P75305 (AZOR_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
FMN-dependent NADH-azoreductase
EC=1.7.-.-

Alternative name(s):
Azo-dye reductase
FMN-dependent NADH-azo compound oxidoreductase

Gene names

Name:	azoR
Ordered Locus Names:	MPN_479
ORF Names:	MP362

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma pneumoniae

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity By similarity. HAMAP-Rule MF_01216
Cofactor	Binds 1 FMN per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the azoreductase type 1 family.

Ontologies

Keywords
Ligand	FMN Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	FMN binding Inferred from electronic annotation. Source: HAMAP FMN reductase activity Inferred from electronic annotation. Source: HAMAP electron carrier activity Inferred from electronic annotation. Source: HAMAP oxidoreductase activity, acting on NAD(P)H, NAD(P) as acceptor Inferred from electronic annotation. Source: HAMAP oxidoreductase activity, acting on other nitrogenous compounds as donors Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MPN_040	P75074	1	EBI-2260147,EBI-2260151

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 197	197	FMN-dependent NADH-azoreductase HAMAP-Rule MF_01216		PRO_0000166345

Sequences

	Sequence		Length	Mass (Da)
	P75305 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: B566B394F7936D31	FASTA	197	21,550
	10 20 30 40 50 60 MKKVIIIDAS VSPSGSYTHL LLERFLATFQ AKNKDVELST WNLNELPVGQ ISYNTQNAGT 70 80 90 100 110 120 FFSVENSDKY IDALKAAHGV IILAPMTNFN YPATLKNFID HVFVANKTFK DKYVTKGASS 130 140 150 160 170 180 GMLGNLKVVV LGSQGAPLGW YPWGDHVNSL RGLFGFAGVA SFASVIIDGT KLLYKDKSKS 190 EVVDMFAKQV DAIANEF

References

[1]

"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB96010.1.
PIR	S73688.
RefSeq	NP_110167.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P75305.
ModBase	Search...
Protein-protein interaction databases
IntAct	P75305. 1 interaction.
STRING	272634.MPN479.
Proteomic databases
PaxDb	P75305.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB96010; AAB96010; MPN_479.
GeneID	876744.
KEGG	mpn:MPN479.
PATRIC	20022382. VBIMycPne110_0518.
Phylogenomic databases
eggNOG	COG1182.
KO	K01118.
OMA	ESDFWIN.
Enzyme and pathway databases
BioCyc	MPNE272634:GJ6Z-520-MONOMER.
Family and domain databases
HAMAP	MF_01216. Azoreductase_type1.
InterPro	IPR003680. Flavodoxin_fold. IPR023048. NADH-azoreductase_FMN-depdnt. [Graphical view]
Pfam	PF02525. Flavodoxin_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AZOR_MYCPN

Accession

Primary (citable) accession number: P75305

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program