P75228 (THII_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable tRNA sulfurtransferase
EC=2.8.1.4

Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferase
Thiamine biosynthesis protein ThiI
tRNA 4-thiouridine synthase

Gene names

Name:	thiI
Ordered Locus Names:	MPN_550
ORF Names:	MP292

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma pneumoniae

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS By similarity. HAMAP-Rule MF_00021
Catalytic activity	L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide. HAMAP-Rule MF_00021 [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP. HAMAP-Rule MF_00021
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis. HAMAP-Rule MF_00021
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ThiI family. Contains 1 THUMP domain.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA thio-modification Inferred from electronic annotation. Source: HAMAP thiamine biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamine diphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP sulfurtransferase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-2260824,EBI-2260824

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	Probable tRNA sulfurtransferase HAMAP-Rule MF_00021		PRO_0000154851
Regions
	Domain	67 – 167	101	THUMP
	Nucleotide binding	185 – 186	2	ATP By similarity
	Nucleotide binding	210 – 211	2	ATP By similarity
Sites
	Binding site	269	1	ATP By similarity
	Binding site	287	1	ATP; via amide nitrogen By similarity
	Binding site	296	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P75228 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: E418669E7A8F64DF	FASTA	387	43,341
	10 20 30 40 50 60 MGLHNEPNTI LICRYGELVL KGKNRLQFVK QLKKNVKQAF KKLSITNPVD YQFDMLVVGE 70 80 90 100 110 120 VISTQRSLLK NLFTRLPGLS VCLFALQIPH DEAQLLALLQ QVVQSHPSFK IEVRRRDKLF 130 140 150 160 170 180 ACNSSAFKKY LALQLWEKYQ LKGKLVDPAI TVHVEVTKEH FLIISESFNG IGGLPVFTSG 190 200 210 220 230 240 TALALLSGGI DSPVAASLVL QRGFNVDFIT FINEPGHNAA TIGKIQRLAN LVSLNQTLCT 250 260 270 280 290 300 GRLFVFDFTD LQKELSHISL EGYRIVLMRR CFYKIASLFK YDCLITGEAL GQVASQTIDN 310 320 330 340 350 360 LKVIQAVVPN TFVIRPLIGL SKDKIIEWAK ALGTFETSIE HHMDTCTVFA PKKPTTKAKL 370 380 AIVEKLESEL LFVRELIEAG VKKLQND

References

[1]

"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB95940.1.
PIR	S73618.
RefSeq	NP_110239.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P75228.
ModBase	Search...
Protein-protein interaction databases
STRING	272634.MPN550.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAB95940; AAB95940; MPN_550.
GeneID	877210.
KEGG	mpn:MPN550.
PATRIC	20022577. VBIMycPne110_0612.
Phylogenomic databases
eggNOG	COG0301.
KO	K03151.
OMA	KLTPYVG.
ProtClustDB	PRK01565.
Enzyme and pathway databases
BioCyc	MPNE272634:GJ6Z-595-MONOMER.
UniPathway	UPA00060.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00021. ThiI.
InterPro	IPR014729. Rossmann-like_a/b/a_fold. IPR003720. ThiI. IPR020536. ThiI_C_dom. IPR004114. THUMP. [Graphical view]
Pfam	PF02568. ThiI. 1 hit. PF02926. THUMP. 1 hit. [Graphical view]
SMART	SM00981. THUMP. 1 hit. [Graphical view]
TIGRFAMs	TIGR00342. TIGR00342. 1 hit.
PROSITE	PS51165. THUMP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THII_MYCPN

Accession

Primary (citable) accession number: P75228

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program