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P75107 (SYS_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine--tRNA ligase

EC=6.1.1.11
Alternative name(s):
Seryl-tRNA synthetase
Short name=SerRS
Seryl-tRNA(Ser/Sec) synthetase
Gene names
Name:serS
Ordered Locus Names:MPN_005
ORF Names:MP149
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length420 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) By similarity. HAMAP-Rule MF_00176

Catalytic activity

ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser). HAMAP-Rule MF_00176

ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec). HAMAP-Rule MF_00176

Pathway

Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. HAMAP-Rule MF_00176

Subunit structure

Homodimer. The tRNA molecule binds across the dimer By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00176.

Domain

Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding By similarity. HAMAP-Rule MF_00176

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processselenocysteine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

selenocysteinyl-tRNA(Sec) biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

seryl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

serine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 420420Serine--tRNA ligase HAMAP-Rule MF_00176
PRO_0000122083

Regions

Nucleotide binding256 – 2583ATP By similarity
Nucleotide binding343 – 3464ATP By similarity
Region225 – 2273Serine binding By similarity

Sites

Binding site2791Serine By similarity
Binding site3791Serine By similarity

Sequences

Sequence LengthMass (Da)Tools
P75107 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 9BC071823D20998E

FASTA42047,924
        10         20         30         40         50         60 
MLDRNKLRNN LDFFKKKLVE RGVSESQFEA YVQADKAMRK LLHQIELANQ KQTLLAQQVA 

        70         80         90        100        110        120 
KKKGDPKLLK ESKELKQKLE QLNIAFKEAE TLSQELLLNL PNIADESVPV GRDETANLEL 

       130        140        150        160        170        180 
LKEGRKPVFD FTPLPHWELC ERLQLVAFDK ATKLTGARFV AYTDKAAKLL RAIASLMIDL 

       190        200        210        220        230        240 
NKNKYQEWNV PVIVNETSLT GTGQLPKFKD DVFKLENTRY YLSPTLEVQL ANLHANEIFT 

       250        260        270        280        290        300 
EGELPKYYTA TGVNFRQEAG SAGKQTKGTI RLHQFQKVEL VKFCKPSEAI HELEEMTRDA 

       310        320        330        340        350        360 
EQILLELKIP FRRLLLCSGD MGFSAQKTYD LEVWMAGCNE YREVSSCSSC GDFQARRAMI 

       370        380        390        400        410        420 
RYKDLTTGKN TYVATLNGTA LAIDRIFAAI LEHYQTKAGE VMIPQALLKY LDFDKITKPK 

« Hide

References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB95797.1.
PIRS73475.
RefSeqNP_109693.1. NC_000912.1.

3D structure databases

ProteinModelPortalP75107.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP75107. 1 interaction.
STRING272634.MPN005.

Proteomic databases

PaxDbP75107.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB95797; AAB95797; MPN_005.
GeneID877292.
KEGGmpn:MPN005.
PATRIC20021283. VBIMycPne110_0005.

Phylogenomic databases

eggNOGCOG0172.
KOK01875.
OMAMGTDVIK.
OrthoDBEOG61KBH9.

Enzyme and pathway databases

BioCycMPNE272634:GJ6Z-5-MONOMER.
UniPathwayUPA00906; UER00895.

Family and domain databases

Gene3D1.10.287.40. 1 hit.
HAMAPMF_00176. Ser_tRNA_synth_type1.
InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR002317. Ser-tRNA-ligase_type_1.
IPR015866. Ser-tRNA-synth_1_N.
IPR010978. tRNA-bd_arm.
[Graphical view]
PANTHERPTHR11778. PTHR11778. 1 hit.
PfamPF02403. Seryl_tRNA_N. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PIRSFPIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTSPR00981. TRNASYNTHSER.
SUPFAMSSF46589. SSF46589. 1 hit.
TIGRFAMsTIGR00414. serS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYS_MYCPN
AccessionPrimary (citable) accession number: P75107
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 14, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries