Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative type I restriction enzyme MpnORFDP M protein

Gene

MPN_342

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Methylation of specific adenine residues; required for both restriction and modification activities.By similarity

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei265 – 2651S-adenosyl-L-methionineBy similarity

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMPNE272634:GJ6Z-359-MONOMER.

Protein family/group databases

REBASEi6703. M.MpnII.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative type I restriction enzyme MpnORFDP M protein (EC:2.1.1.72)
Short name:
M.MpnORFDP
Gene namesi
Ordered Locus Names:MPN_342
ORF Names:H91_orf543, MP494
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000808: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 543543Putative type I restriction enzyme MpnORFDP M proteinPRO_0000088028Add
BLAST

Proteomic databases

PaxDbiP75436.

Interactioni

Subunit structurei

The type I restriction/modification system is composed of three polypeptides R, M and S.

Protein-protein interaction databases

IntActiP75436. 2 interactions.
STRINGi272634.MPN342.

Structurei

3D structure databases

ProteinModelPortaliP75436.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni208 – 2136S-adenosyl-L-methionine bindingBy similarity
Regioni240 – 2423S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0286.
KOiK03427.
OMAiWKIANEV.
OrthoDBiEOG64JFNB.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR022749. D12N6_MeTrfase_N.
IPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR004546. Restrct_endonuc_typeI_HsdM.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF12161. HsdM_N. 1 hit.
PF02384. N6_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00497. hsdM. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P75436-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEKKRTEQRN GVEKKIWEIA DKLRGTIDGW DFKSYVLIGL FYRFLSENLC
60 70 80 90 100
KYFNDSERRN NPDFSYENLT DDYEAIDALK DAAIASKGFF IKPSQLFQNV
110 120 130 140 150
VKSIRENKNN EDLNTTLRDI FDDIEKSTEL GDGRSKESFK GLFKDFNVSE
160 170 180 190 200
VKLGSTLTIR TEKLKELLTS IDTMELDEFE KNSIDAFGDA YEFLISMYAQ
210 220 230 240 250
NAGKSGGEFF TPQDISELLA RIAIGKKDTV DDVYDMACGS GSLLLQVIKV
260 270 280 290 300
LGKEKTSLVS YYGQEINHTT YNLCRMNMIL HNIDYANFNI INADTLTTKE
310 320 330 340 350
WEKHYVNCSN ENGFEVVVSN PPYSISWAGD KKSNLVSDVR FKDAGTLAPN
360 370 380 390 400
SKADLAFVLH ALYVLGQEGT AAIVCFPGIL YREGKEQTIR KYLVDQNFVD
410 420 430 440 450
AVIQLPSNLF STTSIATSIL VLKKNRDKKD PIFFIDGSNE FVREKKNNRL
460 470 480 490 500
SPKNIEKIVD CFNSKKEEAN FAKSVERDKI RESNYDLTVG KYVNSEAEKE
510 520 530 540
ELDIKVLNHS IDEIVDKQKD LRTKIKDIIQ DIKVDFDNID INN
Length:543
Mass (Da):61,975
Last modified:February 1, 1997 - v1
Checksum:i618EE22B4FE03064
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB96142.1.
PIRiS73820.
RefSeqiNP_110030.1. NC_000912.1.
WP_010874698.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB96142; AAB96142; MPN_342.
GeneIDi876775.
KEGGimpn:MPN342.
PATRICi20022038. VBIMycPne110_0366.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB96142.1.
PIRiS73820.
RefSeqiNP_110030.1. NC_000912.1.
WP_010874698.1. NC_000912.1.

3D structure databases

ProteinModelPortaliP75436.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP75436. 2 interactions.
STRINGi272634.MPN342.

Protein family/group databases

REBASEi6703. M.MpnII.

Proteomic databases

PaxDbiP75436.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB96142; AAB96142; MPN_342.
GeneIDi876775.
KEGGimpn:MPN342.
PATRICi20022038. VBIMycPne110_0366.

Phylogenomic databases

eggNOGiCOG0286.
KOiK03427.
OMAiWKIANEV.
OrthoDBiEOG64JFNB.

Enzyme and pathway databases

BioCyciMPNE272634:GJ6Z-359-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR022749. D12N6_MeTrfase_N.
IPR003356. DNA_methylase_A-5.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR004546. Restrct_endonuc_typeI_HsdM.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF12161. HsdM_N. 1 hit.
PF02384. N6_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00497. hsdM. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
    Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
    Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29342 / M129.

Entry informationi

Entry nameiT1MD_MYCPN
AccessioniPrimary (citable) accession number: P75436
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: February 1, 1997
Last modified: February 4, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.