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P75498 (LEPA_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Elongation factor 4

Short name=EF-4
EC=3.6.5.n1
Alternative name(s):
Ribosomal back-translocase LepA
Gene names
Name:lepA
Ordered Locus Names:MPN_279
ORF Names:MP556
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length598 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner By similarity. HAMAP-Rule MF_00071

Catalytic activity

GTP + H2O = GDP + phosphate. HAMAP-Rule MF_00071

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_00071.

Sequence similarities

Belongs to the GTP-binding elongation factor family. LepA subfamily.

Sequence caution

The sequence AAB96204.1 differs from that shown. Reason: Frameshift at position 540.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCell membrane
Membrane
   LigandGTP-binding
Nucleotide-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpositive regulation of translation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

ribosome binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

translation elongation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 598598Elongation factor 4 HAMAP-Rule MF_00071
PRO_0000176305

Regions

Nucleotide binding16 – 216GTP By similarity
Nucleotide binding132 – 1354GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
P75498 [UniParc].

Last modified March 5, 2002. Version 2.
Checksum: 4E5A1A230763B57C

FASTA59868,025
        10         20         30         40         50         60 
MEQQKIRNFS IIAHIDHGKS TLSDRLIERS IGFEKRLLQA QMLDTMAIER ERGITIKLNA 

        70         80         90        100        110        120 
VQLKMAQGNQ QYLFHLVDTP GHVDFTYEVS RSLAACEGVL LLVDATQGIQ AQTISNTYLA 

       130        140        150        160        170        180 
LENNLEIIPV INKVDMESAD VEKTKQAFHQ LLGVDPNTIP LVSAKTGLGI DQLITTIIEK 

       190        200        210        220        230        240 
VPPPKGDESK PLKALLFDSY YDPYKGVVCF IRIFEGSLKL NDKIRFARSN SVYQIVELGI 

       250        260        270        280        290        300 
KNPFFEKQDV LKAGEIGWFS AGIKKLRDVT VGDTIVHAED TTTPPLPGYK KVLPMIYCGL 

       310        320        330        340        350        360 
YPIDNNDYQN LKMAMEKIIL SDVALEYEYE TSQALGFGVR CGFLGLLHMD VIKERLEREY 

       370        380        390        400        410        420 
NLKLISAPPS VRYKVLLTNG EELELDNPSL LPERSRIKSI SEPFVRVYID LPDHYLGTVI 

       430        440        450        460        470        480 
DLCQNFRGQY EKLEEIDIDR KRLVYLMPLG EIIYSFFDKL KSITKGYASL NYEFDQYQVS 

       490        500        510        520        530        540 
QLAKVEIMLN KQKVDALSFI AHHDFAFQRA KKFCVKLKEL IPKHLFEIPI QATIGSKVIA 

       550        560        570        580        590 
RETIKAVRKD VTAKLYGGDV TRKKKLLEKQ KEGKKRLKAI GSVELPQELF SHLLKDED 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.
[2]Sinan C.
Unpublished observations (FEB-2002)
Cited for: IDENTIFICATION OF PROBABLE FRAMESHIFT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB96204.1. Frameshift.
PIRS73882.
RefSeqNP_109967.1. NC_000912.1.

3D structure databases

ProteinModelPortalP75498.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272634.MPN279.

Proteomic databases

PaxDbP75498.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB96204; AAB96204; MPN_279.
GeneID876840.
KEGGmpn:MPN279.
PATRIC20021885. VBIMycPne110_0299.

Phylogenomic databases

eggNOGCOG0481.
KOK03596.
OMACYTALEM.
OrthoDBEOG6ZKXQ4.

Enzyme and pathway databases

BioCycMPNE272634:GJ6Z-286-MONOMER.

Family and domain databases

Gene3D3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00071. LepA.
InterProIPR006297. EF-4.
IPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamPF00679. EFG_C. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SMARTSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEPA_MYCPN
AccessionPrimary (citable) accession number: P75498
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: March 5, 2002
Last modified: May 14, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names