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P75055

- TSAD_MYCPN

UniProt

P75055 - TSAD_MYCPN

Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Feb 1997)
      Previous versions | rss
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    Functioni

    Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

    Catalytic activityi

    L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

    Cofactori

    Binds 1 Fe2+ ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi110 – 1101IronUniRule annotation
    Metal bindingi114 – 1141IronUniRule annotation
    Binding sitei168 – 1681SubstrateUniRule annotation
    Binding sitei181 – 1811Substrate; via amide nitrogenUniRule annotation
    Binding sitei185 – 1851SubstrateUniRule annotation
    Binding sitei277 – 2771SubstrateUniRule annotation
    Metal bindingi301 – 3011IronUniRule annotation

    GO - Molecular functioni

    1. iron ion binding Source: UniProtKB-HAMAP
    2. metalloendopeptidase activity Source: InterPro
    3. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP

    GO - Biological processi

    1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciMPNE272634:GJ6Z-61-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
    Alternative name(s):
    N6-L-threonylcarbamoyladenine synthaseUniRule annotation
    Short name:
    t(6)A synthaseUniRule annotation
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
    tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
    Gene namesi
    Name:tsaDUniRule annotation
    Synonyms:gcp
    Ordered Locus Names:MPN_059
    ORF Names:MP095
    OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
    Taxonomic identifieri272634 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000808: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 319319tRNA N6-adenosine threonylcarbamoyltransferasePRO_0000096968Add
    BLAST

    Proteomic databases

    PaxDbiP75055.

    Interactioni

    Protein-protein interaction databases

    IntActiP75055. 2 interactions.
    STRINGi272634.MPN059.

    Structurei

    3D structure databases

    ProteinModelPortaliP75055.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni135 – 1395Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the KAE1 / TsaD family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0533.
    KOiK01409.
    OMAiEVEKNAH.
    OrthoDBiEOG6K402S.

    Family and domain databases

    HAMAPiMF_01445. TsaD.
    InterProiIPR000905. Gcp-like_dom.
    IPR017861. KAE1/YgjD.
    IPR017860. Peptidase_M22_CS.
    IPR022450. TsaD.
    [Graphical view]
    PfamiPF00814. Peptidase_M22. 1 hit.
    [Graphical view]
    PRINTSiPR00789. OSIALOPTASE.
    TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
    TIGR03723. T6A_YgjD. 1 hit.
    PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P75055-1 [UniParc]FASTAAdd to Basket

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    MEQPLCILGI ETTCDDTSIG VITESKVQAH IVLSSAKLHA QTGGVVPEVA    50
    ARSHEQNLLK ALQQSGVVLE QITHIAYAAN PGLPGCLHVG ATFARSLSFL 100
    LDKPLLPINH LYAHIFSALI DQDINQLKLP ALGLVVSGGH TAIYLIKSLF 150
    DLELIAETSD DAIGEVYDKV GRAMGFPYPA GPQLDSLFQP ELVKSHYFFR 200
    PSTKWTKFSY SGLKSQCFTK IKQLRERKGF NPQTHDWNEF ASNFQATIID 250
    HYINHVKDAI QQHQPQMLLL GGGVSANKYL REQVTQLQLP YLIAPLKYTS 300
    DNGAMIGFYA NLLINGKNN 319
    Length:319
    Mass (Da):35,210
    Last modified:February 1, 1997 - v1
    Checksum:i39021A8BBC6A95CE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB95743.1.
    PIRiS73421.
    RefSeqiNP_109747.1. NC_000912.1.

    Genome annotation databases

    EnsemblBacteriaiAAB95743; AAB95743; MPN_059.
    GeneIDi876963.
    KEGGimpn:MPN059.
    PATRICi20021395. VBIMycPne110_0059.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00089 Genomic DNA. Translation: AAB95743.1 .
    PIRi S73421.
    RefSeqi NP_109747.1. NC_000912.1.

    3D structure databases

    ProteinModelPortali P75055.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P75055. 2 interactions.
    STRINGi 272634.MPN059.

    Proteomic databases

    PaxDbi P75055.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAB95743 ; AAB95743 ; MPN_059 .
    GeneIDi 876963.
    KEGGi mpn:MPN059.
    PATRICi 20021395. VBIMycPne110_0059.

    Phylogenomic databases

    eggNOGi COG0533.
    KOi K01409.
    OMAi EVEKNAH.
    OrthoDBi EOG6K402S.

    Enzyme and pathway databases

    BioCyci MPNE272634:GJ6Z-61-MONOMER.

    Family and domain databases

    HAMAPi MF_01445. TsaD.
    InterProi IPR000905. Gcp-like_dom.
    IPR017861. KAE1/YgjD.
    IPR017860. Peptidase_M22_CS.
    IPR022450. TsaD.
    [Graphical view ]
    Pfami PF00814. Peptidase_M22. 1 hit.
    [Graphical view ]
    PRINTSi PR00789. OSIALOPTASE.
    TIGRFAMsi TIGR00329. gcp_kae1. 1 hit.
    TIGR03723. T6A_YgjD. 1 hit.
    PROSITEi PS01016. GLYCOPROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
      Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
      Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29342 / M129.

    Entry informationi

    Entry nameiTSAD_MYCPN
    AccessioniPrimary (citable) accession number: P75055
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: February 1, 1997
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Mycoplasma pneumoniae
      Mycoplasma pneumoniae (strain M129): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3