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Protein

Probable ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.By similarity

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.By similarity

Cofactori

Mg2+By similarity

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.By similarity
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. Probable ATP-dependent 6-phosphofructokinase (pfkA)
  4. Fructose-bisphosphate aldolase (fba)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei16ATP; via amide nitrogenBy similarity1
Metal bindingi108Magnesium; catalyticBy similarity1
Active sitei132Proton acceptorBy similarity1
Binding sitei167Substrate; shared with dimeric partnerBy similarity1
Binding sitei226SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 78ATPBy similarity2
Nucleotide bindingi107 – 110ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-545
MPNE272634:G1GJ3-472-MONOMER
UniPathwayiUPA00109; UER00182

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Synonyms:pfk
Ordered Locus Names:MPN_302
ORF Names:MP534
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000808 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

  • cytosol Source: AgBase

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001119651 – 328Probable ATP-dependent 6-phosphofructokinaseAdd BLAST328

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiP75476, 2 interactors

Structurei

3D structure databases

ProteinModelPortaliP75476
SMRiP75476
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni130 – 132Substrate bindingBy similarity3
Regioni174 – 176Substrate bindingBy similarity3
Regioni258 – 261Substrate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

KOiK00850
OMAiALYDLTF

Family and domain databases

InterProiView protein in InterPro
IPR022953 ATP_PFK
IPR012003 ATP_PFK_prok-type
IPR000023 Phosphofructokinase_dom
IPR035966 PKF_sf
PfamiView protein in Pfam
PF00365 PFK, 1 hit
PIRSFiPIRSF000532 ATP_PFK_prok, 1 hit
PRINTSiPR00476 PHFRCTKINASE
SUPFAMiSSF53784 SSF53784, 1 hit

Sequencei

Sequence statusi: Complete.

P75476-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPKTTKKIA ILTSGGDAPG MNATLVYLTR YATSSEIEVF FVKNGYYGLY
60 70 80 90 100
HDELVPAHQL DLSNSLFSAG TVIGSKRFVE FKELKVREQA AQNLKKRQID
110 120 130 140 150
YLVVIGGDGS YMGAKLLSEL GVNCYCLPGT IDNDINSSEF TIGFLTALES
160 170 180 190 200
IKVNVQAVYH TTKSHERVAI VEVMGRHCGD LAIFGALATN ADFVVTPSNK
210 220 230 240 250
MDLKQLESAV KKILQHQNHC VVIVSENIYG FDGYPSLTAI KQHFDANNMK
260 270 280 290 300
CNLVSLGHTQ RGFAPTSLEL VQISLMAQHT INLIGQNKVN QVIGNKANVP
310 320
VNYDFDQAFN MPPVDRSALI AVINKNII
Length:328
Mass (Da):35,989
Last modified:February 1, 1997 - v1
Checksum:iE29C9F0536766321
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA Translation: AAB96182.1
PIRiS73860
RefSeqiNP_109990.1, NC_000912.1
WP_010874659.1, NC_000912.1

Genome annotation databases

EnsemblBacteriaiAAB96182; AAB96182; MPN_302
GeneIDi877241
KEGGimpn:MPN302
PATRICifig|272634.6.peg.326

Similar proteinsi

Entry informationi

Entry nameiPFKA_MYCPN
AccessioniPrimary (citable) accession number: P75476
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health