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Protein

Probable ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.By similarity

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.By similarity

Cofactori

Mg2+By similarity

Pathwayi: glycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.By similarity
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. Probable ATP-dependent 6-phosphofructokinase (pfkA)
  4. Fructose-bisphosphate aldolase (fba)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei16ATP; via amide nitrogenBy similarity1
Metal bindingi108Magnesium; catalyticBy similarity1
Active sitei132Proton acceptorBy similarity1
Binding sitei167Substrate; shared with dimeric partnerBy similarity1
Binding sitei226SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 78ATPBy similarity2
Nucleotide bindingi107 – 110ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-545.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Synonyms:pfk
Ordered Locus Names:MPN_302
ORF Names:MP534
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000808 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001119651 – 328Probable ATP-dependent 6-phosphofructokinaseAdd BLAST328

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiP75476. 2 interactors.

Structurei

3D structure databases

ProteinModelPortaliP75476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni130 – 132Substrate bindingBy similarity3
Regioni174 – 176Substrate bindingBy similarity3
Regioni258 – 261Substrate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

KOiK00850.
OMAiEGRSNAI.

Family and domain databases

InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

P75476-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPKTTKKIA ILTSGGDAPG MNATLVYLTR YATSSEIEVF FVKNGYYGLY
60 70 80 90 100
HDELVPAHQL DLSNSLFSAG TVIGSKRFVE FKELKVREQA AQNLKKRQID
110 120 130 140 150
YLVVIGGDGS YMGAKLLSEL GVNCYCLPGT IDNDINSSEF TIGFLTALES
160 170 180 190 200
IKVNVQAVYH TTKSHERVAI VEVMGRHCGD LAIFGALATN ADFVVTPSNK
210 220 230 240 250
MDLKQLESAV KKILQHQNHC VVIVSENIYG FDGYPSLTAI KQHFDANNMK
260 270 280 290 300
CNLVSLGHTQ RGFAPTSLEL VQISLMAQHT INLIGQNKVN QVIGNKANVP
310 320
VNYDFDQAFN MPPVDRSALI AVINKNII
Length:328
Mass (Da):35,989
Last modified:February 1, 1997 - v1
Checksum:iE29C9F0536766321
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB96182.1.
PIRiS73860.
RefSeqiNP_109990.1. NC_000912.1.
WP_010874659.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB96182; AAB96182; MPN_302.
GeneIDi877241.
KEGGimpn:MPN302.
PATRICi20021939. VBIMycPne110_0326.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB96182.1.
PIRiS73860.
RefSeqiNP_109990.1. NC_000912.1.
WP_010874659.1. NC_000912.1.

3D structure databases

ProteinModelPortaliP75476.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP75476. 2 interactors.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB96182; AAB96182; MPN_302.
GeneIDi877241.
KEGGimpn:MPN302.
PATRICi20021939. VBIMycPne110_0326.

Phylogenomic databases

KOiK00850.
OMAiEGRSNAI.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
BioCyciMetaCyc:MONOMER-545.

Family and domain databases

InterProiIPR022953. ATP_PFK.
IPR012003. ATP_PFK_prok-type.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPFKA_MYCPN
AccessioniPrimary (citable) accession number: P75476
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.