Reviewed, UniProtKB/Swiss-Prot P75068 (SYD_MYCPN)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	MPN_046
ORF Names:	MP108

Organism

Mycoplasma pneumoniae [Complete proteome] [HAMAP]

Taxonomic identifier

2104 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm HAMAP MF_00044.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	Aspartyl-tRNA synthetase HAMAP MF_00044		PRO_0000110907

Sequences

	Sequence		Length	Mass (Da)
	P75068-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 65EBE73A85F8A424	FASTA	557	64,114
	10 20 30 40 50 60 MNLDNAKCFK QRVFIGNLTS EHLNKTVTIA GWVKRIKKLG ELNFVIVGDK TNTIQVTCKN 70 80 90 100 110 120 KEQVKYLTRE DLVIVKGKLK RLDSVRFEIT NPTITLFAKS KTPPLIIEDK TDALEEVRLR 130 140 150 160 170 180 YRYLDLRRPV MQKRLALRHK VTLAVRNWLD QMGFIEVETP TLTKSTPEGA RDFLVPARIR 190 200 210 220 230 240 EHSFYALPQS PQIYKQLLMV GGTEKYFQIA HVYRDEDSRK DRQPEHTQID LEVAFYTKEM 250 260 270 280 290 300 VMDLIQRLFV DVFRQVLNIK LKKPFPVLKF AEAFNRFGSD KPDLRYGFEL EDCTDLFQDS 310 320 330 340 350 360 PNQFTNLINA GGIVGGIQLP NLYLDEVSFK ALRKLAKDNG VSLEFYSDKA SSLKQPLDLP 370 380 390 400 410 420 LAGTILLVAH KSKTQAWTAL GAIRNELKYH LNLVKPNQYS FCWIVDFPLY EFDEKEQKWV 430 440 450 460 470 480 SAHNMFSNPQ PQWLVNFENH KAEALSEQYD LVLNGFELGS GSIRIHDPEV QTRLMQSLGV 490 500 510 520 530 540 DPQQFGFVME AYQYGAPVHA GMGLGLDRLM MIINNVDNIR EVMAFPKNAQ GIEMHTNAPD 550 QVDIKDITTI WSKHPVK

References

[1]

"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00089 Genomic DNA. Translation: AAB95756.1.
PIR	S73434.
RefSeq	NP_109734.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	877157.
GenomeReviews	Gene locus MPN_046 in contig U00089_GR.
KEGG	mpn:MPN046.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG396032.
OMA	YQLDVEM.
Enzyme and pathway databases
BioCyc	MPNE272634:MPN046-MON.
BRENDA	6.1.1.12. 39500.
Family and domain databases
HAMAP	MF_00044. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002312. Asp-tRNA-synth_IIb. IPR020564. Asp-tRNA-synth_IIb_bac-type. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_MYCPN

Accession

Primary (citable) accession number: P75068

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	December 15, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)