Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lipoprotein signal peptidase

Gene

lspA

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein specifically catalyzes the removal of signal peptides from prolipoproteins.By similarity

Catalytic activityi

Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei131 – 1311By similarity
Active sitei157 – 1571By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BioCyciMPNE272634:GJ6Z-300-MONOMER.
UniPathwayiUPA00665.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoprotein signal peptidase (EC:3.4.23.36)
Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name:
SPase II
Gene namesi
Name:lspA
Synonyms:lsp
Ordered Locus Names:MPN_293
ORF Names:MP542
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000808 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei23 – 4321HelicalSequence AnalysisAdd
BLAST
Transmembranei88 – 10821HelicalSequence AnalysisAdd
BLAST
Transmembranei110 – 13021HelicalSequence AnalysisAdd
BLAST
Transmembranei156 – 17621HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 184184Lipoprotein signal peptidasePRO_0000178795Add
BLAST

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MPN_387P753951EBI-2258812,EBI-2258802

Protein-protein interaction databases

IntActiP75484. 1 interaction.
STRINGi272634.MPN293.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase A8 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0597.
KOiK03101.
OMAiYIFWITT.
OrthoDBiEOG647V1T.

Family and domain databases

HAMAPiMF_00161. LspA.
InterProiIPR001872. Peptidase_A8.
[Graphical view]
PfamiPF01252. Peptidase_A8. 1 hit.
[Graphical view]
PRINTSiPR00781. LIPOSIGPTASE.
TIGRFAMsiTIGR00077. lspA. 1 hit.
PROSITEiPS00855. SPASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P75484-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKAPTFFSK LLKQILFANR KPFLYYKLAL ILFVGFVILF QVFMLRAALN
60 70 80 90 100
GEKGINGANG TDVARSSFIS IYVIGNKGVG FSLLADQPGL VYFLQGFLSF
110 120 130 140 150
IALFFLVFST SYNYIFWITT LAFGSLGNFF DRLTSGSGEV LDYFVFSGGN
160 170 180
SVFNLADCCI TFSFIGLFLS FLIQFFKEMK QTKS
Length:184
Mass (Da):20,586
Last modified:February 1, 1997 - v1
Checksum:iC29480B17C7B98A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB96190.1.
PIRiS73868.
RefSeqiNP_109981.1. NC_000912.1.
WP_010874650.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB96190; AAB96190; MPN_293.
GeneIDi877085.
KEGGimpn:MPN293.
PATRICi20021921. VBIMycPne110_0317.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB96190.1.
PIRiS73868.
RefSeqiNP_109981.1. NC_000912.1.
WP_010874650.1. NC_000912.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP75484. 1 interaction.
STRINGi272634.MPN293.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB96190; AAB96190; MPN_293.
GeneIDi877085.
KEGGimpn:MPN293.
PATRICi20021921. VBIMycPne110_0317.

Phylogenomic databases

eggNOGiCOG0597.
KOiK03101.
OMAiYIFWITT.
OrthoDBiEOG647V1T.

Enzyme and pathway databases

UniPathwayiUPA00665.
BioCyciMPNE272634:GJ6Z-300-MONOMER.

Family and domain databases

HAMAPiMF_00161. LspA.
InterProiIPR001872. Peptidase_A8.
[Graphical view]
PfamiPF01252. Peptidase_A8. 1 hit.
[Graphical view]
PRINTSiPR00781. LIPOSIGPTASE.
TIGRFAMsiTIGR00077. lspA. 1 hit.
PROSITEiPS00855. SPASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
    Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
    Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29342 / M129.

Entry informationi

Entry nameiLSPA_MYCPN
AccessioniPrimary (citable) accession number: P75484
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 27, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.