P75091 (SYM_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	metG
Synonyms:	metS
Ordered Locus Names:	MPN_023
ORF Names:	MP131

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129)

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_01228
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_01228
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_01228
Subunit structure	Monomer By similarity. HAMAP MF_01228
Subcellular location	Cytoplasm HAMAP MF_01228.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	Methionine--tRNA ligase HAMAP MF_01228		PRO_0000139229
Regions
	Motif	11 – 21	11	"HIGH" region HAMAP MF_01228
	Motif	301 – 305	5	"KMSKS" region HAMAP MF_01228
Sites
	Metal binding	126	1	Zinc By similarity
	Metal binding	129	1	Zinc By similarity
	Metal binding	143	1	Zinc By similarity
	Metal binding	147	1	Zinc By similarity
	Binding site	304	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P75091 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: B5C3861CEC30A6AA	FASTA	512	59,263
	10 20 30 40 50 60 MKRCYITTPI YYASGKPHIG HAFTTILADV IKRYKQQNGY EAYFLTGTDE HGNKIESKAK 70 80 90 100 110 120 SLGLDPQTFV DQNVAYFQQM WKQLDINFDH FIRTTDLSHK AQVQHAFQLL YDKGLIYQSN 130 140 150 160 170 180 WEGAYCVECE QNYFTYDKQT MLCEIGHQLT LVQEPSLFIA FKDSKDWIGE MIATNKLNIT 190 200 210 220 230 240 PESRAAELKN NFLDGGLNDL ALTRQNVTWG IPVPFDNKQT IYVWFDALFN YITNLGFAHN 250 260 270 280 290 300 DPKFNKWWNN NDEEHEVIHL ISREITRFHC IYWPIFLHQL GFKLPTQFLS HGWIVDGNGH 310 320 330 340 350 360 KMSKSLGNVI SPEELLAQFG VDGTRYCLLK EMRLDKDNRC SMAIFKDIYN ADLANSFGNH 370 380 390 400 410 420 ASRTFGMIKK YLGGQLDFIE VQDPQVKQLM DQANQAMVQF DTAWNNFQFY KGINGLLQLV 430 440 450 460 470 480 FQASKLIDQL KPWELVKQTD YTLLKQLLFA CVRCTQVCFV LLAPILVHTS TQIFDLFNFS 490 500 510 AQARSKTHLA DPQQLQKISL APVIQPLFKR LD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.
[2]	"Towards a two-dimensional proteome map of Mycoplasma pneumoniae." Regula J.T., Ueberle B., Boguth G., Goerg A., Schnoelzer M., Herrmann R., Frank R. Electrophoresis 21:3765-3780(2000) [PubMed: 11271496] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: ATCC 29342 / M129.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB95779.1.
PIR	S73457.
RefSeq	NP_109711.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P75091.
ModBase	Search...
Protein-protein interaction databases
IntAct	P75091. 9 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	876981.
GenomeReviews	Gene locus MPN_023 in contig U00089_GR.
KEGG	mpn:MPN023.
PATRIC	20021321. VBIMycPne110_0022.
Phylogenomic databases
HOGENOM	HBG721667.
OMA	EEYKDEK.
PhylomeDB	P75091.
ProtClustDB	PRK11893.
Enzyme and pathway databases
BioCyc	MPNE272634:MPN023-MONOMER.
Family and domain databases
HAMAP	MF_01228. Met_tRNA_synth_type2. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR014758. Met-tRNA_synth. IPR023457. Met-tRNA_synth_2. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01874.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. MetG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_MYCPN

Accession

Primary (citable) accession number: P75091

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program