P75080 (DPO3_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase III polC-type
Short name=PolIII
EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	MPN_034
ORF Names:	MP120

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129)

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	1443 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity By similarity. HAMAP MF_00356
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00356
Subcellular location	Cytoplasm By similarity HAMAP MF_00356.
Sequence similarities	Belongs to the DNA polymerase type-C family. PolC subfamily. Contains 1 exonuclease domain.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
recA	P78014	3	EBI-2258713,EBI-2258718

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1443	1443	DNA polymerase III polC-type HAMAP MF_00356		PRO_0000204583
Regions
	Domain	408 – 567	160	Exonuclease

Sequences

	Sequence		Length	Mass (Da)
	P75080 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 5E8593E15B289C48	FASTA	1,443	165,102
	10 20 30 40 50 60 MVFDTETEKG KRIWALSQFL VKKNILDHNE LHQLNNRIEL VYLENDRENA LFLVALTLKK 70 80 90 100 110 120 PLTIDIWNAL YEGFQDAEGA ELRITFQEDA TFFKDGSTKS SVTLAIIKDY FKSFFGKDKK 130 140 150 160 170 180 YRILLEQELT HPNFLSYSNH ELKANCQSQE LDQWLIEQRQ AFIKWMHQAG FTHFGFVSLF 190 200 210 220 230 240 NPPAEKQLKV KSMKVSKYDK QFETEVFSTE FVPIHKINQQ MDEIKLMGQI FELKDFPGYN 250 260 270 280 290 300 NLRNTLNIYV TDFQLGGSLI LKWFYKDPKT IEGIKIGTWV KATVKVERDA KTQLLQGIIK 310 320 330 340 350 360 EISPIETPAY YRRPDQDKQK RVELVFHTKM SAFDGINSVQ EYAQFAKERD WKTIAVTDKD 370 380 390 400 410 420 NIHIYPTLYE VAKKYGLKAI YGLECNLIDD HIKIVSNPDK TKLKDATFVI FDIETTGLHG 430 440 450 460 470 480 RYDSVIEFAG IKVKHNREVE RMQFFLKIDG PLPAAVTEIT KITQAQLEDG MEQQAGLEKL 490 500 510 520 530 540 RAWLDGCVMV AHNGLSFDLP FLQTQFEKYN IAPLTNPLID TLALSWALNP GFASHTLSNI 550 560 570 580 590 600 CAKLKFDFDD ERLHRADYDT QALKKVFDYF KEQVELMGIT NLEQLDQELN QQCHFELLKR 610 620 630 640 650 660 TFTNTGIIYI KSQSGFAKLY ELLSIALTDN NATRPLVLTS TLQKFAKSFV ITDNPVQGDI 670 680 690 700 710 720 FKAALTKPLK ELEAAIKRVD FVLIAPPGAY AGYTIREGLK KEAIPNAIKL VVDTAQRLNK 730 740 750 760 770 780 LVAVASDAYF IHPWENEYYK AIVCAKGLGG RWHRHFNYKE REQRVPNVFV RTTGEMLNEM 790 800 810 820 830 840 SFLGEQLAYE LVVENTNKLA KQLTADDLVP VQTKLQPPVI EGSNENLAAK TWSQAKAIYG 850 860 870 880 890 900 DPLPKLIEQR IQEELKAIID NGFGIIYWIS HLLVKQSVQD GYFVGPRGSI GSSLVANLIG 910 920 930 940 950 960 ISEINPLVPH YLCESCQYFE VNEEVDDGYD LMVRDCPKCG AKAAFKGDGH NIPFATFMGF 970 980 990 1000 1010 1020 AGDKIPDIDL NFSSEYQAKA HAYVRELFGE QYTFRAGTIA TVAEKTAYGY ARNYFEIIKQ 1030 1040 1050 1060 1070 1080 TELATAPEIE RFKQKLVGIK RTTGQHPGGI MIFPNHKSVY EFTPCGYPAD DTSSDWKTTH 1090 1100 1110 1120 1130 1140 FEYDALGNTI LKLDILGQDD PTMLKHLGDL THVNPQNIPR FDKKLTEMFW SVNPLKLKPH 1150 1160 1170 1180 1190 1200 YLDEPTGAIG IPEFGTKFVR KILEQTKPKG FGDLIRVSGL SHGKNVWADN AQKILKDQNL 1210 1220 1230 1240 1250 1260 SLKDVIACRD DIMLYLIHKG MQAKDAFEIM EKVRKGIALN AKEVQLMQSN GVEQHWINSC 1270 1280 1290 1300 1310 1320 LKISYLFPKA HAAAYVLMAW RIAWFKLYHP LSYYACLLSF KLKEHDVSGF KSGVSFVKQK 1330 1340 1350 1360 1370 1380 LEELNTLYRI KRIKPKEAEL LTSYEVYLEM MARGIKLEQI SLTHSHATRF VEHNGMLIAP 1390 1400 1410 1420 1430 1440 FITIPGMGEA VANSIIEARN EKPFSSLDDF KKRTKITKKH IEAFTQMQLL DEFREQDNQK KLF

References

[1]

"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB95768.1.
PIR	S73446.
RefSeq	NP_109722.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P75080.
ModBase	Search...
Protein-protein interaction databases
IntAct	P75080. 4 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	877158.
GenomeReviews	Gene locus MPN_034 in contig U00089_GR.
KEGG	mpn:MPN034.
PATRIC	20021343. VBIMycPne110_0033.
Phylogenomic databases
HOGENOM	HBG617116.
OMA	WKTTHFD.
PhylomeDB	P75080.
ProtClustDB	PRK00448.
Enzyme and pathway databases
BioCyc	MPNE272634:MPN034-MONOMER.
Family and domain databases
HAMAP	MF_00356. DNApol_PolC. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR006054. DnaQ. IPR006055. Exonuclease. IPR013520. Exonuclease_RNaseT/DNA_pol3. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR006308. PolC_gram_pos. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K03763.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF00929. RNase_T. 1 hit. [Graphical view]
SMART	SM00479. EXOIII. 1 hit. SM00481. POLIIIAc. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
TIGRFAMs	TIGR00573. Dnaq. 1 hit. TIGR01405. PolC_Gram_pos. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DPO3_MYCPN

Accession

Primary (citable) accession number: P75080

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program