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Protein

Acetate kinase

Gene

ackA

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.UniRule annotation

Catalytic activityi

ATP + acetate = ADP + acetyl phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Mg2+. Can also accept Mn2+.UniRule annotation

Pathwayi: acetyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA from acetate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetate kinase (ackA)
  2. Phosphate acetyltransferase (pta)
This subpathway is part of the pathway acetyl-CoA biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from acetate, the pathway acetyl-CoA biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10MagnesiumUniRule annotation1
Binding sitei17ATPUniRule annotation1
Binding sitei89SubstrateUniRule annotation1
Active sitei146Proton donor/acceptorUniRule annotation1
Sitei178Transition state stabilizerUniRule annotation1
Sitei237Transition state stabilizerUniRule annotation1
Metal bindingi376MagnesiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi204 – 208ATPUniRule annotation5
Nucleotide bindingi278 – 280ATPUniRule annotation3
Nucleotide bindingi323 – 327ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-603.
BRENDAi2.7.2.1. 3534.
UniPathwayiUPA00340; UER00458.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
Alternative name(s):
AcetokinaseUniRule annotation
Gene namesi
Name:ackAUniRule annotation
Ordered Locus Names:MPN_533
ORF Names:MP309
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000808 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: AgBase

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001075881 – 390Acetate kinaseAdd BLAST390

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiP75245. 1 interactor.

Structurei

3D structure databases

ProteinModelPortaliP75245.
SMRiP75245.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acetokinase family.UniRule annotation

Phylogenomic databases

KOiK00925.
OMAiWKALTGT.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase. 1 hit.
InterProiView protein in InterPro
IPR004372. Ac/propionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiView protein in Pfam
PF00871. Acetate_kinase. 1 hit.
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiView protein in PROSITE
PS01075. ACETATE_KINASE_1. 1 hit.
PS01076. ACETATE_KINASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P75245-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDNKILVVN AGSSSIKFQL FDYHKKVLAK ALCERIFVDG FFKLEFNEQK
60 70 80 90 100
VEEKVAFPDH HAAVTHFLNT LKKHKIIQEL SDIILVGHRV VQGANYFKDS
110 120 130 140 150
VIVDAEALAK IKEFIKLAPL HNKPEADVIE IFFKEVPSAK NVAVFDTTFH
160 170 180 190 200
TTIPQENYLY AVPRSWEQKH LVRRYGFHGT SYKFINNYLE KHLNKQNLNL
210 220 230 240 250
IVCHLGNGAS VCAIKNGKSF NTSMGFTPLE GLIMGTRSGD LDPAIIGYVA
260 270 280 290 300
EQENMSASDV VNALNKKSGM LALTGASDMR DVFAKPQENA VAIKMYVNRV
310 320 330 340 350
ADYIAKYLNQ LEGNIDGLVF TGGIGENASD CVELFINAVK SLGFATDLKL
360 370 380 390
FVKYGDSCVV STPQSKYKIY RVRTNEELMI VEDSIRLTQK
Length:390
Mass (Da):43,700
Last modified:February 1, 1997 - v1
Checksum:i6E24F005205AE5E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB95957.1.
PIRiS73635.
RefSeqiNP_110222.1. NC_000912.1.
WP_010874890.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB95957; AAB95957; MPN_533.
GeneIDi877249.
KEGGimpn:MPN533.
PATRICifig|272634.6.peg.594.

Similar proteinsi

Entry informationi

Entry nameiACKA_MYCPN
AccessioniPrimary (citable) accession number: P75245
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 7, 2017
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families