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P75398 (SYL_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:MPN_384
ORF Names:MP453
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length793 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 793793Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152050

Regions

Motif39 – 5012"HIGH" region HAMAP-Rule MF_00049
Motif569 – 5735"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5721ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P75398 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: DAD90195932B62D9

FASTA79391,084
        10         20         30         40         50         60 
MYNHNLLEEK WLQLWKTKQV NRFFDDKTKP KYYILDMFPY PSAAGLHLGH VRAYTITDVL 

        70         80         90        100        110        120 
SRYHKAKGFN VIHPIGFDAF GLPAEQYAIA SNKHPGDWTD KNITNFIEQL TAFGFDYDYQ 

       130        140        150        160        170        180 
LSLKTTDPRY YQYTQWIFGQ LFEAGLAEVK EIDVNWCAEL GTVLANEEVL IDSNGNAVSE 

       190        200        210        220        230        240 
RGEFPVTKRK MKQWVLKITA FAESLLNGLA ELDWHQSIKE MQTNWIGKSE GVEVTFDIEN 

       250        260        270        280        290        300 
SKETITIFTT KVQTIFGVTF LALSNSHPLV KEVAKTNPKI AQFLQKQAQK TTTVKQPETL 

       310        320        330        340        350        360 
HDGVDLKLKA INPATNTAIP LYVANYVVEG YGTDAVMGVP AHNENDNFFA RKQKLPIITV 

       370        380        390        400        410        420 
IDKQERLQHS GQFSGLNSQT ANTQITQMLV ERQKAKKTTV YKLRDWIFSR QRYWGEPFPI 

       430        440        450        460        470        480 
LFDENNQPHL VKELPVTLPA LANYQPDGST NPPLWRNQEW AKVKQGNQTF TRETSTMPQW 

       490        500        510        520        530        540 
AGSCWYYLGY LMLINNENFW PIDSREAKDL FERYLPVDLY VGGAEHAVLH LLYARFWHQF 

       550        560        570        580        590        600 
LYQKGIVTTK EPFKKLINQG MVLGPDGKKM SKSKGNTINP TPLIDSHGAD ALRLYLMFMG 

       610        620        630        640        650        660 
PISAALTWLD DGLNGMRRWL DRVHNFFHKE NIIKETVDQE TVYGYNLFLK RSFEHLEKQE 

       670        680        690        700        710        720 
LNLVISQMMI FLNLLYKTKQ LTLAYAKGFL TVLSFFAPYL AEELNAKLGM EPFIVHAQLP 

       730        740        750        760        770        780 
NVDNSVLETD KVKIILSVNG RFKGTKEFAK GVDEQTVLKA FKTDPEFQAL FDQPLARVVF 

       790 
VPNRIINVLL KSE 

« Hide

References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB96101.1.
PIRS73779.
RefSeqNP_110072.1. NC_000912.1.

3D structure databases

ProteinModelPortalP75398.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272634.MPN384.

Proteomic databases

PaxDbP75398.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB96101; AAB96101; MPN_384.
GeneID877105.
KEGGmpn:MPN384.
PATRIC20022144. VBIMycPne110_0415.

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycMPNE272634:GJ6Z-405-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_MYCPN
AccessionPrimary (citable) accession number: P75398
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries