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P75089 (ALF_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fructose-bisphosphate aldolase

Short name=FBP aldolase
Short name=FBPA
EC=4.1.2.13
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene names
Name:fba
Synonyms:tsr
Ordered Locus Names:MPN_025
ORF Names:MP129
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length288 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis By similarity.

Catalytic activity

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactor

Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the class II fructose-bisphosphate aldolase family.

Ontologies

Keywords
   Biological processGlycolysis
   LigandMetal-binding
Zinc
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processfructose 1,6-bisphosphate metabolic process

Inferred from electronic annotation. Source: InterPro

glycolytic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionfructose-bisphosphate aldolase activity

Inferred from electronic annotation. Source: UniProtKB-EC

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 288288Fructose-bisphosphate aldolase
PRO_0000178720

Regions

Region207 – 2093Dihydroxyacetone phosphate binding By similarity
Region228 – 2314Dihydroxyacetone phosphate binding By similarity

Sites

Active site841Proton donor By similarity
Metal binding851Zinc 1; catalytic By similarity
Metal binding1051Zinc 2 By similarity
Metal binding1351Zinc 2 By similarity
Metal binding1771Zinc 1; catalytic By similarity
Metal binding2061Zinc 1; catalytic By similarity
Binding site491Glyceraldehyde 3-phosphate By similarity
Binding site1781Dihydroxyacetone phosphate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
P75089 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 174A7F2E4E215FD8

FASTA28831,069
        10         20         30         40         50         60 
MLVNIKQMLQ HAKQHHYAVP HININNYEWA KAVLTAAQQA KSPIIVSTSE GALKYISGHQ 

        70         80         90        100        110        120 
VVVPMVKGLV DALKITVPVA LHLDHGSYEG CKAALQAGFS SIMFDGSHLP FQENFTKSKE 

       130        140        150        160        170        180 
LIELAKQTNA SVELEVGTLG GEEDGIVGQG ELANIEECKQ IATLKPDALA AGIGNIHGLY 

       190        200        210        220        230        240 
PDNWKGLNYE LIEAIAKATN LPLVLHGGSG IPEADVKKAI GLGISKLNIN TECQLAFAKA 

       250        260        270        280 
IREYVEAKKD LDTHNKGYDP RKLLKSPTQA IVDCCLEKMQ LCGSTNKA 

« Hide

References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB95777.1.
PIRS73455.
RefSeqNP_109713.1. NC_000912.1.

3D structure databases

ProteinModelPortalP75089.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP75089. 7 interactions.
STRING272634.MPN025.

Proteomic databases

PaxDbP75089.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB95777; AAB95777; MPN_025.
GeneID876888.
KEGGmpn:MPN025.
PATRIC20021325. VBIMycPne110_0024.

Phylogenomic databases

eggNOGCOG0191.
KOK01624.
OMACTTRYEA.
OrthoDBEOG6HXJ7B.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-546.
MPNE272634:GJ6Z-27-MONOMER.
UniPathwayUPA00109; UER00183.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR011289. Fruc_bis_ald_class-2.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsTIGR00167. cbbA. 1 hit.
TIGR01859. fruc_bis_ald_. 1 hit.
PROSITEPS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALF_MYCPN
AccessionPrimary (citable) accession number: P75089
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: June 11, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names