P75068 (SYD_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	MPN_046
ORF Names:	MP108

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129)

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_0000110907

Sequences

	Sequence		Length	Mass (Da)
	P75068 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 65EBE73A85F8A424	FASTA	557	64,114
	10 20 30 40 50 60 MNLDNAKCFK QRVFIGNLTS EHLNKTVTIA GWVKRIKKLG ELNFVIVGDK TNTIQVTCKN 70 80 90 100 110 120 KEQVKYLTRE DLVIVKGKLK RLDSVRFEIT NPTITLFAKS KTPPLIIEDK TDALEEVRLR 130 140 150 160 170 180 YRYLDLRRPV MQKRLALRHK VTLAVRNWLD QMGFIEVETP TLTKSTPEGA RDFLVPARIR 190 200 210 220 230 240 EHSFYALPQS PQIYKQLLMV GGTEKYFQIA HVYRDEDSRK DRQPEHTQID LEVAFYTKEM 250 260 270 280 290 300 VMDLIQRLFV DVFRQVLNIK LKKPFPVLKF AEAFNRFGSD KPDLRYGFEL EDCTDLFQDS 310 320 330 340 350 360 PNQFTNLINA GGIVGGIQLP NLYLDEVSFK ALRKLAKDNG VSLEFYSDKA SSLKQPLDLP 370 380 390 400 410 420 LAGTILLVAH KSKTQAWTAL GAIRNELKYH LNLVKPNQYS FCWIVDFPLY EFDEKEQKWV 430 440 450 460 470 480 SAHNMFSNPQ PQWLVNFENH KAEALSEQYD LVLNGFELGS GSIRIHDPEV QTRLMQSLGV 490 500 510 520 530 540 DPQQFGFVME AYQYGAPVHA GMGLGLDRLM MIINNVDNIR EVMAFPKNAQ GIEMHTNAPD 550 QVDIKDITTI WSKHPVK

References

[1]	"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB95756.1.
PIR	S73434.
RefSeq	NP_109734.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P75068.
ModBase	Search...
Protein-protein interaction databases
IntAct	P75068. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	877157.
GenomeReviews	Gene locus MPN_046 in contig U00089_GR.
KEGG	mpn:MPN046.
PATRIC	20021369. VBIMycPne110_0046.
Phylogenomic databases
HOGENOM	HBG396032.
OMA	AFPKTQQ.
PhylomeDB	P75068.
ProtClustDB	PRK00476.
Enzyme and pathway databases
BioCyc	MPNE272634:MPN046-MONOMER.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_MYCPN

Accession

Primary (citable) accession number: P75068

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program