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Protein

Serine--tRNA ligase

Gene

serS

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).UniRule annotation

Catalytic activityi

ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser).UniRule annotation
ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec).UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei279 – 2791SerineUniRule annotation
Binding sitei379 – 3791SerineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi256 – 2583ATPUniRule annotation
Nucleotide bindingi343 – 3464ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. serine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. selenocysteine biosynthetic process Source: UniProtKB-HAMAP
  2. selenocysteinyl-tRNA(Sec) biosynthetic process Source: UniProtKB-UniPathway
  3. seryl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMPNE272634:GJ6Z-5-MONOMER.
UniPathwayiUPA00906; UER00895.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine--tRNA ligaseUniRule annotation (EC:6.1.1.11UniRule annotation)
Alternative name(s):
Seryl-tRNA synthetaseUniRule annotation
Short name:
SerRSUniRule annotation
Seryl-tRNA(Ser/Sec) synthetaseUniRule annotation
Gene namesi
Name:serSUniRule annotation
Ordered Locus Names:MPN_005
ORF Names:MP149
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000808: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 420420Serine--tRNA ligasePRO_0000122083Add
BLAST

Proteomic databases

PaxDbiP75107.

Interactioni

Subunit structurei

Homodimer. The tRNA molecule binds across the dimer.UniRule annotation

Protein-protein interaction databases

IntActiP75107. 1 interaction.
STRINGi272634.MPN005.

Structurei

3D structure databases

ProteinModelPortaliP75107.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni225 – 2273Serine bindingUniRule annotation

Domaini

Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0172.
KOiK01875.
OMAiYRIVRIC.
OrthoDBiEOG61KBH9.

Family and domain databases

Gene3Di1.10.287.40. 1 hit.
HAMAPiMF_00176. Ser_tRNA_synth_type1.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR002317. Ser-tRNA-ligase_type_1.
IPR015866. Ser-tRNA-synth_1_N.
IPR010978. tRNA-bd_arm.
[Graphical view]
PANTHERiPTHR11778. PTHR11778. 1 hit.
PfamiPF02403. Seryl_tRNA_N. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PIRSFiPIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTSiPR00981. TRNASYNTHSER.
SUPFAMiSSF46589. SSF46589. 1 hit.
TIGRFAMsiTIGR00414. serS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P75107-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLDRNKLRNN LDFFKKKLVE RGVSESQFEA YVQADKAMRK LLHQIELANQ
60 70 80 90 100
KQTLLAQQVA KKKGDPKLLK ESKELKQKLE QLNIAFKEAE TLSQELLLNL
110 120 130 140 150
PNIADESVPV GRDETANLEL LKEGRKPVFD FTPLPHWELC ERLQLVAFDK
160 170 180 190 200
ATKLTGARFV AYTDKAAKLL RAIASLMIDL NKNKYQEWNV PVIVNETSLT
210 220 230 240 250
GTGQLPKFKD DVFKLENTRY YLSPTLEVQL ANLHANEIFT EGELPKYYTA
260 270 280 290 300
TGVNFRQEAG SAGKQTKGTI RLHQFQKVEL VKFCKPSEAI HELEEMTRDA
310 320 330 340 350
EQILLELKIP FRRLLLCSGD MGFSAQKTYD LEVWMAGCNE YREVSSCSSC
360 370 380 390 400
GDFQARRAMI RYKDLTTGKN TYVATLNGTA LAIDRIFAAI LEHYQTKAGE
410 420
VMIPQALLKY LDFDKITKPK
Length:420
Mass (Da):47,924
Last modified:February 1, 1997 - v1
Checksum:i9BC071823D20998E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB95797.1.
PIRiS73475.
RefSeqiNP_109693.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB95797; AAB95797; MPN_005.
GeneIDi877292.
KEGGimpn:MPN005.
PATRICi20021283. VBIMycPne110_0005.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB95797.1.
PIRiS73475.
RefSeqiNP_109693.1. NC_000912.1.

3D structure databases

ProteinModelPortaliP75107.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP75107. 1 interaction.
STRINGi272634.MPN005.

Proteomic databases

PaxDbiP75107.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB95797; AAB95797; MPN_005.
GeneIDi877292.
KEGGimpn:MPN005.
PATRICi20021283. VBIMycPne110_0005.

Phylogenomic databases

eggNOGiCOG0172.
KOiK01875.
OMAiYRIVRIC.
OrthoDBiEOG61KBH9.

Enzyme and pathway databases

UniPathwayiUPA00906; UER00895.
BioCyciMPNE272634:GJ6Z-5-MONOMER.

Family and domain databases

Gene3Di1.10.287.40. 1 hit.
HAMAPiMF_00176. Ser_tRNA_synth_type1.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR002317. Ser-tRNA-ligase_type_1.
IPR015866. Ser-tRNA-synth_1_N.
IPR010978. tRNA-bd_arm.
[Graphical view]
PANTHERiPTHR11778. PTHR11778. 1 hit.
PfamiPF02403. Seryl_tRNA_N. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PIRSFiPIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTSiPR00981. TRNASYNTHSER.
SUPFAMiSSF46589. SSF46589. 1 hit.
TIGRFAMsiTIGR00414. serS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
    Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
    Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29342 / M129.

Entry informationi

Entry nameiSYS_MYCPN
AccessioniPrimary (citable) accession number: P75107
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 7, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.