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P75104 (MNME_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA modification GTPase MnmE
EC=3.6.-.-
Gene names
Name:mnmE
Synonyms:thdF, trmE
Ordered Locus Names:MPN_008
ORF Names:MP146
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length442 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity. HAMAP MF_00379

Cofactor

Binds 1 potassium ion per subunit By similarity. HAMAP MF_00379

Subunit structure

Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity. HAMAP MF_00379

Subcellular location

Cytoplasm By similarity HAMAP MF_00379.

Sequence similarities

Belongs to the Era/MnmE GTP-binding protein family. MnmE subfamily.

Contains 1 G (guanine nucleotide-binding) domain.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandGTP-binding
Magnesium
Metal-binding
Nucleotide-binding
Potassium
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processsmall GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

tRNA modification

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: InterPro

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

groLP780123EBI-2259625,EBI-2258565

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 442442tRNA modification GTPase MnmE HAMAP MF_00379
PRO_0000188892

Regions

Domain229 – 332104G
Nucleotide binding227 – 2326GTP By similarity
Nucleotide binding246 – 2527GTP By similarity
Nucleotide binding271 – 2744GTP By similarity
Nucleotide binding331 – 3344GTP By similarity

Sites

Metal binding2271Potassium By similarity
Metal binding2311Magnesium By similarity
Metal binding2461Potassium; via carbonyl oxygen By similarity
Metal binding2481Potassium; via carbonyl oxygen By similarity
Metal binding2511Potassium By similarity
Metal binding2521Magnesium By similarity
Binding site231Formyltetrahydrofolate By similarity
Binding site821Formyltetrahydrofolate By similarity
Binding site1211Formyltetrahydrofolate By similarity
Binding site4421Formyltetrahydrofolate By similarity

Sequences

Sequence LengthMass (Da)Tools
P75104 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: FA8849BADBCB9AD5

FASTA44249,780
        10         20         30         40         50         60 
MDTKQTMFAL ATAPFNSAIH IIRLSGPDVY RIINQITNKE VKPLGMRIQR VWLIDHNQKK 

        70         80         90        100        110        120 
VDDVLLFKFV APNSYTGEDL IEISCHGSMV IVNEIIGLLL KHGAVQAQPG EFTQRGYLNG 

       130        140        150        160        170        180 
KMSLNQAASV NNLVLSPNTT LKDVALNALA GQVDARLEPL VEKLGQLVMQ MEVNLDYPEY 

       190        200        210        220        230        240 
TDEQRELVTM NQAVVQITQI LNQIVVGQDQ LQRLKDPFKI AIIGNTNVGK SSLLNALLDQ 

       250        260        270        280        290        300 
DKAIVSAIKG STRDIVEGDF ALNGHFVKIL DTAGIRQHQS ALEKAGIQKT FGAIKTANLV 

       310        320        330        340        350        360 
IYLLDARQPE PDPKIIARLK KLKKDFFLVH NKADLVQQSF QVSISAKQKQ IQPLVDLLTQ 

       370        380        390        400        410        420 
YLHQFYSVEQ NQLYLISDWQ TILLQKAIAE LEHFLIKQQN CLFFDILVVH LRAAHEYILQ 

       430        440 
VLGKNTNYDL INEIFKHFCL GK

« Hide

References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U00089 Genomic DNA. Translation: AAB95794.1.
PIRS73472.
RefSeqNP_109696.1. NC_000912.1.

3D structure databases

ProteinModelPortalP75104.
ModBaseSearch...

Protein-protein interaction databases

IntActP75104. 2 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID877349.
GenomeReviewsGene locus MPN_008 in contig U00089_GR.
KEGGmpn:MPN008.
PATRIC20021289. VBIMycPne110_0008.

Phylogenomic databases

HOGENOMHBG746795.
OMAPGEFSEQ.
PhylomeDBP75104.
ProtClustDBPRK05291.

Enzyme and pathway databases

BioCycMPNE272634:MPN008-MONOMER.

Family and domain databases

HAMAPMF_00379. GTPase_MnmE.
[Tree]
InterProIPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_TrmE.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
Gene3DG3DSA:3.30.1360.120. GTP_bd_TrmE_N. 1 hit.
KOK03650.
PfamPF01926. MMR_HSR1. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
TIGRFAMsTIGR00450. MnmE_trmE_thdF. 1 hit.
TIGR00231. Small_GTP. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMNME_MYCPN
AccessionPrimary (citable) accession number: P75104
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names

SIMILARITY comments

Index of protein domains and families

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