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P75228

- THII_MYCPN

UniProt

P75228 - THII_MYCPN

Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotation

    Catalytic activityi

    L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotation
    [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei269 – 2691ATPUniRule annotation
    Binding sitei287 – 2871ATP; via amide nitrogenUniRule annotation
    Binding sitei296 – 2961ATPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi185 – 1862ATPUniRule annotation
    Nucleotide bindingi210 – 2112ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. sulfurtransferase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-HAMAP
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
    3. tRNA thio-modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Thiamine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

    Enzyme and pathway databases

    BioCyciMPNE272634:GJ6Z-595-MONOMER.
    UniPathwayiUPA00060.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
    Alternative name(s):
    Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
    Thiamine biosynthesis protein ThiIUniRule annotation
    tRNA 4-thiouridine synthaseUniRule annotation
    Gene namesi
    Name:thiIUniRule annotation
    Ordered Locus Names:MPN_550
    ORF Names:MP292
    OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
    Taxonomic identifieri272634 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000808: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 387387Probable tRNA sulfurtransferasePRO_0000154851Add
    BLAST

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself1EBI-2260824,EBI-2260824

    Protein-protein interaction databases

    STRINGi272634.MPN550.

    Structurei

    3D structure databases

    ProteinModelPortaliP75228.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini67 – 167101THUMPUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ThiI family.UniRule annotation
    Contains 1 THUMP domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0301.
    KOiK03151.
    OMAiDCCVLFS.
    OrthoDBiEOG6TBHGR.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    HAMAPiMF_00021. ThiI.
    InterProiIPR014729. Rossmann-like_a/b/a_fold.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view]
    PfamiPF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view]
    SMARTiSM00981. THUMP. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
    PROSITEiPS51165. THUMP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P75228-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLHNEPNTI LICRYGELVL KGKNRLQFVK QLKKNVKQAF KKLSITNPVD    50
    YQFDMLVVGE VISTQRSLLK NLFTRLPGLS VCLFALQIPH DEAQLLALLQ 100
    QVVQSHPSFK IEVRRRDKLF ACNSSAFKKY LALQLWEKYQ LKGKLVDPAI 150
    TVHVEVTKEH FLIISESFNG IGGLPVFTSG TALALLSGGI DSPVAASLVL 200
    QRGFNVDFIT FINEPGHNAA TIGKIQRLAN LVSLNQTLCT GRLFVFDFTD 250
    LQKELSHISL EGYRIVLMRR CFYKIASLFK YDCLITGEAL GQVASQTIDN 300
    LKVIQAVVPN TFVIRPLIGL SKDKIIEWAK ALGTFETSIE HHMDTCTVFA 350
    PKKPTTKAKL AIVEKLESEL LFVRELIEAG VKKLQND 387
    Length:387
    Mass (Da):43,341
    Last modified:February 1, 1997 - v1
    Checksum:iE418669E7A8F64DF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB95940.1.
    PIRiS73618.
    RefSeqiNP_110239.1. NC_000912.1.

    Genome annotation databases

    EnsemblBacteriaiAAB95940; AAB95940; MPN_550.
    GeneIDi877210.
    KEGGimpn:MPN550.
    PATRICi20022577. VBIMycPne110_0612.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB95940.1 .
    PIRi S73618.
    RefSeqi NP_110239.1. NC_000912.1.

    3D structure databases

    ProteinModelPortali P75228.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272634.MPN550.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB95940 ; AAB95940 ; MPN_550 .
    GeneIDi 877210.
    KEGGi mpn:MPN550.
    PATRICi 20022577. VBIMycPne110_0612.

    Phylogenomic databases

    eggNOGi COG0301.
    KOi K03151.
    OMAi DCCVLFS.
    OrthoDBi EOG6TBHGR.

    Enzyme and pathway databases

    UniPathwayi UPA00060 .
    BioCyci MPNE272634:GJ6Z-595-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    HAMAPi MF_00021. ThiI.
    InterProi IPR014729. Rossmann-like_a/b/a_fold.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view ]
    Pfami PF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view ]
    SMARTi SM00981. THUMP. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00342. TIGR00342. 1 hit.
    PROSITEi PS51165. THUMP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
      Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
      Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29342 / M129.

    Entry informationi

    Entry nameiTHII_MYCPN
    AccessioniPrimary (citable) accession number: P75228
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Mycoplasma pneumoniae
      Mycoplasma pneumoniae (strain M129): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3