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Protein

Ribonucleoside-diphosphate reductase subunit beta

Gene

nrdF

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi87 – 871Iron 1By similarity
Metal bindingi121 – 1211Iron 1By similarity
Active sitei125 – 1251By similarity
Metal bindingi215 – 2151Iron 2

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
  2. deoxyribonucleotide biosynthetic process Source: InterPro
  3. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMPNE272634:GJ6Z-339-MONOMER.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit beta (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small subunit
Gene namesi
Name:nrdF
Ordered Locus Names:MPN_322
ORF Names:MP514
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000808 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 339339Ribonucleoside-diphosphate reductase subunit betaPRO_0000190484Add
BLAST

Proteomic databases

PaxDbiP75461.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

IntActiP75461. 4 interactions.
STRINGi272634.MPN322.

Structurei

3D structure databases

ProteinModelPortaliP75461.
SMRiP75461. Positions 24-307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0208.
KOiK00526.
OMAiYYQADEP.
OrthoDBiEOG68DD19.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR026494. RNR_NrdF.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
TIGRFAMsiTIGR04171. RNR_1b_NrdF. 1 hit.

Sequencei

Sequence statusi: Complete.

P75461-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANTKKYFLE SVSPLEYAQK KFQGNLRSVN WNLVDDEKDL EVWNRITQNF
60 70 80 90 100
WLPEKIPVSN DIPSWKQLSK EWQDLITKTF TGLTLLDTIQ ATIGDIKQID
110 120 130 140 150
YALTDHEQVI YANFAFMVGV HARSYGTIFS TLCTSEQITE AHEWVVKTES
160 170 180 190 200
LQKRAKALIP YYTGKDPLKS KVAAALMPGF LLYGGFYLPF YLSSRKQLPN
210 220 230 240 250
TSDIIRLILR DKVIHNYYSG YKFQRKVEKM SKEKQAEMKR FVFDLMYELI
260 270 280 290 300
ELEKAYLKEL YEGFGIVEDA IKFSIYNAGK FLQNLGYDSP FTEEETRIKP
310 320 330
EIFAQLSARA DENHDFFSGN GSSYVMGISE ETEDKDWDF
Length:339
Mass (Da):39,413
Last modified:February 1, 1997 - v1
Checksum:iAE2FA78C2B745176
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB96162.1.
PIRiS73840.
RefSeqiNP_110010.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB96162; AAB96162; MPN_322.
GeneIDi876942.
KEGGimpn:MPN322.
PATRICi20021998. VBIMycPne110_0346.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB96162.1.
PIRiS73840.
RefSeqiNP_110010.1. NC_000912.1.

3D structure databases

ProteinModelPortaliP75461.
SMRiP75461. Positions 24-307.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP75461. 4 interactions.
STRINGi272634.MPN322.

Proteomic databases

PaxDbiP75461.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB96162; AAB96162; MPN_322.
GeneIDi876942.
KEGGimpn:MPN322.
PATRICi20021998. VBIMycPne110_0346.

Phylogenomic databases

eggNOGiCOG0208.
KOiK00526.
OMAiYYQADEP.
OrthoDBiEOG68DD19.

Enzyme and pathway databases

UniPathwayiUPA00326.
BioCyciMPNE272634:GJ6Z-339-MONOMER.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR026494. RNR_NrdF.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
TIGRFAMsiTIGR04171. RNR_1b_NrdF. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
    Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
    Nucleic Acids Res. 24:4420-4449(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29342 / M129.

Entry informationi

Entry nameiRIR2_MYCPN
AccessioniPrimary (citable) accession number: P75461
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 7, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Seems to lack two of the iron-binding residues.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.