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P75476 (PFKA_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable ATP-dependent 6-phosphofructokinase

Short name=ATP-PFK
Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Synonyms:pfk
Ordered Locus Names:MPN_302
ORF Names:MP534
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.

Cofactor

Magnesium By similarity.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Subunit structure

Homodimer or homotetramer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 328328Probable ATP-dependent 6-phosphofructokinase
PRO_0000111965

Regions

Nucleotide binding77 – 782ATP By similarity
Nucleotide binding107 – 1104ATP By similarity
Region130 – 1323Substrate binding By similarity
Region174 – 1763Substrate binding By similarity
Region258 – 2614Substrate binding By similarity

Sites

Active site1321Proton acceptor By similarity
Metal binding1081Magnesium; catalytic By similarity
Binding site161ATP; via amide nitrogen By similarity
Binding site1671Substrate; shared with dimeric partner By similarity
Binding site2261Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P75476 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: E29C9F0536766321

FASTA32835,989
        10         20         30         40         50         60 
MSPKTTKKIA ILTSGGDAPG MNATLVYLTR YATSSEIEVF FVKNGYYGLY HDELVPAHQL 

        70         80         90        100        110        120 
DLSNSLFSAG TVIGSKRFVE FKELKVREQA AQNLKKRQID YLVVIGGDGS YMGAKLLSEL 

       130        140        150        160        170        180 
GVNCYCLPGT IDNDINSSEF TIGFLTALES IKVNVQAVYH TTKSHERVAI VEVMGRHCGD 

       190        200        210        220        230        240 
LAIFGALATN ADFVVTPSNK MDLKQLESAV KKILQHQNHC VVIVSENIYG FDGYPSLTAI 

       250        260        270        280        290        300 
KQHFDANNMK CNLVSLGHTQ RGFAPTSLEL VQISLMAQHT INLIGQNKVN QVIGNKANVP 

       310        320 
VNYDFDQAFN MPPVDRSALI AVINKNII 

« Hide

References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB96182.1.
PIRS73860.
RefSeqNP_109990.1. NC_000912.1.

3D structure databases

ProteinModelPortalP75476.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP75476. 2 interactions.
STRING272634.MPN302.

Proteomic databases

PaxDbP75476.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB96182; AAB96182; MPN_302.
GeneID877241.
KEGGmpn:MPN302.
PATRIC20021939. VBIMycPne110_0326.

Phylogenomic databases

eggNOGCOG0205.
KOK00850.
OMAMELREGH.
OrthoDBEOG644ZRM.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-545.
MPNE272634:GJ6Z-309-MONOMER.
UniPathwayUPA00109; UER00182.

Family and domain databases

InterProIPR012003. ATP_PFK_prok.
IPR022953. Phosphofructokinase.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
ProtoNetSearch...

Entry information

Entry namePFKA_MYCPN
AccessionPrimary (citable) accession number: P75476
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: July 9, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names