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Protein

5-formyltetrahydrofolate cyclo-ligase

Gene

MPN_348

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in folate metabolism. Catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-FTHF) to yield 5,10-methenyltetrahydrofolate.1 Publication

Catalytic activityi

ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate.1 Publication

Cofactori

Mg2+1 Publication, Mn2+1 Publication, Ca2+1 Publication, Zn2+1 Publication, Fe2+1 Publication, Co2+1 Publication, Cu2+1 PublicationNote: Magnesium. It can also use divalent cations such as manganese, calcium, zinc, iron, cobalt and copper.1 Publication

Kineticsi

Kcat is 0.94 sec(-1) for cyclo-ligase activity with 5-FTHF (at pH 6 and 37 degrees Celsius). Kcat is 0.99 sec(-1) for cyclo-ligase activity with ATP (at pH 6 and 37 degrees Celsius).

  1. KM=165 µM for 5-FTHF (at pH 6 and 37 degrees Celsius)1 Publication
  2. KM=166 µM for ATP (at pH 6 and 37 degrees Celsius)1 Publication
  1. Vmax=2.70 µmol/min/mg enzyme toward 5-FTHF (at pH 6 and 37 degrees Celsius)1 Publication
  2. Vmax=2.84 µmol/min/mg enzyme toward ATP (at pH 6 and 37 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50Substrate1 Publication1
Binding sitei55Substrate1 Publication1
Metal bindingi124Magnesium1 Publication1
Binding sitei125ATP1
Binding sitei153ATP1
Metal bindingi154Magnesium1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi3 – 7ATP5
Nucleotide bindingi115 – 123ATPCurated9

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.3.2. 3534.

Names & Taxonomyi

Protein namesi
Recommended name:
5-formyltetrahydrofolate cyclo-ligase (EC:6.3.3.2)
Alternative name(s):
5,10-methenyl-tetrahydrofolate synthetase
Short name:
MTHFS
Short name:
Methenyl-THF synthetase
Gene namesi
Ordered Locus Names:MPN_348
ORF Names:H91_orf164, MP488
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000808 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002002881 – 1645-formyltetrahydrofolate cyclo-ligaseAdd BLAST164

Interactioni

Subunit structurei

Monomer or homodimer.2 Publications

Structurei

Secondary structure

1164
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 16Combined sources14
Helixi19 – 37Combined sources19
Beta strandi45 – 47Combined sources3
Helixi60 – 68Combined sources9
Beta strandi72 – 78Combined sources7
Beta strandi80 – 82Combined sources3
Beta strandi84 – 87Combined sources4
Helixi96 – 98Combined sources3
Beta strandi101 – 105Combined sources5
Beta strandi107 – 109Combined sources3
Beta strandi114 – 116Combined sources3
Beta strandi118 – 120Combined sources3
Helixi122 – 126Combined sources5
Helixi127 – 129Combined sources3
Beta strandi136 – 140Combined sources5
Helixi142 – 144Combined sources3
Beta strandi158 – 162Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SBQX-ray2.20A/B1-164[»]
1U3FX-ray2.50A/B1-164[»]
1U3GX-ray2.50A1-164[»]
ProteinModelPortaliP75430.
SMRiP75430.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP75430.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK01934.
OMAiCVFTPLV.

Family and domain databases

Gene3Di3.40.50.10420. 1 hit.
InterProiIPR002698. FTHF_cligase.
IPR024185. FTHF_cligase-like.
[Graphical view]
PANTHERiPTHR23407:SF1. PTHR23407:SF1. 1 hit.
PfamiPF01812. 5-FTHF_cyc-lig. 1 hit.
[Graphical view]
PIRSFiPIRSF006806. FTHF_cligase. 1 hit.
TIGRFAMsiTIGR02727. MTHFS_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

P75430-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKNALRKQI LQKRMALSTI EKSHLDQKIN QKLVAFLTPK PCIKTIALYE
60 70 80 90 100
PIKNEVTFVD FFFEFLKINQ IRAVYPKVIS DTEIIFIDQE TNTFEPNQID
110 120 130 140 150
CFLIPLVGFN KDNYRLGFGK GYYDRYLMQL TRQQPKIGIA YSFQKGDFLA
160
DPWDVQLDLI INDE
Length:164
Mass (Da):19,265
Last modified:February 1, 1997 - v1
Checksum:iD44365FDE62A9DDF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB96136.1.
PIRiS73814.
RefSeqiNP_110036.1. NC_000912.1.
WP_010874704.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB96136; AAB96136; MPN_348.
GeneIDi876747.
KEGGimpn:MPN348.
PATRICi20022056. VBIMycPne110_0375.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB96136.1.
PIRiS73814.
RefSeqiNP_110036.1. NC_000912.1.
WP_010874704.1. NC_000912.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SBQX-ray2.20A/B1-164[»]
1U3FX-ray2.50A/B1-164[»]
1U3GX-ray2.50A1-164[»]
ProteinModelPortaliP75430.
SMRiP75430.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB96136; AAB96136; MPN_348.
GeneIDi876747.
KEGGimpn:MPN348.
PATRICi20022056. VBIMycPne110_0375.

Phylogenomic databases

KOiK01934.
OMAiCVFTPLV.

Enzyme and pathway databases

BRENDAi6.3.3.2. 3534.

Miscellaneous databases

EvolutionaryTraceiP75430.

Family and domain databases

Gene3Di3.40.50.10420. 1 hit.
InterProiIPR002698. FTHF_cligase.
IPR024185. FTHF_cligase-like.
[Graphical view]
PANTHERiPTHR23407:SF1. PTHR23407:SF1. 1 hit.
PfamiPF01812. 5-FTHF_cyc-lig. 1 hit.
[Graphical view]
PIRSFiPIRSF006806. FTHF_cligase. 1 hit.
TIGRFAMsiTIGR02727. MTHFS_bact. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMTHFS_MYCPN
AccessioniPrimary (citable) accession number: P75430
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.