P75436 (T1MD_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative type I restriction enzyme MpnORFDP M protein
Short name=M.MpnORFDP
EC=2.1.1.72

Gene names

Ordered Locus Names:	MPN_342
ORF Names:	H91_orf543, MP494

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma pneumoniae

Protein attributes

Sequence length	543 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Methylation of specific adenine residues; required for both restriction and modification activities By similarity.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Subunit structure	The type I restriction/modification system is composed of three polypeptides R, M and S.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 543	543	Putative type I restriction enzyme MpnORFDP M protein		PRO_0000088028
Regions
	Region	208 – 213	6	S-adenosyl-L-methionine binding By similarity
	Region	240 – 242	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	265	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P75436 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 618EE22B4FE03064	FASTA	543	61,975
	10 20 30 40 50 60 MEKKRTEQRN GVEKKIWEIA DKLRGTIDGW DFKSYVLIGL FYRFLSENLC KYFNDSERRN 70 80 90 100 110 120 NPDFSYENLT DDYEAIDALK DAAIASKGFF IKPSQLFQNV VKSIRENKNN EDLNTTLRDI 130 140 150 160 170 180 FDDIEKSTEL GDGRSKESFK GLFKDFNVSE VKLGSTLTIR TEKLKELLTS IDTMELDEFE 190 200 210 220 230 240 KNSIDAFGDA YEFLISMYAQ NAGKSGGEFF TPQDISELLA RIAIGKKDTV DDVYDMACGS 250 260 270 280 290 300 GSLLLQVIKV LGKEKTSLVS YYGQEINHTT YNLCRMNMIL HNIDYANFNI INADTLTTKE 310 320 330 340 350 360 WEKHYVNCSN ENGFEVVVSN PPYSISWAGD KKSNLVSDVR FKDAGTLAPN SKADLAFVLH 370 380 390 400 410 420 ALYVLGQEGT AAIVCFPGIL YREGKEQTIR KYLVDQNFVD AVIQLPSNLF STTSIATSIL 430 440 450 460 470 480 VLKKNRDKKD PIFFIDGSNE FVREKKNNRL SPKNIEKIVD CFNSKKEEAN FAKSVERDKI 490 500 510 520 530 540 RESNYDLTVG KYVNSEAEKE ELDIKVLNHS IDEIVDKQKD LRTKIKDIIQ DIKVDFDNID INN

References

[1]

"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB96142.1.
PIR	S73820.
RefSeq	NP_110030.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P75436.
ModBase	Search...
Protein-protein interaction databases
IntAct	P75436. 2 interactions.
STRING	272634.MPN342.
Protein family/group databases
REBASE	6703. M.MpnII.
Proteomic databases
PaxDb	P75436.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB96142; AAB96142; MPN_342.
GeneID	876775.
KEGG	mpn:MPN342.
PATRIC	20022038. VBIMycPne110_0366.
Phylogenomic databases
eggNOG	COG0286.
KO	K03427.
OMA	EFFTPQH.
Enzyme and pathway databases
BioCyc	MPNE272634:GJ6Z-359-MONOMER.
Family and domain databases
InterPro	IPR022749. D12N6_MeTrfase_N. IPR003356. DNA_methylase_A-5. IPR002052. DNA_methylase_N6_adenine_CS. IPR002296. N12N6_MeTrfase. IPR004546. Restrct_endonuc_typeI_HsdM. [Graphical view]
Pfam	PF12161. HsdM_N. 1 hit. PF02384. N6_Mtase. 1 hit. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
TIGRFAMs	TIGR00497. hsdM. 1 hit.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T1MD_MYCPN

Accession

Primary (citable) accession number: P75436

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program