P75258 (SYI_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	MPN_520
ORF Names:	MP322

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma pneumoniae

Protein attributes

Sequence length	861 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002
Subunit structure	Monomer By similarity. HAMAP-Rule MF_02002
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_02002.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 861	861	Isoleucine--tRNA ligase HAMAP-Rule MF_02002		PRO_0000098424
Regions
	Motif	57 – 67	11	"HIGH" region HAMAP-Rule MF_02002
	Motif	590 – 594	5	"KMSKS" region HAMAP-Rule MF_02002
Sites
	Binding site	549	1	Aminoacyl-adenylate By similarity
	Binding site	593	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P75258 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 6A521066C0EE235D	FASTA	861	99,527
	10 20 30 40 50 60 MNLKKTLLMP QTAFEMQGKL TTKEQQFQAF WQSKRIYQKL HRQNKDKPQK ILHDGPPYAN 70 80 90 100 110 120 GNIHVGHALN KILKDFVLRS WNLQGFGTVF IPGWDCHGLP IEHAVSKKDP QHYASLSLSE 130 140 150 160 170 180 KRDLCKQFAL SQIAIQKAQF QRLGLLNDFS KYYKTIDESF QQNELDLFLQ AVKKDLIFQA 190 200 210 220 230 240 LKPTYWSPVS RTSLAEAEIE YKEVKTIGLY LTFTVVQSAV LNSGTKLLVW TTTPWTLPTN 250 260 270 280 290 300 QAIAVHPQFE YLLFTYNNEQ YVVLASLFES LKTKFGWTDA IQVQTISGSQ LQNTTYKHCL 310 320 330 340 350 360 YDKVNPVLLG NHVLCNEGTG LVHTSPAYGL DDFYLCKQNK LNEALVSLDE KGVFNDTLND 370 380 390 400 410 420 PVLTGLFYLK ANDVIIERLK QHHNFVFSES FLHREPHDWR SKTPVIYRAS KQLFIKTKSI 430 440 450 460 470 480 QSKLKRQIKR VKFVNNKNKE RLQEMLLQRA EWCISRQRVW GLPIPLIYAD NQPLLDVTTI 490 500 510 520 530 540 KYTIQQLKKY GIDSWFEKDI NFFLNPKKIQ PGVEYKKETD TLEVWFDSGS TYNVLISNKL 550 560 570 580 590 600 NFPADLYLEG SDQYRGWFNS SASCGIIQTD QLPFKSLISH GFTLDEHGNK MSKSLGNVVD 610 620 630 640 650 660 PLKLCDQYGA DILRLWVTNV DWQVDNRIGD NIIKQIVEQY RRIRNSLLRF ILGNLNHFNF 670 680 690 700 710 720 GEMKDYRFAL EDKIVIHKTN ALVQELHQWL KQYNFLNCLK AINKFVLWLS GWYFEIIKDT 730 740 750 760 770 780 LYCDAKTNPN RVAKQAVLNY IFTQLIGFLN IFIPHTAEDA WQNYLLPKKP VSVNLFAGPA 790 800 810 820 830 840 MFKVANVKGL DRLHESFSAI KDRAYAAIEQ ARQNGVITKN NQVVLTLGVD STSAIDPTSC 850 860 KTFSSLAKRK PGKYNEWPQR N

References

[1]

"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB95970.1.
PIR	S73648.
RefSeq	NP_110208.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P75258.
ModBase	Search...
Protein-protein interaction databases
STRING	272634.MPN520.
Proteomic databases
PaxDb	P75258.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB95970; AAB95970; MPN_520.
GeneID	876906.
KEGG	mpn:MPN520.
PATRIC	20022500. VBIMycPne110_0577.
Phylogenomic databases
eggNOG	COG0060.
KO	K01870.
OMA	KQVLTHG.
ProtClustDB	PRK13804.
Enzyme and pathway databases
BioCyc	MPNE272634:GJ6Z-561-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
HAMAP	MF_02002. Ile_tRNA_synth_type1.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR023585. Ile-tRNA-ligase_type1. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
PANTHER	PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_MYCPN

Accession

Primary (citable) accession number: P75258

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program