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P75430

- MTHFS_MYCPN

UniProt

P75430 - MTHFS_MYCPN

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Protein

5-formyltetrahydrofolate cyclo-ligase

Gene

MPN_348

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in folate metabolism. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-FTHF) to yield 5,10-methenyltetrahydrofolate.1 Publication

Catalytic activityi

ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate.1 Publication

Cofactori

Mg2+1 Publication, Mn2+1 Publication, Ca2+1 Publication, Zn2+1 Publication, Fe2+1 Publication, Co2+1 Publication, Cu2+1 PublicationNote: Magnesium. It can also use divalent cations such as manganese, calcium, zinc, iron, cobalt and copper.1 Publication

Kineticsi

Kcat is 0.94 sec(-1) for cyclo-ligase activity with 5-FTHF (at pH 6 and 37 degrees Celsius). Kcat is 0.99 sec(-1) for cyclo-ligase activity with ATP (at pH 6 and 37 degrees Celsius).

  1. KM=165 µM for 5-FTHF (at pH 6 and 37 degrees Celsius)1 Publication
  2. KM=166 µM for ATP (at pH 6 and 37 degrees Celsius)1 Publication

Vmax=2.70 µmol/min/mg enzyme toward 5-FTHF (at pH 6 and 37 degrees Celsius)1 Publication

Vmax=2.84 µmol/min/mg enzyme toward ATP (at pH 6 and 37 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501Substrate1 Publication
Binding sitei55 – 551Substrate1 Publication
Metal bindingi124 – 1241Magnesium1 Publication
Binding sitei125 – 1251ATP
Binding sitei153 – 1531ATP
Metal bindingi154 – 1541Magnesium1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi3 – 75ATP
Nucleotide bindingi115 – 1239ATPCurated

GO - Molecular functioni

  1. 5-formyltetrahydrofolate cyclo-ligase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMPNE272634:GJ6Z-365-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
5-formyltetrahydrofolate cyclo-ligase (EC:6.3.3.2)
Alternative name(s):
5,10-methenyl-tetrahydrofolate synthetase
Short name:
MTHFS
Short name:
Methenyl-THF synthetase
Gene namesi
Ordered Locus Names:MPN_348
ORF Names:H91_orf164, MP488
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000808: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1641645-formyltetrahydrofolate cyclo-ligasePRO_0000200288Add
BLAST

Interactioni

Subunit structurei

Monomer or homodimer.2 Publications

Protein-protein interaction databases

STRINGi272634.MPN348.

Structurei

Secondary structure

1
164
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1614Combined sources
Helixi19 – 3719Combined sources
Beta strandi45 – 473Combined sources
Helixi60 – 689Combined sources
Beta strandi72 – 787Combined sources
Beta strandi80 – 823Combined sources
Beta strandi84 – 874Combined sources
Helixi96 – 983Combined sources
Beta strandi101 – 1055Combined sources
Beta strandi107 – 1093Combined sources
Beta strandi114 – 1163Combined sources
Beta strandi118 – 1203Combined sources
Helixi122 – 1265Combined sources
Helixi127 – 1293Combined sources
Beta strandi136 – 1405Combined sources
Helixi142 – 1443Combined sources
Beta strandi158 – 1625Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1SBQX-ray2.20A/B1-164[»]
1U3FX-ray2.50A/B1-164[»]
1U3GX-ray2.50A1-164[»]
ProteinModelPortaliP75430.
SMRiP75430. Positions 1-164.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP75430.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0212.
OMAiKANHRIG.
OrthoDBiEOG6RVG1P.

Family and domain databases

Gene3Di3.40.50.10420. 1 hit.
InterProiIPR002698. FTHF_cligase.
IPR024185. FTHF_cligase-like.
[Graphical view]
PANTHERiPTHR23407:SF1. PTHR23407:SF1. 1 hit.
PfamiPF01812. 5-FTHF_cyc-lig. 1 hit.
[Graphical view]
PIRSFiPIRSF006806. FTHF_cligase. 1 hit.
TIGRFAMsiTIGR02727. MTHFS_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

P75430-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDKNALRKQI LQKRMALSTI EKSHLDQKIN QKLVAFLTPK PCIKTIALYE
60 70 80 90 100
PIKNEVTFVD FFFEFLKINQ IRAVYPKVIS DTEIIFIDQE TNTFEPNQID
110 120 130 140 150
CFLIPLVGFN KDNYRLGFGK GYYDRYLMQL TRQQPKIGIA YSFQKGDFLA
160
DPWDVQLDLI INDE
Length:164
Mass (Da):19,265
Last modified:February 1, 1997 - v1
Checksum:iD44365FDE62A9DDF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB96136.1.
PIRiS73814.
RefSeqiNP_110036.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB96136; AAB96136; MPN_348.
GeneIDi876747.
KEGGimpn:MPN348.
PATRICi20022056. VBIMycPne110_0375.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB96136.1 .
PIRi S73814.
RefSeqi NP_110036.1. NC_000912.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1SBQ X-ray 2.20 A/B 1-164 [» ]
1U3F X-ray 2.50 A/B 1-164 [» ]
1U3G X-ray 2.50 A 1-164 [» ]
ProteinModelPortali P75430.
SMRi P75430. Positions 1-164.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272634.MPN348.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB96136 ; AAB96136 ; MPN_348 .
GeneIDi 876747.
KEGGi mpn:MPN348.
PATRICi 20022056. VBIMycPne110_0375.

Phylogenomic databases

eggNOGi COG0212.
OMAi KANHRIG.
OrthoDBi EOG6RVG1P.

Enzyme and pathway databases

BioCyci MPNE272634:GJ6Z-365-MONOMER.

Miscellaneous databases

EvolutionaryTracei P75430.

Family and domain databases

Gene3Di 3.40.50.10420. 1 hit.
InterProi IPR002698. FTHF_cligase.
IPR024185. FTHF_cligase-like.
[Graphical view ]
PANTHERi PTHR23407:SF1. PTHR23407:SF1. 1 hit.
Pfami PF01812. 5-FTHF_cyc-lig. 1 hit.
[Graphical view ]
PIRSFi PIRSF006806. FTHF_cligase. 1 hit.
TIGRFAMsi TIGR02727. MTHFS_bact. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
    Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
    Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29342 / M129.
  2. "Crystal structure of methenyltetrahydrofolate synthetase from Mycoplasma pneumoniae (GI: 13508087) at 2.2 A resolution."
    Chen S., Shin D.-H., Pufan R., Kim R., Kim S.H.
    Proteins 56:839-843(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS), SUBUNIT.
  3. "Structural and functional characterization of a 5,10-methenyltetrahydrofolate synthetase from Mycoplasma pneumoniae (GI: 13508087)."
    Chen S., Yakunin A.F., Proudfoot M., Kim R., Kim S.H.
    Proteins 61:433-443(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEX WITH SUBSTRATE; ADP AND MAGNESIUM, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, SUBUNIT.

Entry informationi

Entry nameiMTHFS_MYCPN
AccessioniPrimary (citable) accession number: P75430
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 26, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3