P75233 (RNC_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease 3
EC=3.1.26.3

Alternative name(s):
Ribonuclease III
Short name=RNase III

Gene names

Name:	rnc
Ordered Locus Names:	MPN_545
ORF Names:	MP297

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma pneumoniae

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104
Cofactor	Mg2+ By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 RNase III domain.

Ontologies

Keywords
Biological process	mRNA processing rRNA processing tRNA processing
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC rRNA catabolic process Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease III activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	Ribonuclease 3 HAMAP-Rule MF_00104		PRO_0000180411
Regions
	Domain	18 – 141	124	RNase III
Sites
	Active site	63	1	Potential
	Active site	130	1	By similarity
	Metal binding	59	1	Magnesium By similarity
	Metal binding	127	1	Magnesium By similarity
	Metal binding	130	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P75233 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 47C2BC66F7450D8C	FASTA	282	32,653
	10 20 30 40 50 60 MKNKKDKTQK PKVIDEKFVA FFKSLNIEPQ NWQFYEDAFV HSSYVNENED ARASYDRLEF 70 80 90 100 110 120 LGDALIDFIV AKKLFELYPN YNEGMLTRTK IEIVKGENLN RIGKELNFGN FIKLGKGMPY 130 140 150 160 170 180 TETLFGDVLE ALVAAIYEDL GIEKANQFVE EHIFKKTYSE ILKYNFFSLF QEQKLPEPRV 190 200 210 220 230 240 RVSLTSNNLV LSIIELNGDI IWSQAVPNSK HYDDKSVLEH NAMSAFTQFL KSGKGINFFS 250 260 270 280 DIKNKLDSQK PMRALTVRPK KINWKARKPK LKALKNKVKA DS

References

[1]

"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB95945.1.
PIR	S73623.
RefSeq	NP_110234.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P75233.
ModBase	Search...
Protein-protein interaction databases
STRING	272634.MPN545.
Proteomic databases
PaxDb	P75233.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB95945; AAB95945; MPN_545.
GeneID	876735.
KEGG	mpn:MPN545.
PATRIC	20022567. VBIMycPne110_0607.
Phylogenomic databases
eggNOG	COG0571.
KO	K03685.
OMA	VLEHNAM.
ProtClustDB	CLSK335305.
Enzyme and pathway databases
BioCyc	MPNE272634:GJ6Z-590-MONOMER.
Family and domain databases
Gene3D	1.10.1520.10. 1 hit.
HAMAP	MF_00104. RNase_III.
InterPro	IPR011907. RNase_III. IPR000999. RNase_III_dom. [Graphical view]
PANTHER	PTHR11207. PTHR11207. 1 hit.
Pfam	PF00636. Ribonuclease_3. 1 hit. [Graphical view]
SMART	SM00535. RIBOc. 1 hit. [Graphical view]
SUPFAM	SSF69065. RNase_III. 1 hit.
TIGRFAMs	TIGR02191. RNaseIII. 1 hit.
PROSITE	PS00517. RNASE_3_1. 1 hit. PS50142. RNASE_3_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNC_MYCPN

Accession

Primary (citable) accession number: P75233

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program