Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P75055 (TSAD_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA N6-adenosine threonylcarbamoyltransferase

EC=2.3.1.-
Alternative name(s):
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD
tRNA threonylcarbamoyladenosine biosynthesis protein TsaD
Gene names
Name:tsaD
Synonyms:gcp
Ordered Locus Names:MPN_059
ORF Names:MP095
OrganismMycoplasma pneumoniae (strain ATCC 29342 / M129) [Reference proteome] [HAMAP]
Taxonomic identifier272634 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction By similarity. HAMAP-Rule MF_01445

Cofactor

Binds 1 Fe2+ ion per subunit By similarity. HAMAP-Rule MF_01445

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01445.

Sequence similarities

Belongs to the KAE1 / TsaD family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandIron
Metal-binding
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processthreonylcarbamoyladenosine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioniron ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

transferase activity, transferring acyl groups other than amino-acyl groups

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 319319tRNA N6-adenosine threonylcarbamoyltransferase HAMAP-Rule MF_01445
PRO_0000096968

Regions

Region135 – 1395Substrate binding By similarity

Sites

Metal binding1101Iron By similarity
Metal binding1141Iron By similarity
Metal binding3011Iron By similarity
Binding site1681Substrate By similarity
Binding site1811Substrate; via amide nitrogen By similarity
Binding site1851Substrate By similarity
Binding site2771Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P75055 [UniParc].

Last modified February 1, 1997. Version 1.
Checksum: 39021A8BBC6A95CE

FASTA31935,210
        10         20         30         40         50         60 
MEQPLCILGI ETTCDDTSIG VITESKVQAH IVLSSAKLHA QTGGVVPEVA ARSHEQNLLK 

        70         80         90        100        110        120 
ALQQSGVVLE QITHIAYAAN PGLPGCLHVG ATFARSLSFL LDKPLLPINH LYAHIFSALI 

       130        140        150        160        170        180 
DQDINQLKLP ALGLVVSGGH TAIYLIKSLF DLELIAETSD DAIGEVYDKV GRAMGFPYPA 

       190        200        210        220        230        240 
GPQLDSLFQP ELVKSHYFFR PSTKWTKFSY SGLKSQCFTK IKQLRERKGF NPQTHDWNEF 

       250        260        270        280        290        300 
ASNFQATIID HYINHVKDAI QQHQPQMLLL GGGVSANKYL REQVTQLQLP YLIAPLKYTS 

       310 
DNGAMIGFYA NLLINGKNN 

« Hide

References

[1]"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."
Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.
Nucleic Acids Res. 24:4420-4449(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29342 / M129.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00089 Genomic DNA. Translation: AAB95743.1.
PIRS73421.
RefSeqNP_109747.1. NC_000912.1.

3D structure databases

ProteinModelPortalP75055.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP75055. 2 interactions.
STRING272634.MPN059.

Proteomic databases

PaxDbP75055.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAB95743; AAB95743; MPN_059.
GeneID876963.
KEGGmpn:MPN059.
PATRIC20021395. VBIMycPne110_0059.

Phylogenomic databases

eggNOGCOG0533.
KOK01409.
OMAIKQIAIG.
OrthoDBEOG6K402S.
ProtClustDBPRK09604.

Enzyme and pathway databases

BioCycMPNE272634:GJ6Z-61-MONOMER.

Family and domain databases

HAMAPMF_01445. TsaD.
InterProIPR000905. Gcp-like_dom.
IPR017861. KAE1/YgjD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view]
PfamPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSPR00789. OSIALOPTASE.
TIGRFAMsTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_YgjD. 1 hit.
PROSITEPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTSAD_MYCPN
AccessionPrimary (citable) accession number: P75055
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: February 19, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Mycoplasma pneumoniae

Mycoplasma pneumoniae (strain M129): entries and gene names