Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P75228 (THII_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable tRNA sulfurtransferase

EC=2.8.1.4
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferase
Thiamine biosynthesis protein ThiI
tRNA 4-thiouridine synthase
Gene names
Name:thiI
Ordered Locus Names:MPN_550
ORF Names:MP292
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length387 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS By similarity. HAMAP-Rule MF_00021

Catalytic activity

L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide. HAMAP-Rule MF_00021

[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP. HAMAP-Rule MF_00021

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis. HAMAP-Rule MF_00021

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00021.

Sequence similarities

Belongs to the ThiI family.

Contains 1 THUMP domain.

Ontologies

Keywords
   Biological processThiamine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
RNA-binding
tRNA-binding
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA thio-modification

Inferred from electronic annotation. Source: UniProtKB-HAMAP

thiamine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

thiamine diphosphate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

sulfurtransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself1EBI-2260824,EBI-2260824

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 387387Probable tRNA sulfurtransferase HAMAP-Rule MF_00021
PRO_0000154851

Regions

Domain67 – 167101THUMP
Nucleotide binding185 – 1862ATP By similarity
Nucleotide binding210 – 2112ATP By similarity

Sites

Binding site2691ATP By similarity
Binding site2871ATP; via amide nitrogen By similarity
Binding site2961ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P75228 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: E418669E7A8F64DF

FASTA38743,341
        10         20         30         40         50         60 
MGLHNEPNTI LICRYGELVL KGKNRLQFVK QLKKNVKQAF KKLSITNPVD YQFDMLVVGE 

        70         80         90        100        110        120 
VISTQRSLLK NLFTRLPGLS VCLFALQIPH DEAQLLALLQ QVVQSHPSFK IEVRRRDKLF 

       130        140        150        160        170        180 
ACNSSAFKKY LALQLWEKYQ LKGKLVDPAI TVHVEVTKEH FLIISESFNG IGGLPVFTSG 

       190        200        210        220        230        240 
TALALLSGGI DSPVAASLVL QRGFNVDFIT FINEPGHNAA TIGKIQRLAN LVSLNQTLCT 

       250        260        270        280        290        300 
GRLFVFDFTD LQKELSHISL EGYRIVLMRR CFYKIASLFK YDCLITGEAL GQVASQTIDN 

       310        320        330        340        350        360 
LKVIQAVVPN TFVIRPLIGL SKDKIIEWAK ALGTFETSIE HHMDTCTVFA PKKPTTKAKL 

       370        380 
AIVEKLESEL LFVRELIEAG VKKLQND 

« Hide

References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB95940.1.
PIRS73618.
RefSeqNP_110239.1. NC_000912.1.

3D structure databases

ProteinModelPortalP75228.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272634.MPN550.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB95940; AAB95940; MPN_550.
GeneID877210.
KEGGmpn:MPN550.
PATRIC20022577. VBIMycPne110_0612.

Phylogenomic databases

eggNOGCOG0301.
KOK03151.
OMAKLTPYVG.
OrthoDBEOG6TBHGR.
ProtClustDBPRK01565.

Enzyme and pathway databases

BioCycMPNE272634:GJ6Z-595-MONOMER.
UniPathwayUPA00060.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00021. ThiI.
InterProIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_C_dom.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsTIGR00342. TIGR00342. 1 hit.
PROSITEPS51165. THUMP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTHII_MYCPN
AccessionPrimary (citable) accession number: P75228
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: February 19, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names