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P75132 (TRMD_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(1)-)-methyltransferase

EC=2.1.1.228
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene names
Name:trmD
Ordered Locus Names:MPN_659
ORF Names:MP183
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length231 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP-Rule MF_00605

Catalytic activity

S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine37 in tRNA. HAMAP-Rule MF_00605

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00605

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00605.

Sequence similarities

Belongs to the RNA methyltransferase TrmD family.

Sequence caution

The sequence AAB95831.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine(37)-N(1))-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 231231tRNA (guanine-N(1)-)-methyltransferase HAMAP-Rule MF_00605
PRO_0000060417

Regions

Region129 – 1346S-adenosyl-L-methionine binding By similarity

Sites

Binding site1101S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
P75132 [UniParc].

Last modified December 6, 2002. Version 2.
Checksum: 3DE57E826D842C5A

FASTA23126,340
        10         20         30         40         50         60 
MKITVLTLFE QVVWPYLNAS IMAQAQKAKL VEFEVINWRQ YCKDKHQTVD DMAYGGGGGM 

        70         80         90        100        110        120 
VLKAEPILKA LKACRTPQSK VVLLSPEGQQ FSQPMAQALT QTEHLILICG HYEGFDYRLY 

       130        140        150        160        170        180 
KHVDQIISLG DFVLSGGELV ALSVIDATVR LIKGVINDQS LIHESFNNYL LDFPAYTRPY 

       190        200        210        220        230 
DLDGDKVPEI LLSGDHKKIE AYRKEQQLLR TAQYRPDLYK QYLAKKDEKN K 

« Hide

References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB95831.1. Different initiation.
RefSeqNP_110348.1. NC_000912.1.

3D structure databases

ProteinModelPortalP75132.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272634.MPN659.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB95831; AAB95831; MPN_659.
GeneID877016.
KEGGmpn:MPN659.
PATRIC20022803. VBIMycPne110_0724.

Phylogenomic databases

eggNOGCOG0336.
KOK00554.
OMALYVDFEI.
OrthoDBEOG6J48RZ.

Enzyme and pathway databases

BioCycMPNE272634:GJ6Z-705-MONOMER.

Family and domain databases

Gene3D1.10.1270.20. 1 hit.
3.40.1280.10. 1 hit.
HAMAPMF_00605. TrmD.
InterProIPR029028. Alpha/beta_knot_MTases.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
IPR029026. tRNA_m1G_MTases_N.
IPR016009. tRNA_MeTrfase_TRMD/TRM10.
[Graphical view]
PfamPF01746. tRNA_m1G_MT. 1 hit.
[Graphical view]
PIRSFPIRSF000386. tRNA_mtase. 1 hit.
SUPFAMSSF75217. SSF75217. 1 hit.
TIGRFAMsTIGR00088. trmD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMD_MYCPN
AccessionPrimary (citable) accession number: P75132
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 6, 2002
Last modified: June 11, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names