P75028 (PCXA_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proton extrusion protein PcxA

Gene names

Name:	pcxA
Synonyms:	cotA
Ordered Locus Names:	slr1596

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Synechocystis › Synechocystis sp. PCC 6803

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in light-induced Na+-dependent proton extrusion. Also seems to be involved in CO2 transport. HAMAP-Rule MF_01308
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.2.
Sequence similarities	Belongs to the Cema family.

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hydrogen ion transmembrane transporter activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Proton extrusion protein PcxA HAMAP-Rule MF_01308		PRO_0000216670
Regions
	Transmembrane	217 – 236	20	Helical; Potential
	Transmembrane	317 – 339	23	Helical; Potential
	Transmembrane	352 – 374	23	Helical; Potential
	Transmembrane	402 – 421	20	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P75028 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 67105B2ABEB13452	FASTA	440	51,117
	10 20 30 40 50 60 MDLTNWWQGA TQWFGRSSQK SLEQAFRSAL KIKEIEDQYF QGKKIGPENC DYSADTVTYF 70 80 90 100 110 120 ANQIQRHLRK IEQEIYHLNS DQEFVKILSL DPAVKQDPQT EYVLNQLQFI DDILQRYDGE 130 140 150 160 170 180 LPQVSPPKQI ANGGVLDLPA ITANKQRQIN KKRRDGFQYI RREDTQQKVD TATQKSGVLP 190 200 210 220 230 240 RSFLRTIDRL KREMDPQSSD TEQKVLKQYR NSRYKTALSI KFVLTLIIVP LLAHQLTKTF 250 260 270 280 290 300 FLLPSVESFF ERNSEVVFIN QSMETEAYEE LSHFEESLRF RELLGFGEKL SPEAKEEKLA 310 320 330 340 350 360 EKAKEISESY RRVSTNAIAN IFADIFSLVA FSLVLVNSQR EIEVLKEFID EIVYGLSDSA 370 380 390 400 410 420 KAFLIILFTD MFVGFHSPHG WEVILASIAR HFGLPENQDF NFLFIATFPV ILDTVFKYWI 430 440 FRYLNSISPS AVATYRNMNE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cemA homologue essential to CO2 transport in the cyanobacterium Synechocystis PCC6803." Katoh A., Lee K.S., Fukuzawa H., Ohyama K., Ogawa T. Proc. Natl. Acad. Sci. U.S.A. 93:4006-4010(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Size of cotA and identification of the gene product in Synechocystis sp. strain PCC6803." Sonoda M., Kitano K., Katoh A., Katoh H., Ohkawa H., Ogawa T. J. Bacteriol. 179:3845-3850(1997) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO N-TERMINUS, SUBCELLULAR LOCATION.
[3]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 6803 / Kazusa.
[4]	"Absence of light-induced proton extrusion in a cotA-less mutant of Synechocystis sp. strain PCC6803." Katoh A., Sonoda M., Katoh H., Ogawa T. J. Bacteriol. 178:5452-5455(1996) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Photosynthetic electron transport involved in PxcA-dependent proton extrusion in Synechocystis sp. Strain PCC6803: effect of pxcA inactivation on CO2, HCO3-, and NO3- uptake." Sonoda M., Katoh H., Vermaas W., Schmetterer G., Ogawa T. J. Bacteriol. 180:3799-3803(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D63838 Genomic DNA. Translation: BAA09896.1. BA000022 Genomic DNA. Translation: BAA16993.1.
PIR	S74953.
RefSeq	NP_440313.1. NC_000911.1. YP_005650370.1. NC_017277.1. YP_007450196.1. NC_020286.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P75028. 16 interactions.
STRING	1148.slr1596.
Protein family/group databases
TCDB	9.B.73.1.2. chloroplast envelope/cyanobacterial membrane protein (CemA) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAA16993; BAA16993; BAA16993.
GeneID	12256060. 14615844. 953612.
KEGG	syn:slr1596. syy:SYNGTS_0417.
PATRIC	23837766. VBISynSp132158_0452.
Phylogenomic databases
eggNOG	NOG06592.
HOGENOM	HOG000037065.
OMA	ILFTDVF.
ProtClustDB	PRK02507.
Family and domain databases
HAMAP	MF_01308. CemA.
InterPro	IPR004282. CemA. [Graphical view]
Pfam	PF03040. CemA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCXA_SYNY3

Accession

Primary (citable) accession number: P75028

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	February 1, 1997
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program