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P75476

- PFKA_MYCPN

UniProt

P75476 - PFKA_MYCPN

Protein

Probable ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.By similarity

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.

    Cofactori

    Magnesium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei16 – 161ATP; via amide nitrogenBy similarity
    Metal bindingi108 – 1081Magnesium; catalyticBy similarity
    Active sitei132 – 1321Proton acceptorBy similarity
    Binding sitei167 – 1671Substrate; shared with dimeric partnerBy similarity
    Binding sitei226 – 2261SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi77 – 782ATPBy similarity
    Nucleotide bindingi107 – 1104ATPBy similarity

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-545.
    MPNE272634:GJ6Z-309-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
    Short name:
    ATP-PFK
    Short name:
    Phosphofructokinase
    Alternative name(s):
    Phosphohexokinase
    Gene namesi
    Name:pfkA
    Synonyms:pfk
    Ordered Locus Names:MPN_302
    ORF Names:MP534
    OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
    Taxonomic identifieri272634 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000808: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 328328Probable ATP-dependent 6-phosphofructokinasePRO_0000111965Add
    BLAST

    Proteomic databases

    PaxDbiP75476.

    Interactioni

    Subunit structurei

    Homodimer or homotetramer.By similarity

    Protein-protein interaction databases

    IntActiP75476. 2 interactions.
    STRINGi272634.MPN302.

    Structurei

    3D structure databases

    ProteinModelPortaliP75476.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni130 – 1323Substrate bindingBy similarity
    Regioni174 – 1763Substrate bindingBy similarity
    Regioni258 – 2614Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0205.
    KOiK00850.
    OMAiMELREGH.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    InterProiIPR012003. ATP_PFK_prok.
    IPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P75476-1 [UniParc]FASTAAdd to Basket

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    MSPKTTKKIA ILTSGGDAPG MNATLVYLTR YATSSEIEVF FVKNGYYGLY    50
    HDELVPAHQL DLSNSLFSAG TVIGSKRFVE FKELKVREQA AQNLKKRQID 100
    YLVVIGGDGS YMGAKLLSEL GVNCYCLPGT IDNDINSSEF TIGFLTALES 150
    IKVNVQAVYH TTKSHERVAI VEVMGRHCGD LAIFGALATN ADFVVTPSNK 200
    MDLKQLESAV KKILQHQNHC VVIVSENIYG FDGYPSLTAI KQHFDANNMK 250
    CNLVSLGHTQ RGFAPTSLEL VQISLMAQHT INLIGQNKVN QVIGNKANVP 300
    VNYDFDQAFN MPPVDRSALI AVINKNII 328
    Length:328
    Mass (Da):35,989
    Last modified:February 1, 1997 - v1
    Checksum:iE29C9F0536766321
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB96182.1.
    PIRiS73860.
    RefSeqiNP_109990.1. NC_000912.1.

    Genome annotation databases

    EnsemblBacteriaiAAB96182; AAB96182; MPN_302.
    GeneIDi877241.
    KEGGimpn:MPN302.
    PATRICi20021939. VBIMycPne110_0326.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB96182.1 .
    PIRi S73860.
    RefSeqi NP_109990.1. NC_000912.1.

    3D structure databases

    ProteinModelPortali P75476.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P75476. 2 interactions.
    STRINGi 272634.MPN302.

    Proteomic databases

    PaxDbi P75476.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB96182 ; AAB96182 ; MPN_302 .
    GeneIDi 877241.
    KEGGi mpn:MPN302.
    PATRICi 20021939. VBIMycPne110_0326.

    Phylogenomic databases

    eggNOGi COG0205.
    KOi K00850.
    OMAi MELREGH.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci MetaCyc:MONOMER-545.
    MPNE272634:GJ6Z-309-MONOMER.

    Family and domain databases

    InterProi IPR012003. ATP_PFK_prok.
    IPR022953. Phosphofructokinase.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
      Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
      Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29342 / M129.

    Entry informationi

    Entry nameiPFKA_MYCPN
    AccessioniPrimary (citable) accession number: P75476
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Mycoplasma pneumoniae
      Mycoplasma pneumoniae (strain M129): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3