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Protein

Lon protease

Gene

lon

Organism
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner (By similarity). Regulates swarmer cell differentiation of V.parahaemolyticus.UniRule annotation

Catalytic activityi

Hydrolysis of proteins in presence of ATP.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei678 – 6781UniRule annotation
Active sitei721 – 7211UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi355 – 3628ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciVPAR223926:GHK4-961-MONOMER.

Protein family/group databases

MEROPSiS16.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Lon proteaseUniRule annotation (EC:3.4.21.53UniRule annotation)
Alternative name(s):
ATP-dependent protease LaUniRule annotation
Gene namesi
Name:lonUniRule annotation
Synonyms:lonS
Ordered Locus Names:VP0919
OrganismiVibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Taxonomic identifieri223926 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000002493 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 783783Lon proteasePRO_0000076147Add
BLAST

Proteomic databases

PRIDEiP74956.

Expressioni

Inductioni

By heat shock.UniRule annotation

Interactioni

Subunit structurei

Homohexamer. Organized in a ring with a central cavity.UniRule annotation

Protein-protein interaction databases

STRINGi223926.VP0919.

Structurei

3D structure databases

ProteinModelPortaliP74956.
SMRiP74956. Positions 8-116, 490-583, 593-783.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 201191Lon N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini591 – 772182Lon proteolyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S16 family.UniRule annotation
Contains 1 Lon N-terminal domain.PROSITE-ProRule annotation
Contains 1 Lon proteolytic domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105C6P. Bacteria.
COG0466. LUCA.
HOGENOMiHOG000261410.
KOiK01338.
OMAiGIKKAGM.
OrthoDBiEOG6XHC23.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01973. lon_bact.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027543. Lon_bac.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR003111. LON_substr-bd_dom.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF05362. Lon_C. 1 hit.
PF02190. LON_substr_bdg. 1 hit.
[Graphical view]
PIRSFiPIRSF001174. Lon_proteas. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00763. lon. 1 hit.
PROSITEiPS51787. LON_N. 1 hit.
PS51786. LON_PROTEOLYTIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P74956-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLERSERIE IPVLPLRDVV VYPHMVIPLF VGREKSISCL ETAMETNKQV
60 70 80 90 100
LLVAQKQADT DEPTVDDLFE VGTVATILQL LKLPDGTVKV LVEGQQRAKI
110 120 130 140 150
NHFKESDFFL AEAEFIVTPE LDEREQEVIV RSAINQFEGF IKLNKKIPPE
160 170 180 190 200
VLTSLNGIDE AARLADTIAA HMPLKLVDKQ QVLEIIDVTE RLEFLMGQME
210 220 230 240 250
SEIDLLQVEK RIRGRVKKQM EKSQREYYLN EQMKAIQKEL GEMEDAPDEF
260 270 280 290 300
ETLQKKIDES KMPQEAREKT EQELQKLKMM SPMSAEATVV RSYIDWMVSV
310 320 330 340 350
PWTKRSKVKK NLAKAEEILN EDHYGLERVK ERILEYLAVQ NRINKLKGPI
360 370 380 390 400
LCLVGPPGVG KTSLGRSIAS ATGRKYVRMA LGGVRDEAEI RGHRRTYIGS
410 420 430 440 450
LPGKLIQKMS KVGVKNPLFL LDEIDKMSSD MRGDPASALL EVLDPEQNNS
460 470 480 490 500
FNDHYLEVDY DLSDVMFVAT SNSMNIPGPL LDRMEVIRLS GYTEDEKLNI
510 520 530 540 550
AKRHLVEKQV QRNGLKPNEI VIEDSAIIGI IRYYTREAGV RGLEREISKI
560 570 580 590 600
CRKAVKNILL DKDIKSVTVT MDNLKEYLGV QRFDYGKADE SNRIGQVTGL
610 620 630 640 650
AWTEVGGDLL TIETQSMPGK GKLTQTGSLG DVMQESIQAA MTVVRSRADK
660 670 680 690 700
LGINSDFYEK KDIHVHVPEG ATPKDGPSAG TAMCTALVSA LTGNPVKAEV
710 720 730 740 750
AMTGEITLRG EVLPIGGLKE KLLAAHRGGI KTVLIPKDNE RDLEEIPENV
760 770 780
IADLQVIPVQ WIDEVLKVAL ERDPTGVEFE AKK
Length:783
Mass (Da):87,812
Last modified:April 4, 2003 - v2
Checksum:i723A8A81BC501778
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti136 – 1361Q → P in AAC44747 (PubMed:8981986).Curated
Sequence conflicti163 – 1631R → P in AAC44747 (PubMed:8981986).Curated
Sequence conflicti760 – 7601Q → R in AAC44747 (PubMed:8981986).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66708 Genomic DNA. Translation: AAC44747.1.
BA000031 Genomic DNA. Translation: BAC59182.1.
RefSeqiNP_797298.1. NC_004603.1.
WP_005493728.1. NC_004603.1.

Genome annotation databases

EnsemblBacteriaiBAC59182; BAC59182; BAC59182.
GeneIDi1188417.
KEGGivpa:VP0919.
PATRICi20140054. VBIVibPar50997_0871.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66708 Genomic DNA. Translation: AAC44747.1.
BA000031 Genomic DNA. Translation: BAC59182.1.
RefSeqiNP_797298.1. NC_004603.1.
WP_005493728.1. NC_004603.1.

3D structure databases

ProteinModelPortaliP74956.
SMRiP74956. Positions 8-116, 490-583, 593-783.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi223926.VP0919.

Protein family/group databases

MEROPSiS16.001.

Proteomic databases

PRIDEiP74956.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC59182; BAC59182; BAC59182.
GeneIDi1188417.
KEGGivpa:VP0919.
PATRICi20140054. VBIVibPar50997_0871.

Phylogenomic databases

eggNOGiENOG4105C6P. Bacteria.
COG0466. LUCA.
HOGENOMiHOG000261410.
KOiK01338.
OMAiGIKKAGM.
OrthoDBiEOG6XHC23.

Enzyme and pathway databases

BioCyciVPAR223926:GHK4-961-MONOMER.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01973. lon_bact.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR027543. Lon_bac.
IPR004815. Lon_bac/euk-typ.
IPR027065. Lon_Prtase.
IPR003111. LON_substr-bd_dom.
IPR027417. P-loop_NTPase.
IPR008269. Pept_S16_C.
IPR015947. PUA-like_domain.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERiPTHR10046. PTHR10046. 1 hit.
PfamiPF00004. AAA. 1 hit.
PF05362. Lon_C. 1 hit.
PF02190. LON_substr_bdg. 1 hit.
[Graphical view]
PIRSFiPIRSF001174. Lon_proteas. 1 hit.
SMARTiSM00382. AAA. 1 hit.
SM00464. LON. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00763. lon. 1 hit.
PROSITEiPS51787. LON_N. 1 hit.
PS51786. LON_PROTEOLYTIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The lonS gene regulates swarmer cell differentiation of Vibrio parahaemolyticus."
    Stewart B.J., Enos-Berlage J.L., McCarter L.L.
    J. Bacteriol. 179:107-114(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BB22.
  2. "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae."
    Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K., Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S., Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.
    Lancet 361:743-749(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RIMD 2210633.

Entry informationi

Entry nameiLON_VIBPA
AccessioniPrimary (citable) accession number: P74956
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: April 4, 2003
Last modified: February 17, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.