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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei572 – 5721ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMPNE272634:GJ6Z-405-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:MPN_384
ORF Names:MP453
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000808: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 793793Leucine--tRNA ligasePRO_0000152050Add
BLAST

Proteomic databases

PaxDbiP75398.

Interactioni

Protein-protein interaction databases

STRINGi272634.MPN384.

Structurei

3D structure databases

ProteinModelPortaliP75398.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi39 – 5012"HIGH" regionAdd
BLAST
Motifi569 – 5735"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P75398-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYNHNLLEEK WLQLWKTKQV NRFFDDKTKP KYYILDMFPY PSAAGLHLGH
60 70 80 90 100
VRAYTITDVL SRYHKAKGFN VIHPIGFDAF GLPAEQYAIA SNKHPGDWTD
110 120 130 140 150
KNITNFIEQL TAFGFDYDYQ LSLKTTDPRY YQYTQWIFGQ LFEAGLAEVK
160 170 180 190 200
EIDVNWCAEL GTVLANEEVL IDSNGNAVSE RGEFPVTKRK MKQWVLKITA
210 220 230 240 250
FAESLLNGLA ELDWHQSIKE MQTNWIGKSE GVEVTFDIEN SKETITIFTT
260 270 280 290 300
KVQTIFGVTF LALSNSHPLV KEVAKTNPKI AQFLQKQAQK TTTVKQPETL
310 320 330 340 350
HDGVDLKLKA INPATNTAIP LYVANYVVEG YGTDAVMGVP AHNENDNFFA
360 370 380 390 400
RKQKLPIITV IDKQERLQHS GQFSGLNSQT ANTQITQMLV ERQKAKKTTV
410 420 430 440 450
YKLRDWIFSR QRYWGEPFPI LFDENNQPHL VKELPVTLPA LANYQPDGST
460 470 480 490 500
NPPLWRNQEW AKVKQGNQTF TRETSTMPQW AGSCWYYLGY LMLINNENFW
510 520 530 540 550
PIDSREAKDL FERYLPVDLY VGGAEHAVLH LLYARFWHQF LYQKGIVTTK
560 570 580 590 600
EPFKKLINQG MVLGPDGKKM SKSKGNTINP TPLIDSHGAD ALRLYLMFMG
610 620 630 640 650
PISAALTWLD DGLNGMRRWL DRVHNFFHKE NIIKETVDQE TVYGYNLFLK
660 670 680 690 700
RSFEHLEKQE LNLVISQMMI FLNLLYKTKQ LTLAYAKGFL TVLSFFAPYL
710 720 730 740 750
AEELNAKLGM EPFIVHAQLP NVDNSVLETD KVKIILSVNG RFKGTKEFAK
760 770 780 790
GVDEQTVLKA FKTDPEFQAL FDQPLARVVF VPNRIINVLL KSE
Length:793
Mass (Da):91,084
Last modified:February 1, 1997 - v1
Checksum:iDAD90195932B62D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB96101.1.
PIRiS73779.
RefSeqiNP_110072.1. NC_000912.1.
WP_010874740.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB96101; AAB96101; MPN_384.
GeneIDi877105.
KEGGimpn:MPN384.
PATRICi20022144. VBIMycPne110_0415.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB96101.1.
PIRiS73779.
RefSeqiNP_110072.1. NC_000912.1.
WP_010874740.1. NC_000912.1.

3D structure databases

ProteinModelPortaliP75398.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272634.MPN384.

Proteomic databases

PaxDbiP75398.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB96101; AAB96101; MPN_384.
GeneIDi877105.
KEGGimpn:MPN384.
PATRICi20022144. VBIMycPne110_0415.

Phylogenomic databases

eggNOGiCOG0495.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciMPNE272634:GJ6Z-405-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
    Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
    Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29342 / M129.

Entry informationi

Entry nameiSYL_MYCPN
AccessioniPrimary (citable) accession number: P75398
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: February 4, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.