P75461 (RIR2_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonucleoside-diphosphate reductase subunit beta
EC=1.17.4.1

Alternative name(s):
Ribonucleotide reductase small subunit

Gene names

Name:	nrdF
Ordered Locus Names:	MPN_322
ORF Names:	MP514

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma pneumoniae

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase small chain family.
Caution	Seems to lack two of the iron-binding residues.

Ontologies

Keywords
Biological process	DNA replication
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-UniPathway deoxyribonucleoside diphosphate metabolic process Inferred from electronic annotation. Source: InterPro deoxyribonucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Ribonucleoside-diphosphate reductase subunit beta		PRO_0000190484
Sites
	Active site	125	1	By similarity
	Metal binding	87	1	Iron 1 By similarity
	Metal binding	121	1	Iron 1 By similarity
	Metal binding	215	1	Iron 2

Sequences

	Sequence		Length	Mass (Da)
	P75461 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: AE2FA78C2B745176	FASTA	339	39,413
	10 20 30 40 50 60 MANTKKYFLE SVSPLEYAQK KFQGNLRSVN WNLVDDEKDL EVWNRITQNF WLPEKIPVSN 70 80 90 100 110 120 DIPSWKQLSK EWQDLITKTF TGLTLLDTIQ ATIGDIKQID YALTDHEQVI YANFAFMVGV 130 140 150 160 170 180 HARSYGTIFS TLCTSEQITE AHEWVVKTES LQKRAKALIP YYTGKDPLKS KVAAALMPGF 190 200 210 220 230 240 LLYGGFYLPF YLSSRKQLPN TSDIIRLILR DKVIHNYYSG YKFQRKVEKM SKEKQAEMKR 250 260 270 280 290 300 FVFDLMYELI ELEKAYLKEL YEGFGIVEDA IKFSIYNAGK FLQNLGYDSP FTEEETRIKP 310 320 330 EIFAQLSARA DENHDFFSGN GSSYVMGISE ETEDKDWDF

References

[1]

"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB96162.1.
PIR	S73840.
RefSeq	NP_110010.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P75461.
SMR	P75461. Positions 24-307.
ModBase	Search...
Protein-protein interaction databases
IntAct	P75461. 4 interactions.
STRING	272634.MPN322.
Proteomic databases
PaxDb	P75461.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB96162; AAB96162; MPN_322.
GeneID	876942.
KEGG	mpn:MPN322.
PATRIC	20021998. VBIMycPne110_0346.
Phylogenomic databases
eggNOG	COG0208.
KO	K00526.
OMA	SGYKFQR.
ProtClustDB	PRK09614.
Enzyme and pathway databases
BioCyc	MPNE272634:GJ6Z-339-MONOMER.
UniPathway	UPA00326.
Family and domain databases
Gene3D	1.10.620.20. 1 hit.
InterPro	IPR009078. Ferritin-like_SF. IPR012348. RNR-rel. IPR026494. RNR_1b_NrdF. IPR000358. RNR_small. [Graphical view]
PANTHER	PTHR23409. PTHR23409. 1 hit.
Pfam	PF00268. Ribonuc_red_sm. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
TIGRFAMs	TIGR04171. RNR_1b_NrdF. 1 hit.
PROSITE	PS00368. RIBORED_SMALL. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR2_MYCPN

Accession

Primary (citable) accession number: P75461

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 29, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program