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P75498

- LEPA_MYCPN

UniProt

P75498 - LEPA_MYCPN

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Protein

Elongation factor 4

Gene

lepA

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 216GTPUniRule annotation
Nucleotide bindingi132 – 1354GTPUniRule annotation

GO - Molecular functioni

  1. GTPase activity Source: UniProtKB-HAMAP
  2. GTP binding Source: UniProtKB-HAMAP
  3. ribosome binding Source: UniProtKB-HAMAP
  4. translation elongation factor activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. positive regulation of translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMPNE272634:GJ6Z-286-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
Short name:
EF-4UniRule annotation
Alternative name(s):
Ribosomal back-translocase LepAUniRule annotation
Gene namesi
Name:lepAUniRule annotation
Ordered Locus Names:MPN_279
ORF Names:MP556
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000808: Chromosome

Subcellular locationi

Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 598598Elongation factor 4PRO_0000176305Add
BLAST

Proteomic databases

PaxDbiP75498.

Interactioni

Protein-protein interaction databases

STRINGi272634.MPN279.

Structurei

3D structure databases

ProteinModelPortaliP75498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 185182tr-type GAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0481.
KOiK03596.
OMAiCYTALEM.
OrthoDBiEOG6ZKXQ4.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA.
InterProiIPR006297. EF-4.
IPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P75498-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEQQKIRNFS IIAHIDHGKS TLSDRLIERS IGFEKRLLQA QMLDTMAIER
60 70 80 90 100
ERGITIKLNA VQLKMAQGNQ QYLFHLVDTP GHVDFTYEVS RSLAACEGVL
110 120 130 140 150
LLVDATQGIQ AQTISNTYLA LENNLEIIPV INKVDMESAD VEKTKQAFHQ
160 170 180 190 200
LLGVDPNTIP LVSAKTGLGI DQLITTIIEK VPPPKGDESK PLKALLFDSY
210 220 230 240 250
YDPYKGVVCF IRIFEGSLKL NDKIRFARSN SVYQIVELGI KNPFFEKQDV
260 270 280 290 300
LKAGEIGWFS AGIKKLRDVT VGDTIVHAED TTTPPLPGYK KVLPMIYCGL
310 320 330 340 350
YPIDNNDYQN LKMAMEKIIL SDVALEYEYE TSQALGFGVR CGFLGLLHMD
360 370 380 390 400
VIKERLEREY NLKLISAPPS VRYKVLLTNG EELELDNPSL LPERSRIKSI
410 420 430 440 450
SEPFVRVYID LPDHYLGTVI DLCQNFRGQY EKLEEIDIDR KRLVYLMPLG
460 470 480 490 500
EIIYSFFDKL KSITKGYASL NYEFDQYQVS QLAKVEIMLN KQKVDALSFI
510 520 530 540 550
AHHDFAFQRA KKFCVKLKEL IPKHLFEIPI QATIGSKVIA RETIKAVRKD
560 570 580 590
VTAKLYGGDV TRKKKLLEKQ KEGKKRLKAI GSVELPQELF SHLLKDED
Length:598
Mass (Da):68,025
Last modified:March 5, 2002 - v2
Checksum:i4E5A1A230763B57C
GO

Sequence cautioni

The sequence AAB96204.1 differs from that shown. Reason: Frameshift at position 540.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00089 Genomic DNA. Translation: AAB96204.1. Frameshift.
PIRiS73882.
RefSeqiNP_109967.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB96204; AAB96204; MPN_279.
GeneIDi876840.
KEGGimpn:MPN279.
PATRICi20021885. VBIMycPne110_0299.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00089 Genomic DNA. Translation: AAB96204.1 . Frameshift.
PIRi S73882.
RefSeqi NP_109967.1. NC_000912.1.

3D structure databases

ProteinModelPortali P75498.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272634.MPN279.

Proteomic databases

PaxDbi P75498.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB96204 ; AAB96204 ; MPN_279 .
GeneIDi 876840.
KEGGi mpn:MPN279.
PATRICi 20021885. VBIMycPne110_0299.

Phylogenomic databases

eggNOGi COG0481.
KOi K03596.
OMAi CYTALEM.
OrthoDBi EOG6ZKXQ4.

Enzyme and pathway databases

BioCyci MPNE272634:GJ6Z-286-MONOMER.

Family and domain databases

Gene3Di 3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPi MF_00071. LepA.
InterProi IPR006297. EF-4.
IPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view ]
Pfami PF00679. EFG_C. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view ]
PRINTSi PR00315. ELONGATNFCT.
SMARTi SM00838. EFG_C. 1 hit.
[Graphical view ]
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsi TIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEi PS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
    Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
    Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29342 / M129.
  2. Sinan C.
    Unpublished observations (FEB-2002)
    Cited for: IDENTIFICATION OF PROBABLE FRAMESHIFT.

Entry informationi

Entry nameiLEPA_MYCPN
AccessioniPrimary (citable) accession number: P75498
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 5, 2002
Last modified: October 29, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3