P75081 (UPP_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uracil phosphoribosyltransferase
EC=2.4.2.9

Alternative name(s):
UMP pyrophosphorylase
UPRTase

Gene names

Name:	upp
Ordered Locus Names:	MPN_033
ORF Names:	MP121

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129)

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate By similarity. HAMAP MF_01218_B
Catalytic activity	UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01218_B
Cofactor	Binds 1 Mg2+ ion per subunit. The magnesium is bound as Mg-PRPP By similarity.
Enzyme regulation	Allosterically activated by GTP By similarity. HAMAP MF_01218_B
Pathway	Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1. HAMAP MF_01218_B
Sequence similarities	Belongs to the UPRTase family.

Ontologies

Keywords
Ligand	GTP-binding Magnesium Nucleotide-binding
Molecular function	Glycosyltransferase Transferase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro uracil salvage Inferred from electronic annotation. Source: InterPro
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW uracil phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 206	206	Uracil phosphoribosyltransferase HAMAP MF_01218_B		PRO_0000120856
Regions
	Region	128 – 136	9	5-phospho-alpha-D-ribose 1-diphosphate binding By similarity
	Region	196 – 198	3	Uracil binding By similarity
Sites
	Binding site	76	1	5-phospho-alpha-D-ribose 1-diphosphate By similarity
	Binding site	101	1	5-phospho-alpha-D-ribose 1-diphosphate By similarity
	Binding site	191	1	Uracil; via amide nitrogen By similarity
	Binding site	197	1	5-phospho-alpha-D-ribose 1-diphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P75081 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 681506D3C62CC884	FASTA	206	22,750
	10 20 30 40 50 60 MIKKVHHALI ENELTKLRDK SSSSSQFRMA LSQITSLLFF EVTKESPLEE IVVKTPFTKT 70 80 90 100 110 120 KGYKLKNEIV LVPIMRAGLG MVDPIVRYSE KIRVGHLGIY REHEGTNVIS YYKKMPENIG 130 140 150 160 170 180 NSHVIILDPM LATGTTLATA ISSIKKNHPI KISVVAIVAA PEGIKAVEAA HPDVDIYLAA 190 200 IDEKLNKDNY IIPGLGDAGD RLFGTK

References

[1]	"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAG34738.1.
PIR	S73447.
RefSeq	NP_109721.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P75081.
SMR	P75081. Positions 4-206.
ModBase	Search...
Protein-protein interaction databases
IntAct	P75081. 8 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	877341.
GenomeReviews	Gene locus MPN_033 in contig U00089_GR.
KEGG	mpn:MPN033.
PATRIC	20021341. VBIMycPne110_0032.
Phylogenomic databases
HOGENOM	HBG326432.
OMA	VYVLDPM.
PhylomeDB	P75081.
ProtClustDB	PRK00129.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-569. MPNE272634:MPN033-MONOMER.
Family and domain databases
HAMAP	MF_01218_B. Upp_B. [Tree]
InterPro	IPR000836. PRibTrfase. IPR005765. Ura_phspho_trans. [Graphical view]
KO	K00761.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01091. Upp. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UPP_MYCPN

Accession

Primary (citable) accession number: P75081

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 30, 2000
Last modified:	January 25, 2012
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program