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Protein

PTS system fructose-specific EIIABC component

Gene

fruA

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in fructose transport.By similarity

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-fructose(Side 1) = [protein]-L-histidine + D-fructose 1-phosphate(Side 2).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei68Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation1
Active sitei185Phosphocysteine intermediate; for EIIB activityCurated1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system fructose-specific EIIABC componentBy similarity
Alternative name(s):
EIIABC-FruBy similarity
Including the following 3 domains:
PTS system fructose-specific EIIA componentBy similarity (EC:2.7.1.202By similarity)
Alternative name(s):
EII-FruBy similarity
Fructose-specific phosphotransferase enzyme IIA componentBy similarity
PTS system fructose-specific EIIB componentBy similarity (EC:2.7.1.202By similarity)
Alternative name(s):
EIII-FruBy similarity
Fructose-specific phosphotransferase enzyme IIB componentBy similarity
PTS system fructose-specific EIIC componentBy similarity
Alternative name(s):
Fructose permease IIC componentBy similarity
Gene namesi
Name:fruABy similarity
Ordered Locus Names:MPN_078
ORF Names:MP077
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000808 Componenti: Chromosome

Subcellular locationi

  • Cell membrane PROSITE-ProRule annotation; Multi-pass membrane protein PROSITE-ProRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei318 – 338HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei364 – 384HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei390 – 410HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei422 – 442HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei461 – 481HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei502 – 522HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei542 – 562HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei576 – 596HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei602 – 622HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei655 – 675HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001865111 – 694PTS system fructose-specific EIIABC componentAdd BLAST694

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei68Phosphohistidine; by HPrCurated1
Modified residuei185Phosphocysteine; by EIIAPROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliP75039.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 149PTS EIIA type-2PROSITE-ProRule annotationAdd BLAST146
Domaini179 – 275PTS EIIB type-2PROSITE-ProRule annotationAdd BLAST97
Domaini310 – 687PTS EIIC type-2PROSITE-ProRule annotationAdd BLAST378

Domaini

The PTS EIIA type-2 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-2 domain.PROSITE-ProRule annotation
The PTS EIIB type-2 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-2 domain.PROSITE-ProRule annotation
The EIIC type-2 domain forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 PTS EIIA type-2 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIB type-2 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIC type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK02768.
K02769.
K02770.
OMAiIPFVSEP.

Family and domain databases

CDDicd05569. PTS_IIB_fructose. 1 hit.
Gene3Di3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
IPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR004715. PTS_IIA_fruc.
IPR003353. PTS_IIB_fruc.
IPR006327. PTS_IIC_fruc.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
PF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
SSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00829. FRU. 1 hit.
TIGR00848. fruA. 1 hit.
TIGR01427. PTS_IIC_fructo. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P75039-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKPLLSAEL FFNWTAKDFK DKTSFLKQAC RVLQDKNCIK EEQIALTALK
60 70 80 90 100
EREAQITTGI MSKLALPHMQ SATVLKPFVA VFKVNNVDWQ SLDNQPVKLI
110 120 130 140 150
FLIGVPKDQG NLHLEFISQF SKLMLQDEFA NKVPNIRSFN GLINLIDSFQ
160 170 180 190 200
QTAVASQPVV NEAAAQTEEP KDTNTQYDFV AVTACPTGIA HTFMAKEALE
210 220 230 240 250
KFARDHNLKV KVETQGTDGI QNQLTESDLN NTKGIILACD RLIDLTRFYG
260 270 280 290 300
HANVVEVSTT KAIKTPQTVY DQVVKKEGKL LGNKSSDSAS QTELKETTEQ
310 320 330 340 350
LSFKDFHKRI YRAILSGVSY MLPFVVFGGI LIAIAFLIDI NNAGNAGKQF
360 370 380 390 400
GSKDPIANWF KTLGGLSFGL IVPILSAYIA FALVGRQGLL PGFIVGLISA
410 420 430 440 450
GKFLLNIDIV TGKIDWATES KVSSGFFGAI FGGLLAAVLI IVQQRYIYRK
460 470 480 490 500
LPQALQGIKN ILFIPLLGTL VTAALFWVIN IPLIYLNYGL SKFLQIMDKP
510 520 530 540 550
YLAPLLGLVI GLMMCFDLGG PVNKAAYVFG VVSLESQNSG TVAMASAILS
560 570 580 590 600
GMVPPLGIAI AATIRKQCFD KEELPAAYAC YVMGLSFISE GAIPFVAKRP
610 620 630 640 650
KIMLAANLIG GAVCGVLTGA FALTIRAPHG GVFVFALLKT NLEGIAGNTL
660 670 680 690
QIGAGVGLAL LALIVSSFIS AGIIIGHNLL VVRKKTKQLV NTNA
Length:694
Mass (Da):75,164
Last modified:February 1, 1997 - v1
Checksum:i919E12BFBCC5FF6A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB95725.1.
PIRiS73403.
RefSeqiNP_109766.1. NC_000912.1.
WP_010874435.1. NC_000912.1.

Genome annotation databases

EnsemblBacteriaiAAB95725; AAB95725; MPN_078.
GeneIDi877051.
KEGGimpn:MPN078.
PATRICi20021437. VBIMycPne110_0079.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA. Translation: AAB95725.1.
PIRiS73403.
RefSeqiNP_109766.1. NC_000912.1.
WP_010874435.1. NC_000912.1.

3D structure databases

ProteinModelPortaliP75039.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB95725; AAB95725; MPN_078.
GeneIDi877051.
KEGGimpn:MPN078.
PATRICi20021437. VBIMycPne110_0079.

Phylogenomic databases

KOiK02768.
K02769.
K02770.
OMAiIPFVSEP.

Family and domain databases

CDDicd05569. PTS_IIB_fructose. 1 hit.
Gene3Di3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
IPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR004715. PTS_IIA_fruc.
IPR003353. PTS_IIB_fruc.
IPR006327. PTS_IIC_fruc.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
PF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
SUPFAMiSSF52794. SSF52794. 1 hit.
SSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00829. FRU. 1 hit.
TIGR00848. fruA. 1 hit.
TIGR01427. PTS_IIC_fructo. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTF3A_MYCPN
AccessioniPrimary (citable) accession number: P75039
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: November 2, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.