ID   PXCA_SYNY3              Reviewed;         440 AA.
AC   P75028;
DT   01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   27-MAR-2024, entry version 130.
DE   RecName: Full=Proton extrusion protein PxcA {ECO:0000255|HAMAP-Rule:MF_01308, ECO:0000303|PubMed:9683474};
GN   Name=pxcA {ECO:0000255|HAMAP-Rule:MF_01308,
GN   ECO:0000303|PubMed:9683474};
GN   Synonyms=cotA {ECO:0000303|PubMed:9190798};
GN   OrderedLocusNames=slr1596 {ECO:0000303|PubMed:32619428};
OS   Synechocystis sp. (strain PCC 6803 / Kazusa).
OC   Bacteria; Cyanobacteriota; Cyanophyceae; Synechococcales; Merismopediaceae;
OC   Synechocystis.
OX   NCBI_TaxID=1111708;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8633006; DOI=10.1073/pnas.93.9.4006;
RA   Katoh A., Lee K.S., Fukuzawa H., Ohyama K., Ogawa T.;
RT   "cemA homologue essential to CO2 transport in the cyanobacterium
RT   Synechocystis PCC6803.";
RL   Proc. Natl. Acad. Sci. U.S.A. 93:4006-4010(1996).
RN   [2] {ECO:0000312|EMBL:BAA09896.1}
RP   SEQUENCE REVISION TO N-TERMINUS, SUBCELLULAR LOCATION, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=9190798; DOI=10.1128/jb.179.12.3845-3850.1997;
RA   Sonoda M., Kitano K., Katoh A., Katoh H., Ohkawa H., Ogawa T.;
RT   "Size of cotA and identification of the gene product in Synechocystis sp.
RT   strain PCC6803.";
RL   J. Bacteriol. 179:3845-3850(1997).
RN   [3] {ECO:0000312|EMBL:BAA16993.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA   Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA   Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA   Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA   Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence analysis of the genome of the unicellular cyanobacterium
RT   Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT   genome and assignment of potential protein-coding regions.";
RL   DNA Res. 3:109-136(1996).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=8808935; DOI=10.1128/jb.178.18.5452-5455.1996;
RA   Katoh A., Sonoda M., Katoh H., Ogawa T.;
RT   "Absence of light-induced proton extrusion in a cotA-less mutant of
RT   Synechocystis sp. strain PCC6803.";
RL   J. Bacteriol. 178:5452-5455(1996).
RN   [5]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=9683474; DOI=10.1128/jb.180.15.3799-3803.1998;
RA   Sonoda M., Katoh H., Vermaas W., Schmetterer G., Ogawa T.;
RT   "Photosynthetic electron transport involved in PxcA-dependent proton
RT   extrusion in Synechocystis sp. Strain PCC6803: effect of pxcA inactivation
RT   on CO2, HCO3-, and NO3- uptake.";
RL   J. Bacteriol. 180:3799-3803(1998).
RN   [6]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=PCC 6803 / Kazusa;
RX   PubMed=32619428; DOI=10.1016/j.bbabio.2020.148258;
RA   Inago H., Sato R., Masuda S.;
RT   "Regulation of light-induced H+ extrusion and uptake by cyanobacterial
RT   homologs of the plastidial FLAP1, DLDG1, and Ycf10 in Synechocystis sp.
RT   PCC6803.";
RL   Biochim. Biophys. Acta 1861:148258-148258(2020).
CC   -!- FUNCTION: Required for H(+) efflux immediately after light irradiation
CC       to form a rapid H(+) concentration gradient across the thylakoid
CC       membranes (PubMed:32619428). Together with PxcL, contributes to
CC       transient H(+) uptake following dark to light transition
CC       (PubMed:32619428, PubMed:8633006, PubMed:8808935, PubMed:9683474) (By
CC       similarity). Involved in light-induced Na(+)-dependent proton extrusion
CC       (PubMed:8808935, PubMed:9683474). Also seems to be involved in CO(2)
CC       transport (PubMed:8633006, PubMed:32619428). {ECO:0000255|HAMAP-
CC       Rule:MF_01308, ECO:0000269|PubMed:32619428, ECO:0000269|PubMed:8633006,
CC       ECO:0000269|PubMed:8808935, ECO:0000269|PubMed:9683474}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01308, ECO:0000269|PubMed:9190798}; Multi-pass membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_01308, ECO:0000305|PubMed:9190798}.
