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P75302

- PBDGT_MYCPN

UniProt

P75302 - PBDGT_MYCPN

Protein

Processive diacylglycerol beta-glycosyltransferase

Gene

MPN_483

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Processive glycosyltransferase involved in the biosynthesis of both the non-bilayer-prone beta-monoglycosyldiacylglycerol and the bilayer-forming membrane lipid glucosyl-galactosyldiacylglycerol and digalactosyl-diacylglycerol. These components contribute to regulate the properties and stability of the membrane. Catalyzes sequentially the transfers of glucosyl or galactosyl residues from UDP-Glc or UDP-Gal to diacylglycerol (DAG) acceptor to form the corresponding beta-glycosyl-DAG (3-O-(beta-D-glycopyranosyl)-1,2-diacyl-sn-glycerol). Then, only beta-galactosyl-DAG (3-O-(beta-D-galactopyranosyl)-1,2-diacyl-sn-glycerol) can act as acceptor to give the beta-glycosyl-beta-galactosyl-DAG product (3-O-(beta-D-glycopyranosyl-(1->6)-D-galactopyranosyl)-1,2-diacyl-sn-glycerol). It can also use alpha-Gal-beta-Gal-DAG, ceramide (Cer) and beta-Gal-Cer as sugar acceptors. The enzyme is supposed to be mainly a galactosyltransferase, with higher glycosyltransferase activity for the addition of the second glycosyl on beta-Gal-DAG as acceptor. The main glycolipid produced in vivo is beta-Glc-beta-Gal-DAG with a beta-1,6 linkage.1 Publication

    Catalytic activityi

    UDP-glucose + 1,2-diacyl-sn-glycerol = UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol.1 Publication
    UDP-galactose + 1,2-diacyl-sn-glycerol = UDP + 1,2-diacyl-3-O-(beta-D-galactopyranosyl)-sn-glycerol.1 Publication
    UDP-glucose + 1,2-diacyl-3-O-(beta-D-galactopyranosyl)-sn-glycerol = UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-galactopyranosyl)-sn-glycerol.1 Publication
    UDP-galactose + 1,2-diacyl-3-O-(beta-D-galactopyranosyl)-sn-glycerol = UDP + 1,2-diacyl-3-O-(beta-D-galactopyranosyl-(1->6)-O-beta-D-galactopyranosyl)-sn-glycerol.1 Publication

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by the negatively charged lipid phosphatidylglycerol (PG).1 Publication

    GO - Molecular functioni

    1. transferase activity, transferring glycosyl groups Source: UniProtKB-KW

    GO - Biological processi

    1. glycerol metabolic process Source: UniProtKB-KW
    2. membrane lipid biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glycerol metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciMPNE272634:GJ6Z-524-MONOMER.

    Protein family/group databases

    CAZyiGT2. Glycosyltransferase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Processive diacylglycerol beta-glycosyltransferase (EC:2.4.1.-)
    Alternative name(s):
    Beta-monoglycosyldiacylglycerol synthase
    Short name:
    Beta-MGS
    Short name:
    MGlyDAG synthase
    Diglycosyldiacylglycerol synthase
    Short name:
    Beta-DGS
    Short name:
    DGlyDAG synthase
    Glycosyl-beta-1,6-galactosyldiacylglycerol synthase
    UDP-galactose:1,2-diacylglycerol 3-beta-D-galactosyltransferase
    UDP-glucose:1,2-diacylglycerol 3-beta-D-glucosyltransferase
    Gene namesi
    Ordered Locus Names:MPN_483
    ORF Names:MP359, P01_orf341
    OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
    Taxonomic identifieri272634 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000808: Chromosome

    Subcellular locationi

    Cell membrane By similarity

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 341341Processive diacylglycerol beta-glycosyltransferasePRO_0000059246Add
    BLAST

    Proteomic databases

    PaxDbiP75302.

    Interactioni

    Protein-protein interaction databases

    IntActiP75302. 3 interactions.
    STRINGi272634.MPN483.

    Structurei

    3D structure databases

    ProteinModelPortaliP75302.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 2 family.Curated

    Phylogenomic databases

    eggNOGiCOG0463.
    OrthoDBiEOG6WQD5X.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR001173. Glyco_trans_2-like.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PfamiPF00535. Glycos_transf_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P75302-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNKLISILVP CYQSQPFLDR FFKSLLKQDW NGVKVIFFND NKPDPTYEIL    50
    KQFQQAHPQL AIEVHCGEKN VGVGGSRDQL INYVDTPYFY FVDPDDEFSD 100
    PNCFKAIVET IQGENFDIAV LNSIVYLQML KNDFLIKHIP LKNIFQGKVK 150
    LNPDNTVNHL HYIQNNDQYI WNIVINTAFF KALDLQFVNR FIEDIAVWFP 200
    IMFKAQKVLW IDVNGVNYYL RPNSASTQKN SIKLLSFIEA YERLYFHLKK 250
    VGKLADFIDP NNKIESRFWR RQAFIWFSFI NVSWMKAEFE QTKSVLQKLF 300
    DFMEANGIYD RVFTNKHHGI YLLWVNRLKH FKKLVQAQPH L 341
    Length:341
    Mass (Da):40,415
    Last modified:February 1, 1997 - v1
    Checksum:iC209F50D714CB3D0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB96007.1.
    PIRiS73685.
    RefSeqiNP_110171.1. NC_000912.1.

    Genome annotation databases

    EnsemblBacteriaiAAB96007; AAB96007; MPN_483.
    GeneIDi876759.
    KEGGimpn:MPN483.
    PATRICi20022390. VBIMycPne110_0522.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB96007.1 .
    PIRi S73685.
    RefSeqi NP_110171.1. NC_000912.1.

    3D structure databases

    ProteinModelPortali P75302.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P75302. 3 interactions.
    STRINGi 272634.MPN483.

    Protein family/group databases

    CAZyi GT2. Glycosyltransferase Family 2.

    Proteomic databases

    PaxDbi P75302.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB96007 ; AAB96007 ; MPN_483 .
    GeneIDi 876759.
    KEGGi mpn:MPN483.
    PATRICi 20022390. VBIMycPne110_0522.

    Phylogenomic databases

    eggNOGi COG0463.
    OrthoDBi EOG6WQD5X.

    Enzyme and pathway databases

    BioCyci MPNE272634:GJ6Z-524-MONOMER.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR001173. Glyco_trans_2-like.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    Pfami PF00535. Glycos_transf_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
      Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
      Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29342 / M129.
    2. "A processive lipid glycosyltransferase in the small human pathogen Mycoplasma pneumoniae: involvement in host immune response."
      Klement M.L., Ojemyr L., Tagscherer K.E., Widmalm G., Wieslander A.
      Mol. Microbiol. 65:1444-1457(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBSTRATE SPECIFICITY.
      Strain: ATCC 29342 / M129.

    Entry informationi

    Entry nameiPBDGT_MYCPN
    AccessioniPrimary (citable) accession number: P75302
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The local lipid environment around the enzyme affects both the extent of head group elongation and total amounts of glycolipids produced.1 Publication
    Glycolipids such as beta-Gal-DAG, alpha-Gal-beta-Gal-DAG, beta-Glc-beta-Gal-DAG and beta-Gal-Cer are highly immunogenic and are reactive towards IgM antibodies. Glycolipids with a terminal beta-Gal are more reactive than the ones with a beta-Glc residue (PubMed:17697098).1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Mycoplasma pneumoniae
      Mycoplasma pneumoniae (strain M129): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3