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Protein

Fructose-bisphosphate aldolase

Gene

fba

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. One is catalytic and the other provides a structural contribution.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. Probable ATP-dependent 6-phosphofructokinase (pfkA)
  4. Fructose-bisphosphate aldolase (fba)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei49Glyceraldehyde 3-phosphateBy similarity1
Active sitei84Proton donorBy similarity1
Metal bindingi85Zinc 1; catalyticBy similarity1
Metal bindingi105Zinc 2By similarity1
Metal bindingi135Zinc 2By similarity1
Metal bindingi177Zinc 1; catalyticBy similarity1
Binding sitei178Dihydroxyacetone phosphate; via amide nitrogenBy similarity1
Metal bindingi206Zinc 1; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-546.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene namesi
Name:fba
Synonyms:tsr
Ordered Locus Names:MPN_025
ORF Names:MP129
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000808 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001787201 – 288Fructose-bisphosphate aldolaseAdd BLAST288

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP75089. 7 interactors.

Structurei

3D structure databases

ProteinModelPortaliP75089.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni207 – 209Dihydroxyacetone phosphate bindingBy similarity3
Regioni228 – 231Dihydroxyacetone phosphate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

KOiK01624.
OMAiEWLAIIR.

Family and domain databases

CDDicd00947. TBP_aldolase_IIB. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000771. FBA_II.
IPR011289. Fruc_bis_ald_class-2.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01859. fruc_bis_ald_. 1 hit.
PROSITEiPS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P75089-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVNIKQMLQ HAKQHHYAVP HININNYEWA KAVLTAAQQA KSPIIVSTSE
60 70 80 90 100
GALKYISGHQ VVVPMVKGLV DALKITVPVA LHLDHGSYEG CKAALQAGFS
110 120 130 140 150
SIMFDGSHLP FQENFTKSKE LIELAKQTNA SVELEVGTLG GEEDGIVGQG
160 170 180 190 200
ELANIEECKQ IATLKPDALA AGIGNIHGLY PDNWKGLNYE LIEAIAKATN
210 220 230 240 250
LPLVLHGGSG IPEADVKKAI GLGISKLNIN TECQLAFAKA IREYVEAKKD
260 270 280
LDTHNKGYDP RKLLKSPTQA IVDCCLEKMQ LCGSTNKA
Length:288
Mass (Da):31,069
Last modified:February 1, 1997 - v1
Checksum:i174A7F2E4E215FD8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB95777.1.
PIRiS73455.
RefSeqiNP_109713.1. NC_000912.1.
WP_010874382.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB95777; AAB95777; MPN_025.
GeneIDi876888.
KEGGimpn:MPN025.
PATRICi20021325. VBIMycPne110_0024.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB95777.1.
PIRiS73455.
RefSeqiNP_109713.1. NC_000912.1.
WP_010874382.1. NC_000912.1.

3D structure databases

ProteinModelPortaliP75089.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP75089. 7 interactors.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB95777; AAB95777; MPN_025.
GeneIDi876888.
KEGGimpn:MPN025.
PATRICi20021325. VBIMycPne110_0024.

Phylogenomic databases

KOiK01624.
OMAiEWLAIIR.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.
BioCyciMetaCyc:MONOMER-546.

Family and domain databases

CDDicd00947. TBP_aldolase_IIB. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000771. FBA_II.
IPR011289. Fruc_bis_ald_class-2.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01859. fruc_bis_ald_. 1 hit.
PROSITEiPS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALF_MYCPN
AccessioniPrimary (citable) accession number: P75089
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 30, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.