CC   -!- DISRUPTION PHENOTYPE: Loss of light-induced proton extrusion, grows
CC       poorly at low pH or in low Na(+) medium (PubMed:8633006,
CC       PubMed:8808935, PubMed:9190798, PubMed:9683474). Relaxed light-induced
CC       H(+) extrusion activity. H(+) influx activity is also partially
CC       diminished (PubMed:32619428). The double mutant missing both PxcA and
CC       PxcL is impaired for light-induced H(+) extrusion and later exhibits
CC       lower H(+) uptake activity (PubMed:32619428). The double mutant missing
CC       both PxcA and FlpA has both reduced light-induced H(+) extrusion and
CC       later lower H(+) uptake activity (PubMed:32619428, PubMed:8633006,
CC       PubMed:9190798, PubMed:8808935, PubMed:9683474).
CC       {ECO:0000269|PubMed:32619428, ECO:0000269|PubMed:8633006,
CC       ECO:0000269|PubMed:8808935, ECO:0000269|PubMed:9190798,
CC       ECO:0000269|PubMed:9683474}.
CC   -!- SIMILARITY: Belongs to the CemA family. {ECO:0000255|HAMAP-
CC       Rule:MF_01308}.
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DR   EMBL; D63838; BAA09896.1; -; Genomic_DNA.
DR   EMBL; BA000022; BAA16993.1; -; Genomic_DNA.
DR   PIR; S74953; S74953.
DR   AlphaFoldDB; P75028; -.
DR   IntAct; P75028; 16.
DR   STRING; 1148.gene:10497854; -.
DR   TCDB; 9.B.73.1.2; the chloroplast envelope/cyanobacterial membrane protein (cema) family.
DR   PaxDb; 1148-1652068; -.
DR   EnsemblBacteria; BAA16993; BAA16993; BAA16993.
DR   KEGG; syn:slr1596; -.
DR   eggNOG; ENOG502Z8DN; Bacteria.
DR   InParanoid; P75028; -.
DR   PhylomeDB; P75028; -.
DR   Proteomes; UP000001425; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015078; F:proton transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01308; CemA_PxcA; 1.
DR   InterPro; IPR004282; CemA.
DR   PANTHER; PTHR33650:SF2; CHLOROPLAST ENVELOPE MEMBRANE PROTEIN; 1.
DR   PANTHER; PTHR33650; CHLOROPLAST ENVELOPE MEMBRANE PROTEIN-RELATED; 1.
DR   Pfam; PF03040; CemA; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Hydrogen ion transport; Ion transport;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..440
FT                   /note="Proton extrusion protein PxcA"
FT                   /id="PRO_0000216670"
FT   TRANSMEM        222..242
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01308"
FT   TRANSMEM        316..336
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01308"
FT   TRANSMEM        352..374
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01308"
FT   TRANSMEM        400..420
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01308"
SQ   SEQUENCE   440 AA;  51117 MW;  67105B2ABEB13452 CRC64;
     MDLTNWWQGA TQWFGRSSQK SLEQAFRSAL KIKEIEDQYF QGKKIGPENC DYSADTVTYF
     ANQIQRHLRK IEQEIYHLNS DQEFVKILSL DPAVKQDPQT EYVLNQLQFI DDILQRYDGE
     LPQVSPPKQI ANGGVLDLPA ITANKQRQIN KKRRDGFQYI RREDTQQKVD TATQKSGVLP
     RSFLRTIDRL KREMDPQSSD TEQKVLKQYR NSRYKTALSI KFVLTLIIVP LLAHQLTKTF
     FLLPSVESFF ERNSEVVFIN QSMETEAYEE LSHFEESLRF RELLGFGEKL SPEAKEEKLA
     EKAKEISESY RRVSTNAIAN IFADIFSLVA FSLVLVNSQR EIEVLKEFID EIVYGLSDSA
     KAFLIILFTD MFVGFHSPHG WEVILASIAR HFGLPENQDF NFLFIATFPV ILDTVFKYWI
     FRYLNSISPS AVATYRNMNE
//