P75070 (KITH_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	tdk
Ordered Locus Names:	MPN_044
ORF Names:	MP110

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129)

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP MF_00124
Subunit structure	Homotetramer By similarity. HAMAP MF_00124
Subcellular location	Cytoplasm Potential HAMAP MF_00124.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 191	191	Thymidine kinase HAMAP MF_00124		PRO_0000174999
Regions
	Nucleotide binding	20 – 27	8	ATP By similarity
	Nucleotide binding	93 – 96	4	ATP By similarity
Sites
	Active site	94	1	Proton acceptor Potential
	Metal binding	150	1	Zinc By similarity
	Metal binding	153	1	Zinc By similarity
	Metal binding	183	1	Zinc By similarity
	Metal binding	186	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	P75070 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: C333AF82EFC21F15	FASTA	191	21,500
	10 20 30 40 50 60 MSFSQVFHQS PRGWIEVICG PMFSGKTEEL LRKIKRWKLA KIPVIIFKPK IDTRQQHLVK 70 80 90 100 110 120 SRNGHSDEAI EINSPLEIYD YLTKDRFDVV AIDEAQFFSS EIVEVVKSLN DLGINVIVSG 130 140 150 160 170 180 LDTDFRAEPF GSIPQLLAIA DKICKLDAVC NVCGQLAQRT QRIVSKSNET VLIGDIEAYE 190 PRCKLHQPSA G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.
[2]	"Towards a two-dimensional proteome map of Mycoplasma pneumoniae." Regula J.T., Ueberle B., Boguth G., Goerg A., Schnoelzer M., Herrmann R., Frank R. Electrophoresis 21:3765-3780(2000) [PubMed: 11271496] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB95758.1.
PIR	S73436.
RefSeq	NP_109732.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P75070.
ModBase	Search...
Protein-protein interaction databases
IntAct	P75070. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	877408.
GenomeReviews	Gene locus MPN_044 in contig U00089_GR.
KEGG	mpn:MPN044.
PATRIC	20021365. VBIMycPne110_0044.
Phylogenomic databases
HOGENOM	HBG522108.
OMA	EPRCRHH.
PhylomeDB	P75070.
ProtClustDB	PRK04296.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-566. MPNE272634:MPN044-MONOMER.
BRENDA	2.7.1.21. 3534.
Family and domain databases
HAMAP	MF_00124. Thymidine_kinase. [Tree]
InterPro	IPR001267. Thymidine_kinase. IPR020634. Thymidine_kinase_subgr. [Graphical view]
KO	K00857.
PANTHER	PTHR11441. TK_cell. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_MYCPN

Accession

Primary (citable) accession number: P75070

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program