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Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

Catalytic activityi

ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster.UniRule annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei269ATPUniRule annotation1
Binding sitei287ATP; via amide nitrogenUniRule annotation1
Binding sitei296ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi185 – 186ATPUniRule annotation2
Nucleotide bindingi210 – 211ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Transferase, tRNA-binding
Biological processThiamine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
Thiamine biosynthesis protein ThiIUniRule annotation
tRNA 4-thiouridine synthaseUniRule annotation
Gene namesi
Name:thiIUniRule annotation
Ordered Locus Names:MPN_550
ORF Names:MP292
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000808 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001548511 – 387Probable tRNA sulfurtransferaseAdd BLAST387

Structurei

3D structure databases

ProteinModelPortaliP75228.
SMRiP75228.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini67 – 167THUMPUniRule annotationAdd BLAST101

Sequence similaritiesi

Belongs to the ThiI family.UniRule annotation

Phylogenomic databases

KOiK03151.
OMAiKLFPEIM.

Family and domain databases

CDDicd01712. ThiI. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI. 1 hit.
InterProiView protein in InterPro
IPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP_dom.
IPR003720. tRNA_STrfase.
PfamiView protein in Pfam
PF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
SMARTiView protein in SMART
SM00981. THUMP. 1 hit.
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiView protein in PROSITE
PS51165. THUMP. 1 hit.

Sequencei

Sequence statusi: Complete.

P75228-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLHNEPNTI LICRYGELVL KGKNRLQFVK QLKKNVKQAF KKLSITNPVD
60 70 80 90 100
YQFDMLVVGE VISTQRSLLK NLFTRLPGLS VCLFALQIPH DEAQLLALLQ
110 120 130 140 150
QVVQSHPSFK IEVRRRDKLF ACNSSAFKKY LALQLWEKYQ LKGKLVDPAI
160 170 180 190 200
TVHVEVTKEH FLIISESFNG IGGLPVFTSG TALALLSGGI DSPVAASLVL
210 220 230 240 250
QRGFNVDFIT FINEPGHNAA TIGKIQRLAN LVSLNQTLCT GRLFVFDFTD
260 270 280 290 300
LQKELSHISL EGYRIVLMRR CFYKIASLFK YDCLITGEAL GQVASQTIDN
310 320 330 340 350
LKVIQAVVPN TFVIRPLIGL SKDKIIEWAK ALGTFETSIE HHMDTCTVFA
360 370 380
PKKPTTKAKL AIVEKLESEL LFVRELIEAG VKKLQND
Length:387
Mass (Da):43,341
Last modified:February 1, 1997 - v1
Checksum:iE418669E7A8F64DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB95940.1.
PIRiS73618.
RefSeqiNP_110239.1. NC_000912.1.
WP_010874907.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB95940; AAB95940; MPN_550.
GeneIDi877210.
KEGGimpn:MPN550.
PATRICifig|272634.6.peg.612.

Similar proteinsi

Entry informationi

Entry nameiTHII_MYCPN
AccessioniPrimary (citable) accession number: P75228
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: August 30, 2017
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families