Reviewed, UniProtKB/Swiss-Prot P75107 (SYS_MYCPN)

Names and origin

Protein names

Recommended name:
    Seryl-tRNA synthetase
    EC=6.1.1.11
Alternative name(s):
    Seryl-tRNA(Ser/Sec) synthetase
    Serine--tRNA ligase
      Short name=SerRS

Gene names

Name:	serS
Ordered Locus Names:	MPN_005
ORF Names:	MP149

Organism

Mycoplasma pneumoniae [Complete proteome] [HAMAP]

Taxonomic identifier

2104 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) By similarity.
Catalytic activity	ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser). HAMAP MF_00176 ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec). HAMAP MF_00176
Pathway	Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. HAMAP MF_00176
Subunit structure	Homodimer. The tRNA molecule binds across the dimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	selenocysteine biosynthetic process Inferred from electronic annotation. Source: HAMAP seryl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP serine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Seryl-tRNA synthetase HAMAP MF_00176		PRO_0000122083
Regions
	Nucleotide binding	256 – 258	3	ATP By similarity
	Nucleotide binding	343 – 346	4	ATP By similarity
	Region	225 – 227	3	Serine binding By similarity
Sites
	Binding site	279	1	Serine By similarity
	Binding site	379	1	Serine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P75107-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 9BC071823D20998E	FASTA	420	47,924
	10 20 30 40 50 60 MLDRNKLRNN LDFFKKKLVE RGVSESQFEA YVQADKAMRK LLHQIELANQ KQTLLAQQVA 70 80 90 100 110 120 KKKGDPKLLK ESKELKQKLE QLNIAFKEAE TLSQELLLNL PNIADESVPV GRDETANLEL 130 140 150 160 170 180 LKEGRKPVFD FTPLPHWELC ERLQLVAFDK ATKLTGARFV AYTDKAAKLL RAIASLMIDL 190 200 210 220 230 240 NKNKYQEWNV PVIVNETSLT GTGQLPKFKD DVFKLENTRY YLSPTLEVQL ANLHANEIFT 250 260 270 280 290 300 EGELPKYYTA TGVNFRQEAG SAGKQTKGTI RLHQFQKVEL VKFCKPSEAI HELEEMTRDA 310 320 330 340 350 360 EQILLELKIP FRRLLLCSGD MGFSAQKTYD LEVWMAGCNE YREVSSCSSC GDFQARRAMI 370 380 390 400 410 420 RYKDLTTGKN TYVATLNGTA LAIDRIFAAI LEHYQTKAGE VMIPQALLKY LDFDKITKPK

References

[1]

"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00089 Genomic DNA. Translation: AAB95797.1.
PIR	S73475.
RefSeq	NP_109693.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	877292.
GenomeReviews	Gene locus MPN_005 in contig U00089_GR.
KEGG	mpn:MPN005.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P75107.
OMA	EEWRELK
Enzyme and pathway databases
BioCyc	MPNE272634:MPN005-MON.
BRENDA	6.1.1.11. 39500.
Family and domain databases
HAMAP	MF_00176. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002317. Ser-tRNA-synth_IIa. IPR018156. Ser-tRNA-synth_IIa_C. IPR015866. Ser-tRNA-synth_IIa_N. IPR010978. tRNA_bd_arm. [Graphical view]
Gene3D	G3DSA:1.10.287.40. Ser-tRNA-synth_IIa_N. 1 hit.
PANTHER	PTHR11778. tRNA-synt_ser. 1 hit.
Pfam	PF02403. Seryl_tRNA_N. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTS	PR00981. TRNASYNTHSER.
TIGRFAMs	TIGR00414. serS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYS_MYCPN

Accession

Primary (citable) accession number: P75107

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	November 24, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)