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P75055

- TSAD_MYCPN

UniProt

P75055 - TSAD_MYCPN

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Protein
tRNA N6-adenosine threonylcarbamoyltransferase
Gene
tsaD, gcp, MPN_059, MP095
Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction By similarity.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 Fe2+ ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi110 – 1101Iron By similarity
Metal bindingi114 – 1141Iron By similarity
Binding sitei168 – 1681Substrate By similarity
Binding sitei181 – 1811Substrate; via amide nitrogen By similarity
Binding sitei185 – 1851Substrate By similarity
Binding sitei277 – 2771Substrate By similarity
Metal bindingi301 – 3011Iron By similarity

GO - Molecular functioni

  1. iron ion binding Source: UniProtKB-HAMAP
  2. metalloendopeptidase activity Source: InterPro
  3. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP

GO - Biological processi

  1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMPNE272634:GJ6Z-61-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferase (EC:2.6.99.4)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD
tRNA threonylcarbamoyladenosine biosynthesis protein TsaD
Gene namesi
Name:tsaD
Synonyms:gcp
Ordered Locus Names:MPN_059
ORF Names:MP095
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000808: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation
PRO_0000096968Add
BLAST

Proteomic databases

PaxDbiP75055.

Interactioni

Protein-protein interaction databases

IntActiP75055. 2 interactions.
STRINGi272634.MPN059.

Structurei

3D structure databases

ProteinModelPortaliP75055.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni135 – 1395Substrate binding By similarity

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.

Phylogenomic databases

eggNOGiCOG0533.
KOiK01409.
OMAiEVEKNAH.
OrthoDBiEOG6K402S.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/YgjD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_YgjD. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P75055-1 [UniParc]FASTAAdd to Basket

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MEQPLCILGI ETTCDDTSIG VITESKVQAH IVLSSAKLHA QTGGVVPEVA    50
ARSHEQNLLK ALQQSGVVLE QITHIAYAAN PGLPGCLHVG ATFARSLSFL 100
LDKPLLPINH LYAHIFSALI DQDINQLKLP ALGLVVSGGH TAIYLIKSLF 150
DLELIAETSD DAIGEVYDKV GRAMGFPYPA GPQLDSLFQP ELVKSHYFFR 200
PSTKWTKFSY SGLKSQCFTK IKQLRERKGF NPQTHDWNEF ASNFQATIID 250
HYINHVKDAI QQHQPQMLLL GGGVSANKYL REQVTQLQLP YLIAPLKYTS 300
DNGAMIGFYA NLLINGKNN 319
Length:319
Mass (Da):35,210
Last modified:February 1, 1997 - v1
Checksum:i39021A8BBC6A95CE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00089 Genomic DNA. Translation: AAB95743.1.
PIRiS73421.
RefSeqiNP_109747.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB95743; AAB95743; MPN_059.
GeneIDi876963.
KEGGimpn:MPN059.
PATRICi20021395. VBIMycPne110_0059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00089 Genomic DNA. Translation: AAB95743.1 .
PIRi S73421.
RefSeqi NP_109747.1. NC_000912.1.

3D structure databases

ProteinModelPortali P75055.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P75055. 2 interactions.
STRINGi 272634.MPN059.

Proteomic databases

PaxDbi P75055.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAB95743 ; AAB95743 ; MPN_059 .
GeneIDi 876963.
KEGGi mpn:MPN059.
PATRICi 20021395. VBIMycPne110_0059.

Phylogenomic databases

eggNOGi COG0533.
KOi K01409.
OMAi EVEKNAH.
OrthoDBi EOG6K402S.

Enzyme and pathway databases

BioCyci MPNE272634:GJ6Z-61-MONOMER.

Family and domain databases

HAMAPi MF_01445. TsaD.
InterProi IPR000905. Gcp-like_dom.
IPR017861. KAE1/YgjD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view ]
Pfami PF00814. Peptidase_M22. 1 hit.
[Graphical view ]
PRINTSi PR00789. OSIALOPTASE.
TIGRFAMsi TIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_YgjD. 1 hit.
PROSITEi PS01016. GLYCOPROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
    Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
    Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29342 / M129.

Entry informationi

Entry nameiTSAD_MYCPN
AccessioniPrimary (citable) accession number: P75055
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: May 14, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi