P75054 (SRP54_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal recognition particle protein

Alternative name(s):
Fifty-four homolog

Gene names

Name:	ffh
Ordered Locus Names:	MPN_061
ORF Names:	MP093

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129)

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Necessary for efficient export of extracytoplasmic proteins. Binds to the signal sequence when it emerges from the ribosomes By similarity.
Subunit structure	Signal recognition particle consists of a small cytoplasmic RNA (sc-RNA) molecule and protein ffh By similarity.
Domain	The protein has a two domain structure: the G-domain binds GTP; the M-domain binds the RNA and also binds the signal sequence.
Sequence similarities	Belongs to the GTP-binding SRP family.

Ontologies

Keywords
Cellular component	Signal recognition particle
Ligand	GTP-binding Nucleotide-binding RNA-binding
Molecular function	Ribonucleoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	SRP-dependent cotranslational protein targeting to membrane Inferred from electronic annotation. Source: InterPro
Cellular component	signal recognition particle, endoplasmic reticulum targeting Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	7S RNA binding Inferred from electronic annotation. Source: InterPro GTP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Signal recognition particle protein		PRO_0000101161
Regions
	Nucleotide binding	106 – 113	8	GTP By similarity
	Nucleotide binding	188 – 192	5	GTP By similarity
	Nucleotide binding	246 – 249	4	GTP By similarity
	Region	1 – 293	293	G-domain
	Region	294 – 450	157	M-domain

Sequences

	Sequence		Length	Mass (Da)
	P75054 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 7EFE42ECEA4FE679	FASTA	450	50,141
	10 20 30 40 50 60 MFKSMISSIV MRSMQKKINA QTISETDVQA VLKEIRIALL DADVNLLVVK NFIKAIREQT 70 80 90 100 110 120 VGQTVEPGQD LQKWLLKVIK QELINILSQP NQEITSKRPL KVMVVGLQGS GKTTTCGKLA 130 140 150 160 170 180 VWLKKQFQQK AMLVALDIYR PAAIDQLATL AEQTESVFFA KGTQAPDQTT KEAVKTFKES 190 200 210 220 230 240 GCQAIICDTA GRLQTNQELM DELVAIKNEL HPDEILMVVD GLSGQEIINV AKEFHNRLKL 250 260 270 280 290 300 TGFIITKLDS DARAGAALSL TSLLEVPIKL MGTSEKLTGL EQFHPERIAS RILGLGDVMT 310 320 330 340 350 360 LVEKAEEVFD KQSLTKTVSK MFLGKMDLED LLLYMEQMNQ MGSVSSIVKM LPGNLTVSDD 370 380 390 400 410 420 HVESIEAKVK LWKVLINSMT REERRHPKLI NRDPSRKQRI IKGSGRKMDE LNKLMKEWSK 430 440 450 LQTKTAEMGR MLKGGKNPFS GFGGLGGLGF

References

[1]	"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB95741.1.
PIR	S73419.
RefSeq	NP_109749.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P75054.
ModBase	Search...
Protein-protein interaction databases
IntAct	P75054. 4 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	876948.
GenomeReviews	Gene locus MPN_061 in contig U00089_GR.
KEGG	mpn:MPN061.
PATRIC	20021401. VBIMycPne110_0062.
Phylogenomic databases
HOGENOM	HBG562580.
OMA	IINGSRR.
PhylomeDB	P75054.
ProtClustDB	PRK10867.
Enzyme and pathway databases
BioCyc	MPNE272634:MPN061-MONOMER.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR000897. Signal_recog_part_SRP54_GTPase. IPR013822. Signal_recog_particl_SRP54_hlx. IPR004780. Signal_recog_particle_SRP. IPR004125. Signal_recog_particle_SRP54_M. [Graphical view]
Gene3D	G3DSA:1.20.120.140. SRP54_helical. 1 hit. G3DSA:1.10.260.30. SRP54_M. 1 hit.
KO	K03106.
PANTHER	PTHR11564:SF7. SRP_sub. 1 hit.
Pfam	PF00448. SRP54. 1 hit. PF02881. SRP54_N. 1 hit. PF02978. SRP_SPB. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00962. SRP54. 1 hit. SM00963. SRP54_N. 1 hit. [Graphical view]
SUPFAM	SSF47446. Signal_recog_particle_SRP54_M. 1 hit. SSF47364. SRP54. 1 hit.
TIGRFAMs	TIGR00959. Ffh. 1 hit.
PROSITE	PS00300. SRP54. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SRP54_MYCPN

Accession

Primary (citable) accession number: P75054

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	February 1, 1997
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program