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P75302 (PBDGT_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Processive diacylglycerol beta-glycosyltransferase

EC=2.4.1.-
Alternative name(s):
Beta-monoglycosyldiacylglycerol synthase
Short name=Beta-MGS
Short name=MGlyDAG synthase
Diglycosyldiacylglycerol synthase
Short name=Beta-DGS
Short name=DGlyDAG synthase
Glycosyl-beta-1,6-galactosyldiacylglycerol synthase
UDP-galactose:1,2-diacylglycerol 3-beta-D-galactosyltransferase
UDP-glucose:1,2-diacylglycerol 3-beta-D-glucosyltransferase
Gene names
Ordered Locus Names:MPN_483
ORF Names:MP359, P01_orf341
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Processive glycosyltransferase involved in the biosynthesis of both the non-bilayer-prone beta-monoglycosyldiacylglycerol and the bilayer-forming membrane lipid glucosyl-galactosyldiacylglycerol and digalactosyl-diacylglycerol. These components contribute to regulate the properties and stability of the membrane. Catalyzes sequentially the transfers of glucosyl or galactosyl residues from UDP-Glc or UDP-Gal to diacylglycerol (DAG) acceptor to form the corresponding beta-glycosyl-DAG (3-O-(beta-D-glycopyranosyl)-1,2-diacyl-sn-glycerol). Then, only beta-galactosyl-DAG (3-O-(beta-D-galactopyranosyl)-1,2-diacyl-sn-glycerol) can act as acceptor to give the beta-glycosyl-beta-galactosyl-DAG product (3-O-(beta-D-glycopyranosyl-(1->6)-D-galactopyranosyl)-1,2-diacyl-sn-glycerol). It can also use alpha-Gal-beta-Gal-DAG, ceramide (Cer) and beta-Gal-Cer as sugar acceptors. The enzyme is supposed to be mainly a galactosyltransferase, with higher glycosyltransferase activity for the addition of the second glycosyl on beta-Gal-DAG as acceptor. The main glycolipid produced in vivo is beta-Glc-beta-Gal-DAG with a beta-1,6 linkage. Ref.2

Catalytic activity

UDP-glucose + 1,2-diacyl-sn-glycerol = UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol. Ref.2

UDP-galactose + 1,2-diacyl-sn-glycerol = UDP + 1,2-diacyl-3-O-(beta-D-galactopyranosyl)-sn-glycerol. Ref.2

UDP-glucose + 1,2-diacyl-3-O-(beta-D-galactopyranosyl)-sn-glycerol = UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-galactopyranosyl)-sn-glycerol. Ref.2

UDP-galactose + 1,2-diacyl-3-O-(beta-D-galactopyranosyl)-sn-glycerol = UDP + 1,2-diacyl-3-O-(beta-D-galactopyranosyl-(1->6)-O-beta-D-galactopyranosyl)-sn-glycerol. Ref.2

Cofactor

Magnesium By similarity.

Enzyme regulation

Activated by the negatively charged lipid phosphatidylglycerol (PG). Ref.2

Subcellular location

Cell membrane By similarity.

Miscellaneous

The local lipid environment around the enzyme affects both the extent of head group elongation and total amounts of glycolipids produced (Ref.2).

Glycolipids such as beta-Gal-DAG, alpha-Gal-beta-Gal-DAG, beta-Glc-beta-Gal-DAG and beta-Gal-Cer are highly immunogenic and are reactive towards IgM antibodies. Glycolipids with a terminal beta-Gal are more reactive than the ones with a beta-Glc residue (Ref.2).

Sequence similarities

Belongs to the glycosyltransferase 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 341341Processive diacylglycerol beta-glycosyltransferase
PRO_0000059246

Sequences

Sequence LengthMass (Da)Tools
P75302 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: C209F50D714CB3D0

FASTA34140,415
        10         20         30         40         50         60 
MNKLISILVP CYQSQPFLDR FFKSLLKQDW NGVKVIFFND NKPDPTYEIL KQFQQAHPQL 

        70         80         90        100        110        120 
AIEVHCGEKN VGVGGSRDQL INYVDTPYFY FVDPDDEFSD PNCFKAIVET IQGENFDIAV 

       130        140        150        160        170        180 
LNSIVYLQML KNDFLIKHIP LKNIFQGKVK LNPDNTVNHL HYIQNNDQYI WNIVINTAFF 

       190        200        210        220        230        240 
KALDLQFVNR FIEDIAVWFP IMFKAQKVLW IDVNGVNYYL RPNSASTQKN SIKLLSFIEA 

       250        260        270        280        290        300 
YERLYFHLKK VGKLADFIDP NNKIESRFWR RQAFIWFSFI NVSWMKAEFE QTKSVLQKLF 

       310        320        330        340 
DFMEANGIYD RVFTNKHHGI YLLWVNRLKH FKKLVQAQPH L 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.
[2]"A processive lipid glycosyltransferase in the small human pathogen Mycoplasma pneumoniae: involvement in host immune response."
Klement M.L., Ojemyr L., Tagscherer K.E., Widmalm G., Wieslander A.
Mol. Microbiol. 65:1444-1457(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, SUBSTRATE SPECIFICITY.
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB96007.1.
PIRS73685.
RefSeqNP_110171.1. NC_000912.1.

3D structure databases

ProteinModelPortalP75302.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP75302. 3 interactions.
STRING272634.MPN483.

Protein family/group databases

CAZyGT2. Glycosyltransferase Family 2.

Proteomic databases

PaxDbP75302.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB96007; AAB96007; MPN_483.
GeneID876759.
KEGGmpn:MPN483.
PATRIC20022390. VBIMycPne110_0522.

Phylogenomic databases

eggNOGCOG0463.
OMADIFIANC.
OrthoDBEOG6WQD5X.
ProtClustDBCLSK335097.

Enzyme and pathway databases

BioCycMPNE272634:GJ6Z-524-MONOMER.

Family and domain databases

InterProIPR001173. Glyco_trans_2-like.
[Graphical view]
PfamPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePBDGT_MYCPN
AccessionPrimary (citable) accession number: P75302
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names