Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P75256 (TRMB_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:MPN_522
ORF Names:G12_orf210V, MP320
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length210 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 210210tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171359

Regions

Region188 – 1914Substrate binding By similarity

Sites

Active site1121 By similarity
Binding site361S-adenosyl-L-methionine By similarity
Binding site611S-adenosyl-L-methionine By similarity
Binding site901S-adenosyl-L-methionine By similarity
Binding site1121S-adenosyl-L-methionine By similarity
Binding site1161Substrate By similarity
Binding site1481Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P75256 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 2A41D406B3C8B83C

FASTA21024,542
        10         20         30         40         50         60 
MRLRKVKDAL VRVQDSPYFS NPEQLSTIES ESLFVEIGCG KSWFLSQQAQ QNPKCFFVGI 

        70         80         90        100        110        120 
EREPTIVLKA INKVNRLEVK PRNLLITCLD ANQLTEYLKP QSISKIFINF PDPWPKKRHT 

       130        140        150        160        170        180 
LRRLTAPHFL KQFHQLLKKQ GLIEFKTDND QLFEFTLEVL QKTVAHFKII EQTTDLHNSP 

       190        200        210 
LTSTNIMTEY EQRFVSLGVK IKKLTLEKLN 

« Hide

References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB95968.1.
PIRS73646.
RefSeqNP_110210.1. NC_000912.1.

3D structure databases

ProteinModelPortalP75256.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272634.MPN522.

Proteomic databases

PaxDbP75256.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB95968; AAB95968; MPN_522.
GeneID876965.
KEGGmpn:MPN522.
PATRIC20022504. VBIMycPne110_0579.

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OMAKTDDDEL.
OrthoDBEOG6K6VBC.
ProtClustDBCLSK335295.

Enzyme and pathway databases

BioCycMPNE272634:GJ6Z-563-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_MYCPN
AccessionPrimary (citable) accession number: P75256
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: April 16, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names