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Protein

Enolase

Gene

eno

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gapA)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei169SubstrateUniRule annotation1
Binding sitei178SubstrateUniRule annotation1
Active sitei219Proton donorUniRule annotation1
Metal bindingi256MagnesiumUniRule annotation1
Metal bindingi310MagnesiumUniRule annotation1
Binding sitei310SubstrateUniRule annotation1
Metal bindingi337MagnesiumUniRule annotation1
Binding sitei337SubstrateUniRule annotation1
Active sitei362Proton acceptorUniRule annotation1
Binding sitei362Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei413SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-551.
UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotation
Ordered Locus Names:MPN_606
ORF Names:MP236
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000808 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation

  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

  • cell surface Source: UniProtKB-SubCell
  • cytosol Source: AgBase
  • extracellular region Source: UniProtKB-SubCell
  • phosphopyruvate hydratase complex Source: InterPro

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001339301 – 456EnolaseAdd BLAST456

Interactioni

Protein-protein interaction databases

IntActiP75189. 6 interactors.

Structurei

3D structure databases

ProteinModelPortaliP75189.
SMRiP75189.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni389 – 392Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

KOiK01689.
OMAiEFMIIPV.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P75189-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAQTGTDLF KIADLFAYQV FDSRGFPTVA CVVKLASGHT GEAMVPSGAS
60 70 80 90 100
TGEKEAIELR DGDPKAYFGK GVSQAVQNVN QTIAPKLIGL NATDQAAIDA
110 120 130 140 150
LMIQLDGTPN KAKLGANAIL AVSLAVAKAA ASAQKTSLFK YLANQVMGLN
160 170 180 190 200
KTEFILTVPM LNVINGGAHA DNNIDFQEFM IMPLGANSMH QALKMASETF
210 220 230 240 250
HALQKLLKQR GLNTNKGDEG GFAPNLKLAE EALDLMVEAI KAAGYQPGSD
260 270 280 290 300
IAIALDVAAS EFYDDTTKRY VFKKGIKAKI LDEKEWSLTT AQMIAYLKKL
310 320 330 340 350
TEQYPIISIE DGLSEHDWEG METLTKTLGQ HIQIVGDDLY CTNPAIAEKG
360 370 380 390 400
VAHKATNSIL IKLNQIGTLT ETIKAINIAK DANWSQVISH RSGETEDTTI
410 420 430 440 450
ADLAVAACTG QIKTGSMSRS ERIAKYNRLL QIELELGNNA KYLGWNTFKN

IKPQKA
Length:456
Mass (Da):49,228
Last modified:February 1, 1997 - v1
Checksum:i5B4B5442ADDC0E86
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti309 – 311IED → HRS (PubMed:8604303).Curated3
Sequence conflicti327 – 332TLGQHI → HWSTH in AAC43651 (PubMed:8604303).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB95884.1.
U43738 Genomic DNA. Translation: AAC43651.1.
PIRiS73562.
RefSeqiNP_110295.1. NC_000912.1.
WP_010874963.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB95884; AAB95884; MPN_606.
GeneIDi876984.
KEGGimpn:MPN606.
PATRICifig|272634.6.peg.669.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB95884.1.
U43738 Genomic DNA. Translation: AAC43651.1.
PIRiS73562.
RefSeqiNP_110295.1. NC_000912.1.
WP_010874963.1. NC_000912.1.

3D structure databases

ProteinModelPortaliP75189.
SMRiP75189.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP75189. 6 interactors.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB95884; AAB95884; MPN_606.
GeneIDi876984.
KEGGimpn:MPN606.
PATRICifig|272634.6.peg.669.

Phylogenomic databases

KOiK01689.
OMAiEFMIIPV.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.
BioCyciMetaCyc:MONOMER-551.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENO_MYCPN
AccessioniPrimary (citable) accession number: P75189
Secondary accession number(s): Q50324
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 7, 2017
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.