P75382 (SYP_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	MPN_402
ORF Names:	MP436

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma pneumoniae

Protein attributes

Sequence length	483 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP-Rule MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP-Rule MF_01571
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01571
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP-Rule MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 483	483	Proline--tRNA ligase HAMAP-Rule MF_01571		PRO_0000139335

Sequences

	Sequence		Length	Mass (Da)
	P75382 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: D5F4205B325951A6	FASTA	483	55,455
	10 20 30 40 50 60 MANKDQNLTL WYDQLLSKAQ LVSYGDVKGT NCFLPNSWNL WLQIQRLYNN ATALIKLKDK 70 80 90 100 110 120 VILKQFIPIE PLPYTVEQVQ LPTLSFYSEY QKEKRHVEGF NPELFLIEQI GTKKLHDPLV 130 140 150 160 170 180 LRPTSEIAFC NLWKKQSFSY QNLPVIYNQW TCVFRAEKNT RPFLRNSEFY WQETHGLFSD 190 200 210 220 230 240 GVNSESAAIA FWKLYQDIIV NQLCIPAFVG LKSPNERFAG AQNTWTVESI MPDGQALQCA 250 260 270 280 290 300 TSHDLGQTFT KPFGLTFQNQ ANQQAIPYSF SCGISTRILG ALLLTHSDDF GLVLPWKVAP 310 320 330 340 350 360 IQVKLYLFDK KGDTKTVELA QKVQTLLEQL AIRFQFIKVE NQLGKQLGQG EVNGIPFQLI 370 380 390 400 410 420 VDNPQTVNIF NRLTRVKTAY SFEQLASRFV ELVQQYHQAM YDKAKAVVQQ KVVQATTLKQ 430 440 450 460 470 480 IEQAFNDKKA VLCAVRLTDT LEQQLKERYQ VTVRCCLEQL QKPQICPFSG ESAQDYVLIA RAY

References

[1]

"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB96084.1.
PIR	S73762.
RefSeq	NP_110090.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P75382.
ModBase	Search...
Protein-protein interaction databases
IntAct	P75382. 1 interaction.
STRING	272634.MPN402.
Proteomic databases
PaxDb	P75382.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB96084; AAB96084; MPN_402.
GeneID	877119.
KEGG	mpn:MPN402.
PATRIC	20022194. VBIMycPne110_0435.
Phylogenomic databases
eggNOG	COG0442.
KO	K01881.
OMA	LQCATSH.
ProtClustDB	PRK08661.
Enzyme and pathway databases
BioCyc	MPNE272634:GJ6Z-430-MONOMER.
Family and domain databases
Gene3D	3.30.110.30. 1 hit. 3.40.50.800. 1 hit.
HAMAP	MF_01571. Pro_tRNA_synth_type3.
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-ligase_IIa. IPR004499. Pro-tRNA-ligase_IIa_arc-type. IPR016061. Pro-tRNA_ligase_II_C. IPR017449. Pro-tRNA_synth_II. [Graphical view]
PANTHER	PTHR11451:SF6. PTHR11451:SF6. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. proS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_MYCPN

Accession

Primary (citable) accession number: P75382

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 29, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program