE GVDTTHNPEF TSIEMYQANA DYAVMMDLTE NLIKFVCKTL NQWSFNWNGQ 310 320 330 340 350 360 TLDLAKPFKK VKMVDLICQV TGVNFDDVKD DQTAIALAKQ HKVELKKHEQ NKQHIINRFF 370 380 390 400 410 420 EQFCEHTLIQ PTFVTHYPKA VSPLAKQDPH NPEFTERFEL FINTKELANA YSELNDPLEQ 430 440 450 460 470 480 RARFEQQLQE KRMGNDEASE LDESFLDALS FGLPPTGGLG IGVDRLVMLL CECSSIRDVV FFPQLRELK

References

[1]

"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB96206.1.
PIR	S73884.
RefSeq	NP_109965.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P75500.
ModBase	Search...
Protein-protein interaction databases
IntAct	P75500. 2 interactions.
STRING	272634.MPN277.
Proteomic databases
PaxDb	P75500.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB96206; AAB96206; MPN_277.
GeneID	876870.
KEGG	mpn:MPN277.
PATRIC	20021881. VBIMycPne110_0297.
Phylogenomic databases
eggNOG	COG1190.
KO	K04567.
OMA	VTFHNAL.
ProtClustDB	PRK00484.
Enzyme and pathway databases
BioCyc	MPNE272634:GJ6Z-284-MONOMER.
Family and domain databases
Gene3D	2.40.50.140. 1 hit.
HAMAP	MF_00252. Lys_tRNA_synth_class2.
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR002313. Lys-tRNA-ligase_II. IPR018149. Lys-tRNA-synth_II_C. IPR012340. NA-bd_OB-fold. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
PANTHER	PTHR22594. PTHR22594. 1 hit. PTHR22594:SF4. PTHR22594:SF4. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR00982. TRNASYNTHLYS.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00499. lysS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYK_MYCPN

Accession

Primary (citable) accession number: P75500

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program