P75114 (SYE_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	MPN_678
ORF Names:	MP164

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129)

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000119608
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022_B
	Motif	252 – 256	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	255	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P75114 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: FD7B7F4742B09A50	FASTA	484	55,621
	10 20 30 40 50 60 MEKIRTRYAP SPTGYLHVGG ARTAIFNFLL AKHFNGEFII RIEDTDTERN VEGGIESQLE 70 80 90 100 110 120 NLRWLGIIPD ESIYNPGNYG PYIQSQKLAT YKKLAYELVG KGLAYRCFCT KEKLEHERQL 130 140 150 160 170 180 ALEHHQTPKY LGTCRNLHSK HIQTNLDNQV PFTIRLKINQ DAEFAWNDQV RGKITIPGNS 190 200 210 220 230 240 LTDIVLLKAN GIATYNFAVV IDDHDMEITD VLRGAEHISN TAYQLAINQA LGYQRIPRFG 250 260 270 280 290 300 HLSVIVDKSG KKLSKRDTKT IQFIEQFKQE GYLPEAVVNF LALLGWNSDF NREFFTINQL 310 320 330 340 350 360 IESFTVNRVV GAPAFFDIKK LQWINAHYIK ELSDNAYFNF IDNYLTIDFD YLKNKRKEVS 370 380 390 400 410 420 LLFKNQLAFG IEINQLIKET FAPKLGVQHL SVKHRELFKE LQSALQQLSE QLQALPDWTK 430 440 450 460 470 480 DNVKSTLTQI GEQFNLKGKK LFMPLRLIFT NKEHGPDLAG IMVLHGKTQV LALLQEFIHA TNLF

References

[1]	"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB95812.1.
PIR	S73490.
RefSeq	NP_110367.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P75114.
ModBase	Search...
Protein-protein interaction databases
IntAct	P75114. 2 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	877376.
GenomeReviews	Gene locus MPN_678 in contig U00089_GR.
KEGG	mpn:MPN678.
PATRIC	20022845. VBIMycPne110_0745.
Phylogenomic databases
HOGENOM	HBG628189.
OMA	ESIIQFV.
PhylomeDB	P75114.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	MPNE272634:MPN678-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_MYCPN

Accession

Primary (citable) accession number: P75114

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program