P75484 (LSPA_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Synonyms:	lsp
Ordered Locus Names:	MPN_293
ORF Names:	MP542

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma pneumoniae

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP-Rule MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP-Rule MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP-Rule MF_00161
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MPN_387	P75395	1	EBI-2258812,EBI-2258802

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 184	184	Lipoprotein signal peptidase HAMAP-Rule MF_00161		PRO_0000178795
Regions
	Transmembrane	23 – 43	21	Helical; Potential
	Transmembrane	88 – 108	21	Helical; Potential
	Transmembrane	110 – 130	21	Helical; Potential
	Transmembrane	156 – 176	21	Helical; Potential
Sites
	Active site	131	1	By similarity
	Active site	157	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P75484 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: C29480B17C7B98A3	FASTA	184	20,586
	10 20 30 40 50 60 MAKAPTFFSK LLKQILFANR KPFLYYKLAL ILFVGFVILF QVFMLRAALN GEKGINGANG 70 80 90 100 110 120 TDVARSSFIS IYVIGNKGVG FSLLADQPGL VYFLQGFLSF IALFFLVFST SYNYIFWITT 130 140 150 160 170 180 LAFGSLGNFF DRLTSGSGEV LDYFVFSGGN SVFNLADCCI TFSFIGLFLS FLIQFFKEMK QTKS

References

[1]

"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB96190.1.
PIR	S73868.
RefSeq	NP_109981.1. NC_000912.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P75484. 1 interaction.
STRING	272634.MPN293.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB96190; AAB96190; MPN_293.
GeneID	877085.
KEGG	mpn:MPN293.
PATRIC	20021921. VBIMycPne110_0317.
Phylogenomic databases
eggNOG	COG0597.
KO	K03101.
OMA	YIFWITT.
ProtClustDB	PRK00573.
Enzyme and pathway databases
BioCyc	MPNE272634:GJ6Z-300-MONOMER.
UniPathway	UPA00665.
Family and domain databases
HAMAP	MF_00161. LspA.
InterPro	IPR001872. Peptidase_A8. [Graphical view]
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. lspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_MYCPN

Accession

Primary (citable) accession number: P75484

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program