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Protein

Elongation factor 4

Gene

lepA

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.

Catalytic activityi

GTP + H2O = GDP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi16 – 21GTP6
Nucleotide bindingi132 – 135GTP4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 4 (EC:3.6.5.n1)
Short name:
EF-4
Alternative name(s):
Ribosomal back-translocase LepA
Gene namesi
Name:lepA
Ordered Locus Names:MPN_279
ORF Names:MP556
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000808 Componenti: Chromosome

Subcellular locationi

  • Cell membrane ; Peripheral membrane protein ; Cytoplasmic side

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001763051 – 598Elongation factor 4Add BLAST598

Structurei

3D structure databases

ProteinModelPortaliP75498.
SMRiP75498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 185tr-type GAdd BLAST182

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.

Phylogenomic databases

KOiK03596.
OMAiALIFDSW.

Family and domain databases

CDDicd03709. lepA_C. 1 hit.
HAMAPiMF_00071. LepA. 1 hit.
InterProiView protein in InterPro
IPR006297. EF-4.
IPR035647. EFG_III/V.
IPR000640. EFG_V-like.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR013842. LepA_CTD.
IPR035654. LepA_IV.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel_sf.
PfamiView protein in Pfam
PF00679. EFG_C. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P75498-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQQKIRNFS IIAHIDHGKS TLSDRLIERS IGFEKRLLQA QMLDTMAIER
60 70 80 90 100
ERGITIKLNA VQLKMAQGNQ QYLFHLVDTP GHVDFTYEVS RSLAACEGVL
110 120 130 140 150
LLVDATQGIQ AQTISNTYLA LENNLEIIPV INKVDMESAD VEKTKQAFHQ
160 170 180 190 200
LLGVDPNTIP LVSAKTGLGI DQLITTIIEK VPPPKGDESK PLKALLFDSY
210 220 230 240 250
YDPYKGVVCF IRIFEGSLKL NDKIRFARSN SVYQIVELGI KNPFFEKQDV
260 270 280 290 300
LKAGEIGWFS AGIKKLRDVT VGDTIVHAED TTTPPLPGYK KVLPMIYCGL
310 320 330 340 350
YPIDNNDYQN LKMAMEKIIL SDVALEYEYE TSQALGFGVR CGFLGLLHMD
360 370 380 390 400
VIKERLEREY NLKLISAPPS VRYKVLLTNG EELELDNPSL LPERSRIKSI
410 420 430 440 450
SEPFVRVYID LPDHYLGTVI DLCQNFRGQY EKLEEIDIDR KRLVYLMPLG
460 470 480 490 500
EIIYSFFDKL KSITKGYASL NYEFDQYQVS QLAKVEIMLN KQKVDALSFI
510 520 530 540 550
AHHDFAFQRA KKFCVKLKEL IPKHLFEIPI QATIGSKVIA RETIKAVRKD
560 570 580 590
VTAKLYGGDV TRKKKLLEKQ KEGKKRLKAI GSVELPQELF SHLLKDED
Length:598
Mass (Da):68,025
Last modified:March 5, 2002 - v2
Checksum:i4E5A1A230763B57C
GO

Sequence cautioni

The sequence AAB96204 differs from that shown. Reason: Frameshift at position 540.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB96204.1. Frameshift.
PIRiS73882.
RefSeqiNP_109967.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB96204; AAB96204; MPN_279.
GeneIDi876840.
KEGGimpn:MPN279.
PATRICifig|272634.6.peg.299.

Similar proteinsi

Entry informationi

Entry nameiLEPA_MYCPN
AccessioniPrimary (citable) accession number: P75498
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 5, 2002
Last modified: November 22, 2017
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families