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P75430

- MTHFS_MYCPN

UniProt

P75430 - MTHFS_MYCPN

Protein

5-formyltetrahydrofolate cyclo-ligase

Gene

MPN_348

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Involved in folate metabolism. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-FTHF) to yield 5,10-methenyltetrahydrofolate.1 Publication

    Catalytic activityi

    ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate.1 Publication

    Cofactori

    Magnesium. It can also use divalent cations such as manganese, calcium, zinc, iron, cobalt and copper.1 Publication

    Kineticsi

    Kcat is 0.94 sec(-1) for cyclo-ligase activity with 5-FTHF (at pH 6 and 37 degrees Celsius). Kcat is 0.99 sec(-1) for cyclo-ligase activity with ATP (at pH 6 and 37 degrees Celsius).

    1. KM=165 µM for 5-FTHF (at pH 6 and 37 degrees Celsius)1 Publication
    2. KM=166 µM for ATP (at pH 6 and 37 degrees Celsius)1 Publication

    Vmax=2.70 µmol/min/mg enzyme toward 5-FTHF (at pH 6 and 37 degrees Celsius)1 Publication

    Vmax=2.84 µmol/min/mg enzyme toward ATP (at pH 6 and 37 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei50 – 501Substrate1 Publication
    Binding sitei55 – 551Substrate1 Publication
    Metal bindingi124 – 1241Magnesium1 Publication
    Binding sitei125 – 1251ATP
    Binding sitei153 – 1531ATP
    Metal bindingi154 – 1541Magnesium1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi3 – 75ATP
    Nucleotide bindingi115 – 1239ATPCurated

    GO - Molecular functioni

    1. 5-formyltetrahydrofolate cyclo-ligase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. folic acid-containing compound biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMPNE272634:GJ6Z-365-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-formyltetrahydrofolate cyclo-ligase (EC:6.3.3.2)
    Alternative name(s):
    5,10-methenyl-tetrahydrofolate synthetase
    Short name:
    MTHFS
    Short name:
    Methenyl-THF synthetase
    Gene namesi
    Ordered Locus Names:MPN_348
    ORF Names:H91_orf164, MP488
    OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
    Taxonomic identifieri272634 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000808: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 1641645-formyltetrahydrofolate cyclo-ligasePRO_0000200288Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer or homodimer.2 Publications

    Protein-protein interaction databases

    STRINGi272634.MPN348.

    Structurei

    Secondary structure

    1
    164
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 1614
    Helixi19 – 3719
    Beta strandi45 – 473
    Helixi60 – 689
    Beta strandi72 – 787
    Beta strandi80 – 823
    Beta strandi84 – 874
    Helixi96 – 983
    Beta strandi101 – 1055
    Beta strandi107 – 1093
    Beta strandi114 – 1163
    Beta strandi118 – 1203
    Helixi122 – 1265
    Helixi127 – 1293
    Beta strandi136 – 1405
    Helixi142 – 1443
    Beta strandi158 – 1625

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1SBQX-ray2.20A/B1-164[»]
    1U3FX-ray2.50A/B1-164[»]
    1U3GX-ray2.50A1-164[»]
    ProteinModelPortaliP75430.
    SMRiP75430. Positions 1-164.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP75430.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0212.
    OMAiKANHRIG.
    OrthoDBiEOG6RVG1P.

    Family and domain databases

    Gene3Di3.40.50.10420. 1 hit.
    InterProiIPR002698. FTHF_cligase.
    IPR024185. FTHF_cligase-like.
    [Graphical view]
    PANTHERiPTHR23407:SF1. PTHR23407:SF1. 1 hit.
    PfamiPF01812. 5-FTHF_cyc-lig. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006806. FTHF_cligase. 1 hit.
    TIGRFAMsiTIGR02727. MTHFS_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P75430-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDKNALRKQI LQKRMALSTI EKSHLDQKIN QKLVAFLTPK PCIKTIALYE    50
    PIKNEVTFVD FFFEFLKINQ IRAVYPKVIS DTEIIFIDQE TNTFEPNQID 100
    CFLIPLVGFN KDNYRLGFGK GYYDRYLMQL TRQQPKIGIA YSFQKGDFLA 150
    DPWDVQLDLI INDE 164
    Length:164
    Mass (Da):19,265
    Last modified:February 1, 1997 - v1
    Checksum:iD44365FDE62A9DDF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB96136.1.
    PIRiS73814.
    RefSeqiNP_110036.1. NC_000912.1.

    Genome annotation databases

    EnsemblBacteriaiAAB96136; AAB96136; MPN_348.
    GeneIDi876747.
    KEGGimpn:MPN348.
    PATRICi20022056. VBIMycPne110_0375.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB96136.1 .
    PIRi S73814.
    RefSeqi NP_110036.1. NC_000912.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1SBQ X-ray 2.20 A/B 1-164 [» ]
    1U3F X-ray 2.50 A/B 1-164 [» ]
    1U3G X-ray 2.50 A 1-164 [» ]
    ProteinModelPortali P75430.
    SMRi P75430. Positions 1-164.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272634.MPN348.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB96136 ; AAB96136 ; MPN_348 .
    GeneIDi 876747.
    KEGGi mpn:MPN348.
    PATRICi 20022056. VBIMycPne110_0375.

    Phylogenomic databases

    eggNOGi COG0212.
    OMAi KANHRIG.
    OrthoDBi EOG6RVG1P.

    Enzyme and pathway databases

    BioCyci MPNE272634:GJ6Z-365-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P75430.

    Family and domain databases

    Gene3Di 3.40.50.10420. 1 hit.
    InterProi IPR002698. FTHF_cligase.
    IPR024185. FTHF_cligase-like.
    [Graphical view ]
    PANTHERi PTHR23407:SF1. PTHR23407:SF1. 1 hit.
    Pfami PF01812. 5-FTHF_cyc-lig. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006806. FTHF_cligase. 1 hit.
    TIGRFAMsi TIGR02727. MTHFS_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
      Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
      Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29342 / M129.
    2. "Crystal structure of methenyltetrahydrofolate synthetase from Mycoplasma pneumoniae (GI: 13508087) at 2.2 A resolution."
      Chen S., Shin D.-H., Pufan R., Kim R., Kim S.H.
      Proteins 56:839-843(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS), SUBUNIT.
    3. "Structural and functional characterization of a 5,10-methenyltetrahydrofolate synthetase from Mycoplasma pneumoniae (GI: 13508087)."
      Chen S., Yakunin A.F., Proudfoot M., Kim R., Kim S.H.
      Proteins 61:433-443(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEX WITH SUBSTRATE; ADP AND MAGNESIUM, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, SUBUNIT.

    Entry informationi

    Entry nameiMTHFS_MYCPN
    AccessioniPrimary (citable) accession number: P75430
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Mycoplasma pneumoniae
      Mycoplasma pneumoniae (strain M129): entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3