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P75500 (SYK_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lysine--tRNA ligase

EC=6.1.1.6
Alternative name(s):
Lysyl-tRNA synthetase
Short name=LysRS
Gene names
Name:lysS
Ordered Locus Names:MPN_277
ORF Names:MP558
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length489 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP-Rule MF_00252

Cofactor

Binds 3 magnesium ions per subunit By similarity. HAMAP-Rule MF_00252

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00252

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00252.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processlysyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

lysine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 489489Lysine--tRNA ligase HAMAP-Rule MF_00252
PRO_0000152651

Sites

Metal binding3991Magnesium 1 By similarity
Metal binding4061Magnesium 1 By similarity
Metal binding4061Magnesium 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
P75500 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: E3D643B73DEDBCA0

FASTA48956,081
        10         20         30         40         50         60 
MSDRLNDQAQ NRLQKLLNLK QTGNDPYLIT KIENTHSAAS FQKAFANQSD AELKQYQVIL 

        70         80         90        100        110        120 
TGRIIALRQT FIIIQDFSGK MQLYINKKTA PELFEYFNNY IDLGDQIVAT GHPMMTKTGV 

       130        140        150        160        170        180 
LTLNVERLQI VAKCLQTPPE KWHGLTDPEA RARKRYLDLT YNRAQVEVFL KRTQIITAIR 

       190        200        210        220        230        240 
TFLNEAGFLE VETPILQAVL GGANAKPFKT HYNALKGDFY LRIANEIALK KLIVGGLPKV 

       250        260        270        280        290        300 
YEMGRMFRNE GVDTTHNPEF TSIEMYQANA DYAVMMDLTE NLIKFVCKTL NQWSFNWNGQ 

       310        320        330        340        350        360 
TLDLAKPFKK VKMVDLICQV TGVNFDDVKD DQTAIALAKQ HKVELKKHEQ NKQHIINRFF 

       370        380        390        400        410        420 
EQFCEHTLIQ PTFVTHYPKA VSPLAKQDPH NPEFTERFEL FINTKELANA YSELNDPLEQ 

       430        440        450        460        470        480 
RARFEQQLQE KRMGNDEASE LDESFLDALS FGLPPTGGLG IGVDRLVMLL CECSSIRDVV 


FFPQLRELK 

« Hide

References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB96206.1.
PIRS73884.
RefSeqNP_109965.1. NC_000912.1.

3D structure databases

ProteinModelPortalP75500.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP75500. 2 interactions.
STRING272634.MPN277.

Proteomic databases

PaxDbP75500.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB96206; AAB96206; MPN_277.
GeneID876870.
KEGGmpn:MPN277.
PATRIC20021881. VBIMycPne110_0297.

Phylogenomic databases

eggNOGCOG1190.
KOK04567.
OMAFKAPYAR.
OrthoDBEOG69PQ2M.

Enzyme and pathway databases

BioCycMPNE272634:GJ6Z-284-MONOMER.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
HAMAPMF_00252. Lys_tRNA_synth_class2.
InterProIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR002313. Lys-tRNA-ligase_II.
IPR018149. Lys-tRNA-synth_II_C.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERPTHR22594. PTHR22594. 1 hit.
PfamPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSPR00982. TRNASYNTHLYS.
SUPFAMSSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00499. lysS_bact. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYK_MYCPN
AccessionPrimary (citable) accession number: P75500
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 11, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries