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P75258

- SYI_MYCPN

UniProt

P75258 - SYI_MYCPN

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Protein
Isoleucine--tRNA ligase
Gene
ileS, MPN_520, MP322
Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei549 – 5491Aminoacyl-adenylate By similarity
Binding sitei593 – 5931ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMPNE272634:GJ6Z-561-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:MPN_520
ORF Names:MP322
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000808: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 861861Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098424Add
BLAST

Proteomic databases

PaxDbiP75258.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi272634.MPN520.

Structurei

3D structure databases

ProteinModelPortaliP75258.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi590 – 5945"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P75258-1 [UniParc]FASTAAdd to Basket

« Hide

MNLKKTLLMP QTAFEMQGKL TTKEQQFQAF WQSKRIYQKL HRQNKDKPQK    50
ILHDGPPYAN GNIHVGHALN KILKDFVLRS WNLQGFGTVF IPGWDCHGLP 100
IEHAVSKKDP QHYASLSLSE KRDLCKQFAL SQIAIQKAQF QRLGLLNDFS 150
KYYKTIDESF QQNELDLFLQ AVKKDLIFQA LKPTYWSPVS RTSLAEAEIE 200
YKEVKTIGLY LTFTVVQSAV LNSGTKLLVW TTTPWTLPTN QAIAVHPQFE 250
YLLFTYNNEQ YVVLASLFES LKTKFGWTDA IQVQTISGSQ LQNTTYKHCL 300
YDKVNPVLLG NHVLCNEGTG LVHTSPAYGL DDFYLCKQNK LNEALVSLDE 350
KGVFNDTLND PVLTGLFYLK ANDVIIERLK QHHNFVFSES FLHREPHDWR 400
SKTPVIYRAS KQLFIKTKSI QSKLKRQIKR VKFVNNKNKE RLQEMLLQRA 450
EWCISRQRVW GLPIPLIYAD NQPLLDVTTI KYTIQQLKKY GIDSWFEKDI 500
NFFLNPKKIQ PGVEYKKETD TLEVWFDSGS TYNVLISNKL NFPADLYLEG 550
SDQYRGWFNS SASCGIIQTD QLPFKSLISH GFTLDEHGNK MSKSLGNVVD 600
PLKLCDQYGA DILRLWVTNV DWQVDNRIGD NIIKQIVEQY RRIRNSLLRF 650
ILGNLNHFNF GEMKDYRFAL EDKIVIHKTN ALVQELHQWL KQYNFLNCLK 700
AINKFVLWLS GWYFEIIKDT LYCDAKTNPN RVAKQAVLNY IFTQLIGFLN 750
IFIPHTAEDA WQNYLLPKKP VSVNLFAGPA MFKVANVKGL DRLHESFSAI 800
KDRAYAAIEQ ARQNGVITKN NQVVLTLGVD STSAIDPTSC KTFSSLAKRK 850
PGKYNEWPQR N 861
Length:861
Mass (Da):99,527
Last modified:February 1, 1997 - v1
Checksum:i6A521066C0EE235D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00089 Genomic DNA. Translation: AAB95970.1.
PIRiS73648.
RefSeqiNP_110208.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB95970; AAB95970; MPN_520.
GeneIDi876906.
KEGGimpn:MPN520.
PATRICi20022500. VBIMycPne110_0577.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00089 Genomic DNA. Translation: AAB95970.1 .
PIRi S73648.
RefSeqi NP_110208.1. NC_000912.1.

3D structure databases

ProteinModelPortali P75258.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272634.MPN520.

Proteomic databases

PaxDbi P75258.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB95970 ; AAB95970 ; MPN_520 .
GeneIDi 876906.
KEGGi mpn:MPN520.
PATRICi 20022500. VBIMycPne110_0577.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci MPNE272634:GJ6Z-561-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
    Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
    Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29342 / M129.

Entry informationi

Entry nameiSYI_MYCPN
AccessioniPrimary (citable) accession number: P75258
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 14, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi