Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi110 – 1101IronUniRule annotation
Metal bindingi114 – 1141IronUniRule annotation
Binding sitei168 – 1681SubstrateUniRule annotation
Binding sitei181 – 1811Substrate; via amide nitrogenUniRule annotation
Binding sitei185 – 1851SubstrateUniRule annotation
Binding sitei277 – 2771SubstrateUniRule annotation
Metal bindingi301 – 3011IronUniRule annotation

GO - Molecular functioni

  1. iron ion binding Source: UniProtKB-HAMAP
  2. metalloendopeptidase activity Source: InterPro
  3. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP

GO - Biological processi

  1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMPNE272634:GJ6Z-61-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
Ordered Locus Names:MPN_059
ORF Names:MP095
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000808: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319tRNA N6-adenosine threonylcarbamoyltransferasePRO_0000096968Add
BLAST

Proteomic databases

PaxDbiP75055.

Interactioni

Protein-protein interaction databases

IntActiP75055. 2 interactions.
STRINGi272634.MPN059.

Structurei

3D structure databases

ProteinModelPortaliP75055.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni135 – 1395Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0533.
KOiK01409.
OMAiIKQIAIG.
OrthoDBiEOG6K402S.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/YgjD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_YgjD. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P75055-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEQPLCILGI ETTCDDTSIG VITESKVQAH IVLSSAKLHA QTGGVVPEVA
60 70 80 90 100
ARSHEQNLLK ALQQSGVVLE QITHIAYAAN PGLPGCLHVG ATFARSLSFL
110 120 130 140 150
LDKPLLPINH LYAHIFSALI DQDINQLKLP ALGLVVSGGH TAIYLIKSLF
160 170 180 190 200
DLELIAETSD DAIGEVYDKV GRAMGFPYPA GPQLDSLFQP ELVKSHYFFR
210 220 230 240 250
PSTKWTKFSY SGLKSQCFTK IKQLRERKGF NPQTHDWNEF ASNFQATIID
260 270 280 290 300
HYINHVKDAI QQHQPQMLLL GGGVSANKYL REQVTQLQLP YLIAPLKYTS
310
DNGAMIGFYA NLLINGKNN
Length:319
Mass (Da):35,210
Last modified:February 1, 1997 - v1
Checksum:i39021A8BBC6A95CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB95743.1.
PIRiS73421.
RefSeqiNP_109747.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB95743; AAB95743; MPN_059.
GeneIDi876963.
KEGGimpn:MPN059.
PATRICi20021395. VBIMycPne110_0059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB95743.1.
PIRiS73421.
RefSeqiNP_109747.1. NC_000912.1.

3D structure databases

ProteinModelPortaliP75055.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP75055. 2 interactions.
STRINGi272634.MPN059.

Proteomic databases

PaxDbiP75055.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB95743; AAB95743; MPN_059.
GeneIDi876963.
KEGGimpn:MPN059.
PATRICi20021395. VBIMycPne110_0059.

Phylogenomic databases

eggNOGiCOG0533.
KOiK01409.
OMAiIKQIAIG.
OrthoDBiEOG6K402S.

Enzyme and pathway databases

BioCyciMPNE272634:GJ6Z-61-MONOMER.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/YgjD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_YgjD. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
    Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
    Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29342 / M129.

Entry informationi

Entry nameiTSAD_MYCPN
AccessioniPrimary (citable) accession number: P75055
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: January 7, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.