P75498 (LEPA_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor 4
Short name=EF-4
EC=3.6.5.n1

Alternative name(s):
Ribosomal back-translocase LepA

Gene names

Name:	lepA
Ordered Locus Names:	MPN_279
ORF Names:	MP556

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma pneumoniae

Protein attributes

Sequence length	598 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner By similarity. HAMAP-Rule MF_00071
Catalytic activity	GTP + H2O = GDP + phosphate. HAMAP-Rule MF_00071
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_00071.
Sequence similarities	Belongs to the GTP-binding elongation factor family. LepA subfamily.
Sequence caution	The sequence AAB96204.1 differs from that shown. Reason: Frameshift at position 540.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC positive regulation of translation Inferred from electronic annotation. Source: HAMAP translational elongation Inferred from electronic annotation. Source: GOC
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: HAMAP ribosome binding Inferred from electronic annotation. Source: HAMAP translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 598	598	Elongation factor 4 HAMAP-Rule MF_00071		PRO_0000176305
Regions
	Nucleotide binding	16 – 21	6	GTP By similarity
	Nucleotide binding	132 – 135	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P75498 [UniParc]. Last modified March 5, 2002. Version 2. Checksum: 4E5A1A230763B57C	FASTA	598	68,025
	10 20 30 40 50 60 MEQQKIRNFS IIAHIDHGKS TLSDRLIERS IGFEKRLLQA QMLDTMAIER ERGITIKLNA 70 80 90 100 110 120 VQLKMAQGNQ QYLFHLVDTP GHVDFTYEVS RSLAACEGVL LLVDATQGIQ AQTISNTYLA 130 140 150 160 170 180 LENNLEIIPV INKVDMESAD VEKTKQAFHQ LLGVDPNTIP LVSAKTGLGI DQLITTIIEK 190 200 210 220 230 240 VPPPKGDESK PLKALLFDSY YDPYKGVVCF IRIFEGSLKL NDKIRFARSN SVYQIVELGI 250 260 270 280 290 300 KNPFFEKQDV LKAGEIGWFS AGIKKLRDVT VGDTIVHAED TTTPPLPGYK KVLPMIYCGL 310 320 330 340 350 360 YPIDNNDYQN LKMAMEKIIL SDVALEYEYE TSQALGFGVR CGFLGLLHMD VIKERLEREY 370 380 390 400 410 420 NLKLISAPPS VRYKVLLTNG EELELDNPSL LPERSRIKSI SEPFVRVYID LPDHYLGTVI 430 440 450 460 470 480 DLCQNFRGQY EKLEEIDIDR KRLVYLMPLG EIIYSFFDKL KSITKGYASL NYEFDQYQVS 490 500 510 520 530 540 QLAKVEIMLN KQKVDALSFI AHHDFAFQRA KKFCVKLKEL IPKHLFEIPI QATIGSKVIA 550 560 570 580 590 RETIKAVRKD VTAKLYGGDV TRKKKLLEKQ KEGKKRLKAI GSVELPQELF SHLLKDED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.
[2]	Sinan C. Unpublished observations (FEB-2002) Cited for: IDENTIFICATION OF PROBABLE FRAMESHIFT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB96204.1. Frameshift.
PIR	S73882.
RefSeq	NP_109967.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P75498.
ModBase	Search...
Protein-protein interaction databases
STRING	272634.MPN279.
Proteomic databases
PaxDb	P75498.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB96204; AAB96204; MPN_279.
GeneID	876840.
KEGG	mpn:MPN279.
PATRIC	20021885. VBIMycPne110_0299.
Phylogenomic databases
eggNOG	COG0481.
KO	K03596.
OMA	KCYGGDA.
ProtClustDB	PRK05433.
Enzyme and pathway databases
BioCyc	MPNE272634:GJ6Z-286-MONOMER.
Family and domain databases
Gene3D	3.30.70.240. 1 hit.
HAMAP	MF_00071. LepA.
InterPro	IPR006297. EF-4. IPR000795. EF_GTP-bd_dom. IPR009022. EFG_III-V. IPR000640. EFG_V. IPR013842. LepA_GTP-bd_C. IPR005225. Small_GTP-bd_dom. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF40. PTHR23115:SF40. 1 hit.
Pfam	PF00679. EFG_C. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF06421. LepA_C. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR01393. lepA. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEPA_MYCPN

Accession

Primary (citable) accession number: P75498

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	March 5, 2002
Last modified:	May 1, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program