ID   Y427_MYCPN              Reviewed;         290 AA.
AC   P75360;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   27-MAR-2024, entry version 109.
DE   RecName: Full=Putative phosphatase MPN_427;
DE            EC=3.1.3.-;
GN   OrderedLocusNames=MPN_427; ORFNames=A05_orf290, MP414;
OS   Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1)
OS   (Mycoplasmoides pneumoniae).
OC   Bacteria; Mycoplasmatota; Mycoplasmoidales; Mycoplasmoidaceae;
OC   Mycoplasmoides.
OX   NCBI_TaxID=272634;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29342 / M129 / Subtype 1;
RX   PubMed=8948633; DOI=10.1093/nar/24.22.4420;
RA   Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.;
RT   "Complete sequence analysis of the genome of the bacterium Mycoplasma
RT   pneumoniae.";
RL   Nucleic Acids Res. 24:4420-4449(1996).
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the HAD-like hydrolase superfamily. Cof family.
CC       {ECO:0000305}.
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DR   EMBL; U00089; AAB96062.1; -; Genomic_DNA.
DR   PIR; S73740; S73740.
DR   RefSeq; NP_110115.1; NC_000912.1.
DR   RefSeq; WP_010874783.1; NZ_OU342337.1.
DR   AlphaFoldDB; P75360; -.
DR   SMR; P75360; -.
DR   STRING; 272634.MPN_427; -.
DR   EnsemblBacteria; AAB96062; AAB96062; MPN_427.
DR   KEGG; mpn:MPN_427; -.
DR   PATRIC; fig|272634.6.peg.462; -.
DR   HOGENOM; CLU_044146_0_1_14; -.
DR   OrthoDB; 9810101at2; -.
DR   BioCyc; MPNE272634:G1GJ3-691-MONOMER; -.
DR   Proteomes; UP000000808; Chromosome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016791; F:phosphatase activity; IEA:UniProt.
DR   Gene3D; 3.30.1240.10; -; 1.
DR   Gene3D; 3.40.50.1000; HAD superfamily/HAD-like; 1.
DR   InterPro; IPR000150; Cof.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR006379; HAD-SF_hydro_IIB.
DR   InterPro; IPR023214; HAD_sf.
DR   NCBIfam; TIGR00099; Cof-subfamily; 1.
DR   NCBIfam; TIGR01484; HAD-SF-IIB; 1.
DR   PANTHER; PTHR10000:SF8; PHOSPHOGLYCOLATE PHOSPHATASE 1; 1.
DR   PANTHER; PTHR10000; PHOSPHOSERINE PHOSPHATASE; 1.
DR   Pfam; PF08282; Hydrolase_3; 1.
DR   SUPFAM; SSF56784; HAD-like; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Reference proteome.
FT   CHAIN           1..290
FT                   /note="Putative phosphatase MPN_427"
FT                   /id="PRO_0000054441"
FT   ACT_SITE        16
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   BINDING         16
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         17
FT                   /ligand="phosphate"
FT                   /ligand_id="ChEBI:CHEBI:43474"
FT                   /evidence="ECO:0000250"
FT   BINDING         18
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         53..54
FT                   /ligand="phosphate"
FT                   /ligand_id="ChEBI:CHEBI:43474"
FT                   /evidence="ECO:0000250"
FT   BINDING         216
FT                   /ligand="phosphate"
FT                   /ligand_id="ChEBI:CHEBI:43474"
FT                   /evidence="ECO:0000250"
FT   BINDING         239
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         240
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         242
FT                   /ligand="phosphate"
FT                   /ligand_id="ChEBI:CHEBI:43474"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   290 AA;  33216 MW;  FCA0416EDEEE48CA CRC64;
     MTKTSKASGL SWFFCDLDGT LLRYQNNQHL IEPTTKRAVA QLVESGANFV VATGRKPSDV
     RNIYKELGIE QASPYLIANN GAVVWDLKRN SYLNKQTLSL SDFDLIDHIN QTLNQLNHEY
     GCILYGLNDQ VYFYHIHAPD SQAFKQYFAF YEGEFVQNQY LEIDGLKTEY NLVKAIWFFK
     EVHQQKAVIA QHFTNQERLV ITSAHSFELV PLNVSKGHAI NLIKQQVKIT DNQIMVLGDS
     YNDLPMFQHG VVKVTNHLAP DNLKQLATRV YELPASLFVG QALNDYFKFD
//