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P75476

- PFKA_MYCPN

UniProt

P75476 - PFKA_MYCPN

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Protein

Probable ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.By similarity

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.

Cofactori

Mg2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei16 – 161ATP; via amide nitrogenBy similarity
Metal bindingi108 – 1081Magnesium; catalyticBy similarity
Active sitei132 – 1321Proton acceptorBy similarity
Binding sitei167 – 1671Substrate; shared with dimeric partnerBy similarity
Binding sitei226 – 2261SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi77 – 782ATPBy similarity
Nucleotide bindingi107 – 1104ATPBy similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-545.
MPNE272634:GJ6Z-309-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Synonyms:pfk
Ordered Locus Names:MPN_302
ORF Names:MP534
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000808: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Probable ATP-dependent 6-phosphofructokinasePRO_0000111965Add
BLAST

Proteomic databases

PaxDbiP75476.

Interactioni

Subunit structurei

Homodimer or homotetramer.By similarity

Protein-protein interaction databases

IntActiP75476. 2 interactions.
STRINGi272634.MPN302.

Structurei

3D structure databases

ProteinModelPortaliP75476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni130 – 1323Substrate bindingBy similarity
Regioni174 – 1763Substrate bindingBy similarity
Regioni258 – 2614Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
KOiK00850.
OMAiMELREGH.
OrthoDBiEOG644ZRM.

Family and domain databases

InterProiIPR012003. ATP_PFK_prok-type.
IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

P75476-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSPKTTKKIA ILTSGGDAPG MNATLVYLTR YATSSEIEVF FVKNGYYGLY
60 70 80 90 100
HDELVPAHQL DLSNSLFSAG TVIGSKRFVE FKELKVREQA AQNLKKRQID
110 120 130 140 150
YLVVIGGDGS YMGAKLLSEL GVNCYCLPGT IDNDINSSEF TIGFLTALES
160 170 180 190 200
IKVNVQAVYH TTKSHERVAI VEVMGRHCGD LAIFGALATN ADFVVTPSNK
210 220 230 240 250
MDLKQLESAV KKILQHQNHC VVIVSENIYG FDGYPSLTAI KQHFDANNMK
260 270 280 290 300
CNLVSLGHTQ RGFAPTSLEL VQISLMAQHT INLIGQNKVN QVIGNKANVP
310 320
VNYDFDQAFN MPPVDRSALI AVINKNII
Length:328
Mass (Da):35,989
Last modified:February 1, 1997 - v1
Checksum:iE29C9F0536766321
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB96182.1.
PIRiS73860.
RefSeqiNP_109990.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB96182; AAB96182; MPN_302.
GeneIDi877241.
KEGGimpn:MPN302.
PATRICi20021939. VBIMycPne110_0326.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB96182.1 .
PIRi S73860.
RefSeqi NP_109990.1. NC_000912.1.

3D structure databases

ProteinModelPortali P75476.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P75476. 2 interactions.
STRINGi 272634.MPN302.

Proteomic databases

PaxDbi P75476.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB96182 ; AAB96182 ; MPN_302 .
GeneIDi 877241.
KEGGi mpn:MPN302.
PATRICi 20021939. VBIMycPne110_0326.

Phylogenomic databases

eggNOGi COG0205.
KOi K00850.
OMAi MELREGH.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci MetaCyc:MONOMER-545.
MPNE272634:GJ6Z-309-MONOMER.

Family and domain databases

InterProi IPR012003. ATP_PFK_prok-type.
IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
    Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
    Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29342 / M129.

Entry informationi

Entry nameiPFKA_MYCPN
AccessioniPrimary (citable) accession number: P75476
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 26, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3