P75044 (KPRS_MYCPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase
Short name=RPPK
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase
Short name=P-Rib-PP synthase
Short name=PRPP synthase

Gene names

Name:	prs
Synonyms:	prsA
Ordered Locus Names:	MPN_073
ORF Names:	MP082

Organism

Mycoplasma pneumoniae (strain ATCC 29342 / M129)

Taxonomic identifier

272634 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583_B
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00583_B
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00583_B.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.
Sequence caution	The sequence AAB95730.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Ribose-phosphate pyrophosphokinase HAMAP MF_00583_B		PRO_0000141162
Regions
	Region	214 – 227	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	130	1	Magnesium Potential
	Metal binding	132	1	Magnesium Potential
	Metal binding	141	1	Magnesium Potential
	Metal binding	145	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	P75044 [UniParc]. Last modified October 25, 2002. Version 3. Checksum: 200FA5FB9648701D	FASTA	328	36,742
	10 20 30 40 50 60 MDRHNHVVFS LSKTHDLVSR ICQKLKMPMG LITHNEFADG ETYIRFEESV RNKDVFIFQS 70 80 90 100 110 120 TCAPVNDSLM ELLIAIDALK RGSAKSITAI LPYYGYARQD RKTMGREPIT SKLVADLLTT 130 140 150 160 170 180 AGVSRVALTD IHSDQTQGFF NIPVDTLRTY HVFLTRTVEL LGKKDLVVVS PDYGGVKRAR 190 200 210 220 230 240 LIATSLELPL AIIDKRRPAH NVAESINVLG EVANKNCLIV DDMIDTGGTV IAAAKLLREH 250 260 270 280 290 300 HAKKVCVMAT HGLFNGEAPQ RFQKAFNEGL VDYLFVSNSI PQTKFDQCPQ FQVIDLAPLF 310 320 EEVLLCYANN SSISAIYTRH IEWIKKHV

References

[1]

"Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae." Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R. Nucleic Acids Res. 24:4420-4449(1996) [PubMed: 8948633] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29342 / M129.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00089 Genomic DNA. Translation: AAB95730.1. Different initiation.
PIR	S73408.
RefSeq	NP_109761.1. NC_000912.1.
3D structure databases
ProteinModelPortal	P75044.
ModBase	Search...
Protein-protein interaction databases
IntAct	P75044. 2 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	877326.
GenomeReviews	Gene locus MPN_073 in contig U00089_GR.
KEGG	mpn:MPN073.
PATRIC	20021425. VBIMycPne110_0074.
Phylogenomic databases
HOGENOM	HBG519284.
OMA	CATHAVF.
PhylomeDB	P75044.
ProtClustDB	PRK00553.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-576. MPNE272634:MPN073-MONOMER.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B. [Tree]
InterPro	IPR000842. PRib_PP_synth_CS. IPR000836. PRibTrfase. IPR005946. Rib-P_diPkinase. [Graphical view]
KO	K00948.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. RibP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_MYCPN

Accession

Primary (citable) accession number: P75044

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 25, 2002
Last modified:	January 25, 2012
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program