Reviewed, UniProtKB/Swiss-Prot P75044 (KPRS_MYCPN)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase
      Short name=RPPK
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase
      Short name=P-Rib-PP synthetase
      Short name=PRPP synthetase

Gene names

Name:	prs
Synonyms:	prsA
Ordered Locus Names:	MPN_073
ORF Names:	MP082

Organism

Mycoplasma pneumoniae [Complete proteome] [HAMAP]

Taxonomic identifier

2104 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Ribose-phosphate pyrophosphokinase HAMAP MF_00583		PRO_0000141162
Regions
	Region	214 – 227	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	130	1	Magnesium Potential
	Metal binding	132	1	Magnesium Potential
	Metal binding	141	1	Magnesium Potential
	Metal binding	145	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	P75044-1 [UniParc]. Last modified October 25, 2002. Version 3. Checksum: 200FA5FB9648701D	FASTA	328	36,742
	10 20 30 40 50 60 MDRHNHVVFS LSKTHDLVSR ICQKLKMPMG LITHNEFADG ETYIRFEESV RNKDVFIFQS 70 80 90 100 110 120 TCAPVNDSLM ELLIAIDALK RGSAKSITAI LPYYGYARQD RKTMGREPIT SKLVADLLTT 130 140 150 160 170 180 AGVSRVALTD IHSDQTQGFF NIPVDTLRTY HVFLTRTVEL LGKKDLVVVS PDYGGVKRAR 190 200 210 220 230 240 LIATSLELPL AIIDKRRPAH NVAESINVLG EVANKNCLIV DDMIDTGGTV IAAAKLLREH 250 260 270 280 290 300 HAKKVCVMAT HGLFNGEAPQ RFQKAFNEGL VDYLFVSNSI PQTKFDQCPQ FQVIDLAPLF 310 320 EEVLLCYANN SSISAIYTRH IEWIKKHV

References

[1]

"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00089 Genomic DNA. Translation: AAB95730.1. Different initiation.
PIR	S73408.
RefSeq	NP_109761.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	877326.
GenomeReviews	Gene locus MPN_073 in contig U00089_GR.
KEGG	mpn:MPN073.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P75044.
OMA	CATHAVF
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-576. MPNE272634:MPN073-MON.
BRENDA	2.7.6.1. 39500.
Family and domain databases
HAMAP	MF_00583. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_MYCPN

Accession

Primary (citable) accession number: P75044

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 25, 2002
Last modified:	November 24, 2009
This is version 58 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)