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P75382 (SYP_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proline--tRNA ligase

EC=6.1.1.15
Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS
Gene names
Name:proS
Ordered Locus Names:MPN_402
ORF Names:MP436
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length483 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP-Rule MF_01571

Catalytic activity

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP-Rule MF_01571

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01571

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01571.

Domain

Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP-Rule MF_01571

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprolyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

proline-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 483483Proline--tRNA ligase HAMAP-Rule MF_01571
PRO_0000139335

Sequences

Sequence LengthMass (Da)Tools
P75382 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: D5F4205B325951A6

FASTA48355,455
        10         20         30         40         50         60 
MANKDQNLTL WYDQLLSKAQ LVSYGDVKGT NCFLPNSWNL WLQIQRLYNN ATALIKLKDK 

        70         80         90        100        110        120 
VILKQFIPIE PLPYTVEQVQ LPTLSFYSEY QKEKRHVEGF NPELFLIEQI GTKKLHDPLV 

       130        140        150        160        170        180 
LRPTSEIAFC NLWKKQSFSY QNLPVIYNQW TCVFRAEKNT RPFLRNSEFY WQETHGLFSD 

       190        200        210        220        230        240 
GVNSESAAIA FWKLYQDIIV NQLCIPAFVG LKSPNERFAG AQNTWTVESI MPDGQALQCA 

       250        260        270        280        290        300 
TSHDLGQTFT KPFGLTFQNQ ANQQAIPYSF SCGISTRILG ALLLTHSDDF GLVLPWKVAP 

       310        320        330        340        350        360 
IQVKLYLFDK KGDTKTVELA QKVQTLLEQL AIRFQFIKVE NQLGKQLGQG EVNGIPFQLI 

       370        380        390        400        410        420 
VDNPQTVNIF NRLTRVKTAY SFEQLASRFV ELVQQYHQAM YDKAKAVVQQ KVVQATTLKQ 

       430        440        450        460        470        480 
IEQAFNDKKA VLCAVRLTDT LEQQLKERYQ VTVRCCLEQL QKPQICPFSG ESAQDYVLIA 


RAY 

« Hide

References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB96084.1.
PIRS73762.
RefSeqNP_110090.1. NC_000912.1.

3D structure databases

ProteinModelPortalP75382.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP75382. 1 interaction.
STRING272634.MPN402.

Proteomic databases

PaxDbP75382.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB96084; AAB96084; MPN_402.
GeneID877119.
KEGGmpn:MPN402.
PATRIC20022194. VBIMycPne110_0435.

Phylogenomic databases

eggNOGCOG0442.
KOK01881.
OMALQCATSH.
OrthoDBEOG6G7R2R.
ProtClustDBPRK08661.

Enzyme and pathway databases

BioCycMPNE272634:GJ6Z-430-MONOMER.

Family and domain databases

Gene3D3.30.110.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPMF_01571. Pro_tRNA_synth_type3.
InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004499. Pro-tRNA-ligase_IIa_arc-type.
IPR016061. Pro-tRNA_ligase_II_C.
IPR017449. Pro-tRNA_synth_II.
[Graphical view]
PANTHERPTHR11451:SF6. PTHR11451:SF6. 1 hit.
PfamPF03129. HGTP_anticodon. 1 hit.
PF09180. ProRS-C_1. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSPR01046. TRNASYNTHPRO.
SUPFAMSSF52954. SSF52954. 1 hit.
SSF64586. SSF64586. 1 hit.
TIGRFAMsTIGR00408. proS_fam_I. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYP_MYCPN
AccessionPrimary (citable) accession number: P75382
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: February 19, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries