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P75498

- LEPA_MYCPN

UniProt

P75498 - LEPA_MYCPN

Protein

Elongation factor 4

Gene

lepA

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (05 Mar 2002)
      Previous versions | rss
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    Functioni

    Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation

    Catalytic activityi

    GTP + H2O = GDP + phosphate.UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi16 – 216GTPUniRule annotation
    Nucleotide bindingi132 – 1354GTPUniRule annotation

    GO - Molecular functioni

    1. GTPase activity Source: UniProtKB-HAMAP
    2. GTP binding Source: UniProtKB-HAMAP
    3. ribosome binding Source: UniProtKB-HAMAP
    4. translation elongation factor activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. positive regulation of translation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMPNE272634:GJ6Z-286-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
    Short name:
    EF-4UniRule annotation
    Alternative name(s):
    Ribosomal back-translocase LepAUniRule annotation
    Gene namesi
    Name:lepAUniRule annotation
    Ordered Locus Names:MPN_279
    ORF Names:MP556
    OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
    Taxonomic identifieri272634 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000808: Chromosome

    Subcellular locationi

    Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 598598Elongation factor 4PRO_0000176305Add
    BLAST

    Proteomic databases

    PaxDbiP75498.

    Interactioni

    Protein-protein interaction databases

    STRINGi272634.MPN279.

    Structurei

    3D structure databases

    ProteinModelPortaliP75498.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 185182tr-type GAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0481.
    KOiK03596.
    OMAiCYTALEM.
    OrthoDBiEOG6ZKXQ4.

    Family and domain databases

    Gene3Di3.30.70.240. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPiMF_00071. LepA.
    InterProiIPR006297. EF-4.
    IPR000795. EF_GTP-bd_dom.
    IPR009022. EFG_III-V.
    IPR000640. EFG_V.
    IPR013842. LepA_GTP-bd_C.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view]
    PfamiPF00679. EFG_C. 1 hit.
    PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF06421. LepA_C. 1 hit.
    [Graphical view]
    PRINTSiPR00315. ELONGATNFCT.
    SMARTiSM00838. EFG_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF54980. SSF54980. 2 hits.
    TIGRFAMsiTIGR01393. lepA. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEiPS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P75498-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEQQKIRNFS IIAHIDHGKS TLSDRLIERS IGFEKRLLQA QMLDTMAIER    50
    ERGITIKLNA VQLKMAQGNQ QYLFHLVDTP GHVDFTYEVS RSLAACEGVL 100
    LLVDATQGIQ AQTISNTYLA LENNLEIIPV INKVDMESAD VEKTKQAFHQ 150
    LLGVDPNTIP LVSAKTGLGI DQLITTIIEK VPPPKGDESK PLKALLFDSY 200
    YDPYKGVVCF IRIFEGSLKL NDKIRFARSN SVYQIVELGI KNPFFEKQDV 250
    LKAGEIGWFS AGIKKLRDVT VGDTIVHAED TTTPPLPGYK KVLPMIYCGL 300
    YPIDNNDYQN LKMAMEKIIL SDVALEYEYE TSQALGFGVR CGFLGLLHMD 350
    VIKERLEREY NLKLISAPPS VRYKVLLTNG EELELDNPSL LPERSRIKSI 400
    SEPFVRVYID LPDHYLGTVI DLCQNFRGQY EKLEEIDIDR KRLVYLMPLG 450
    EIIYSFFDKL KSITKGYASL NYEFDQYQVS QLAKVEIMLN KQKVDALSFI 500
    AHHDFAFQRA KKFCVKLKEL IPKHLFEIPI QATIGSKVIA RETIKAVRKD 550
    VTAKLYGGDV TRKKKLLEKQ KEGKKRLKAI GSVELPQELF SHLLKDED 598
    Length:598
    Mass (Da):68,025
    Last modified:March 5, 2002 - v2
    Checksum:i4E5A1A230763B57C
    GO

    Sequence cautioni

    The sequence AAB96204.1 differs from that shown. Reason: Frameshift at position 540.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB96204.1. Frameshift.
    PIRiS73882.
    RefSeqiNP_109967.1. NC_000912.1.

    Genome annotation databases

    EnsemblBacteriaiAAB96204; AAB96204; MPN_279.
    GeneIDi876840.
    KEGGimpn:MPN279.
    PATRICi20021885. VBIMycPne110_0299.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB96204.1 . Frameshift.
    PIRi S73882.
    RefSeqi NP_109967.1. NC_000912.1.

    3D structure databases

    ProteinModelPortali P75498.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272634.MPN279.

    Proteomic databases

    PaxDbi P75498.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB96204 ; AAB96204 ; MPN_279 .
    GeneIDi 876840.
    KEGGi mpn:MPN279.
    PATRICi 20021885. VBIMycPne110_0299.

    Phylogenomic databases

    eggNOGi COG0481.
    KOi K03596.
    OMAi CYTALEM.
    OrthoDBi EOG6ZKXQ4.

    Enzyme and pathway databases

    BioCyci MPNE272634:GJ6Z-286-MONOMER.

    Family and domain databases

    Gene3Di 3.30.70.240. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPi MF_00071. LepA.
    InterProi IPR006297. EF-4.
    IPR000795. EF_GTP-bd_dom.
    IPR009022. EFG_III-V.
    IPR000640. EFG_V.
    IPR013842. LepA_GTP-bd_C.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view ]
    Pfami PF00679. EFG_C. 1 hit.
    PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF06421. LepA_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00315. ELONGATNFCT.
    SMARTi SM00838. EFG_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF54980. SSF54980. 2 hits.
    TIGRFAMsi TIGR01393. lepA. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEi PS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
      Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
      Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29342 / M129.
    2. Sinan C.
      Unpublished observations (FEB-2002)
      Cited for: IDENTIFICATION OF PROBABLE FRAMESHIFT.

    Entry informationi

    Entry nameiLEPA_MYCPN
    AccessioniPrimary (citable) accession number: P75498
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: March 5, 2002
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Mycoplasma pneumoniae
      Mycoplasma pneumoniae (strain M129): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3